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Calcifediol monohydrate

Prehormone;vitamin D3 metabolite CAS# 63283-36-3

Calcifediol monohydrate

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Quality Control of Calcifediol monohydrate

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Chemical structure

Calcifediol monohydrate

3D structure

Chemical Properties of Calcifediol monohydrate

Cas No. 63283-36-3 SDF Download SDF
PubChem ID 6441383 Appearance Powder
Formula C27H46O3 M.Wt 418.65
Type of Compound Miscellaneous Storage Desiccate at -20°C
Synonyms 25-hydroxy Vitamin D3 monohydrate
Solubility Soluble in Chloroform
Chemical Name (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol;hydrate
SMILES CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C.O
Standard InChIKey WRLFSJXJGJBFJQ-WPUCQFJDSA-N
Standard InChI InChI=1S/C27H44O2.H2O/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29;/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3;1H2/b21-11+,22-12-;/t20-,23+,24-,25+,27-;/m1./s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Calcifediol monohydrate

Description25-Hydroxycholecalciferol supplementation at higher doses improved growth performance and decreased inflammatory gene IL-1β mRNA amounts in the liver post-LPS injection.
TargetsIL Receptor

Protocol of Calcifediol monohydrate

Animal Research

25-Hydroxycholecalciferol supplementation improves growth performance and decreases inflammation during an experimental lipopolysaccharide injection.[Pubmed: 24931970 ]

Poult Sci. 2014 Aug;93(8):1951-6.

Three experiments were conducted to study the effects of 25-hydroxycholecalciferol (25-Hydroxycholecalciferol monohydrate )supplementation on BW gain, IL-1β, and 1α-hydroxylase mRNA expression in different organs of broiler chickens following a lipopolysaccharide (LPS) injection.
METHODS AND RESULTS:
In experiment I, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 21 and 35 d of age, birds were injected with LPS. Post-LPS injection, birds supplemented with 25-hydroxycholecalciferol gained approximately 2.5% (P = 0.03) and 3.8% (P < 0.01), respectively, more BW than the birds supplemented with cholecalciferol over the 24-h period. In experiment II, birds were fed basal diets supplemented with 25-hydroxycholecalciferol at 6.25, 25, and 50 µg/kg of feed or cholecalciferol at 250 IU/kg of feed. At 35 d of age, birds were injected with LPS. Birds fed 25-hydroxycholecalciferol at 25 and 50 µg/kg and injected with LPS had approximately 7-fold and 3-fold less (P = 0.010) IL-1β mRNA in the liver compared with those birds fed 6.25 µg/kg of 25-hydroxycholecalciferol and the cholecalciferol (250 IU/kg) group. In experiment III, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 28 d of age, birds were fed 25-hydroxycholecalciferol and injected with LPS had 1.1-fold less (P < 0.01) IL-1β mRNA in the liver than the cholecalciferol-fed group. After an LPS injection, birds supplemented with 25-hydroxycholecalciferol had increased 1α-hydroxylase mRNA amounts in the liver (P = 0.07).
CONCLUSIONS:
In conclusion, 25-hydroxycholecalciferol supplementation at higher doses improved growth performance and decreased inflammatory gene IL-1β mRNA amounts in the liver post-LPS injection.

Structure Identification
Biochemical and Biophysical Research Communications, 1980, 95(1):177-181.

Isolation and identification of 25-hydroxy-24-oxocholecalciferol: A metabolite of 25-hydroxycholecalciferol[Reference: WebLink]

A metabolite of 25-hydroxycholecalciferol(25-Hydroxycholecalciferol monohydrate ) has been isolated in pure form from chicken kidney homogenates.
METHODS AND RESULTS:
It has been identified as 25-hydroxy-24-oxocholecalciferol by means of ultraviolet absorption spectrophotometry, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and specific chemical reactions.

J Agric Food Chem. 1979 May-Jun;27(3):650-2.

