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6alpha-Hydroxymaackiain

CAS# 61218-44-8

6alpha-Hydroxymaackiain

Catalog No. BCN3947----Order now to get a substantial discount!

Product Name & Size Price Stock
6alpha-Hydroxymaackiain: 5mg $776 In Stock
6alpha-Hydroxymaackiain: 10mg Please Inquire In Stock
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Quality Control of 6alpha-Hydroxymaackiain

Number of papers citing our products

Chemical structure

6alpha-Hydroxymaackiain

3D structure

Chemical Properties of 6alpha-Hydroxymaackiain

Cas No. 61218-44-8 SDF Download SDF
PubChem ID 182285 Appearance Powder
Formula C16H12O6 M.Wt 300.3
Type of Compound Phenols Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
SMILES C1C2(C(C3=C(O1)C=C(C=C3)O)OC4=CC5=C(C=C42)OCO5)O
Standard InChIKey GLMPLZUBQDAZEN-JKSUJKDBSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 6alpha-Hydroxymaackiain

The herbs of Derris robusta

Biological Activity of 6alpha-Hydroxymaackiain

Description6alpha-Hydroxymaackiain is a natural product from Derris robusta.

Protocol of 6alpha-Hydroxymaackiain

Structure Identification
Canadian Journal of Botany, 1981, 59(4): 547-548.

Demethylation of the phytoalexin pisatin by Stemphylium botryosum[Reference: WebLink]

The initial product formed from pisatin by Stemphylium botryosum was identified as 3,6a-dihydroxy-8,9-methylenedioxypterocarpan(6alpha-Hydroxymaackiain) by spectrophotometric, thin-layer chromatographic and gas–liquid chromatographic comparisons with an authentic standard.

6alpha-Hydroxymaackiain Dilution Calculator

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6alpha-Hydroxymaackiain Molarity Calculator

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Preparing Stock Solutions of 6alpha-Hydroxymaackiain

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.33 mL 16.65 mL 33.3 mL 66.6001 mL 83.2501 mL
5 mM 0.666 mL 3.33 mL 6.66 mL 13.32 mL 16.65 mL
10 mM 0.333 mL 1.665 mL 3.33 mL 6.66 mL 8.325 mL
50 mM 0.0666 mL 0.333 mL 0.666 mL 1.332 mL 1.665 mL
100 mM 0.0333 mL 0.1665 mL 0.333 mL 0.666 mL 0.8325 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 6alpha-Hydroxymaackiain

Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.).[Pubmed:8951042]

Arch Biochem Biophys. 1996 Dec 1;336(1):121-9.

Isoflavone O-methyltransferase (IOMT) is a key enzyme in the biosynthesis of the phytoalexin medicarpin in alfalfa. In vivo, the B-ring 4'-hydroxyl group of the isoflavone daidzein is methylated. Surprisingly, the O-methyltransferase activity measured in vitro preferentially methylates the A-ring 7-hydroxyl group, a reaction that probably does not occur in vivo. To resolve this anomaly, we are attempting to clone the alfalfa IOMT. A substrate-based affinity chromatographic system was developed to purify the enzyme (molecular weight 41 kDa) to near homogeneity. Four internal peptide sequences were obtained from the purified protein, one of which has high (72%) sequence identity to a region of a catechol O-methyltransferase from barley. All four internal peptides, respectively, have about 55% amino acid sequence identity to four regions of 6alpha-Hydroxymaackiain 3-O-methyltransferase from Pisum sativum, but have no sequence identity to alfalfa caffeic acid 3-O-methyltransferase or chalcone 2'-O-methyltransferase. The purified IOMT has substrate specificity toward isoflavones with a free 7-hydroxyl group, but can also methylate the 5-hydroxyl group of genistein.

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