25-Hydroxycholecalciferol in cow milk as determined by high-performance liquid chromatography[Reference: WebLink]


METHODS AND RESULTS:
A high-performance liquid Chromatographic (LC) procedure was developed for the determination of 25-hydroxycholecalciferol (25-Hydroxycholecalciferol monohydrate,25-OH-D3) in cow milk. The procedure involved extraction with an ethanol-ether mixture, a set of solvent partitions, adsorption chromatography on silica gel, a partition chromatography on diatomaceous earth support, and final determination by reversed-phase LC on a C18 bonded microparticulate silica column using a 254-nm fixed wavelength detector. The method was quantitative at the 10-ppb level and capable of detection at the 2-3-ppb level.
CONCLUSIONS:
The method was applicable to milk and colostrum. The endogenous level of 25-OH-D3 in the milk was below the detection limit. It was very significant that, even in cows whose serum concentrations were elevated fivefold after treatment with 25-OH-D3, the concentration in the milk was below the detection level.

Calcifediol monohydrate Dilution Calculator

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Calcifediol monohydrate Molarity Calculator

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Preparing Stock Solutions of Calcifediol monohydrate

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.3886 mL 11.9432 mL 23.8863 mL 47.7726 mL 59.7158 mL
5 mM 0.4777 mL 2.3886 mL 4.7773 mL 9.5545 mL 11.9432 mL
10 mM 0.2389 mL 1.1943 mL 2.3886 mL 4.7773 mL 5.9716 mL
50 mM 0.0478 mL 0.2389 mL 0.4777 mL 0.9555 mL 1.1943 mL
100 mM 0.0239 mL 0.1194 mL 0.2389 mL 0.4777 mL 0.5972 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on Calcifediol monohydrate

Calcifediol is a prehormone that is produced in the liver by hydroxylation of vitamin D3 (cholecalciferol) and is the best indicator of total vitamin D exposure [1].
The main circulating form of vitamin D, higher serum levels of calcifediol is associated with lower incidence rates of colon, breast, ovarian, renal, pancreatic, aggressive prostate and other cancers [2].
In bovine parathyroid cells, Calcifediol can reduce parathyroid hormone (PTH) secretion and mRNA synthesis [3]. In T-47D breast cancer cells, calcifediol mediated activation of VDR (the vitamin D receptor), which was associated with endocytosis of DBP (vitamin D binding protein). So T-47D breast cancer cells can rapidly internalize DBP [4].
In the pooled study included 616 colorectal adenoma cases and 770 controls, the association between circulating 25(OH)D3 and colorectal adenoma risk was estimated through multivariable logistic regression. The higher circulating 25(OH)D3 concentrations were significantly associated with lower colorectal adenoma risk [1].
References:
[1]. Fedirko V, Bostick RM, Goodman M, et al. Blood 25-hydroxyvitamin D3 concentrations and incident sporadic colorectal adenoma risk: a pooled case-control study. Am J Epidemiol, 2010, 172(5): 489-500.
[2]. Garland CF, Gorham ED, Mohr SB, et al. Vitamin D for cancer prevention: global perspective. Ann Epidemiol, 2009, 19(7): 468-483.
[3]. Ritter CS, Armbrecht HJ, Slatopolsky E, et al. 25-Hydroxyvitamin D(3) suppresses PTH synthesis and secretion by bovine parathyroid cells. Kidney Int, 2006, 70(4): 654-659.
[4]. Rowling MJ, Kemmis CM, Taffany DA, et al. Megalin-mediated endocytosis of vitamin D binding protein correlates with 25-hydroxycholecalciferol actions in human mammary cells. J Nutr, 2006, 36(11): 2754-2759.

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Description

Calcifediol monohydrate(25-hydroxy Vitamin D3) is a prehormone that is produced in the liver by hydroxylation of vitamin D3 (cholecalciferol) by the enzyme cholecalciferol 25-hydroxylase IC50 value: Target: This metabolite is being measured by physicians worldwide to determine a patient's vitamin d status.

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