Ac-IEPD-AFC

Recognition motif for serine protease granzyme B CAS# 1135417-31-0

Ac-IEPD-AFC

Catalog No. BCC2358----Order now to get a substantial discount!

Product Name & Size Price Stock
Ac-IEPD-AFC: 5mg $196 In Stock
Ac-IEPD-AFC: 10mg Please Inquire In Stock
Ac-IEPD-AFC: 20mg Please Inquire Please Inquire
Ac-IEPD-AFC: 50mg Please Inquire Please Inquire
Ac-IEPD-AFC: 100mg Please Inquire Please Inquire
Ac-IEPD-AFC: 200mg Please Inquire Please Inquire
Ac-IEPD-AFC: 500mg Please Inquire Please Inquire
Ac-IEPD-AFC: 1000mg Please Inquire Please Inquire
Related Products

Quality Control of Ac-IEPD-AFC

Number of papers citing our products

Chemical structure

Ac-IEPD-AFC

3D structure

Chemical Properties of Ac-IEPD-AFC

Cas No. 1135417-31-0 SDF Download SDF
PubChem ID 25108575 Appearance Powder
Formula C32H38F3N5O11 M.Wt 725.67
Type of Compound N/A Storage Desiccate at -20°C
Solubility Soluble to 14.51 mg/ml in DMSO
Sequence IEPD

(Modifications: Ile-1 = N-Ac-Ile, Asp-4 = Asp-(7-amino-4-trifluoromethylcoumarin))

Chemical Name (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[(2S)-2-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]carbamoyl]pyrrolidin-1-yl]-5-oxopentanoic acid
SMILES CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C
Standard InChIKey HWXKKVLJPWMVLL-FGGUPADCSA-N
Standard InChI InChI=1S/C32H38F3N5O11/c1-4-15(2)27(36-16(3)41)30(49)38-20(9-10-24(42)43)31(50)40-11-5-6-22(40)29(48)39-21(14-25(44)45)28(47)37-17-7-8-18-19(32(33,34)35)13-26(46)51-23(18)12-17/h7-8,12-13,15,20-22,27H,4-6,9-11,14H2,1-3H3,(H,36,41)(H,37,47)(H,38,49)(H,39,48)(H,42,43)(H,44,45)/t15-,20-,21-,22-,27-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Ac-IEPD-AFC

DescriptionPeptide sequence shown to be preferred recognition motif for the serine protease granzyme B. Fluorogenic substrate.

Ac-IEPD-AFC Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Ac-IEPD-AFC Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on Ac-IEPD-AFC

Ac-LEHD-AFC is a fluorogenic substrate for caspase-4, caspase-5, and caspase-9.

Featured Products
New Products
 

References on Ac-IEPD-AFC

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.[Pubmed:9218414]

J Biol Chem. 1997 Jul 18;272(29):17907-11.

There is compelling evidence that members of the caspase (interleukin-1beta converting enzyme/CED-3) family of cysteine proteases and the cytotoxic lymphocyte-derived serine protease granzyme B play essential roles in mammalian apoptosis. Here we use a novel method employing a positional scanning substrate combinatorial library to rigorously define their individual specificities. The results divide these proteases into three distinct groups and suggest that several have redundant functions. The specificity of caspases 2, 3, and 7 and Caenorhabditis elegans CED-3 (DEXD) suggests that all of these enzymes function to incapacitate essential homeostatic pathways during the effector phase of apoptosis. In contrast, the optimal sequence for caspases 6, 8, and 9 and granzyme B ((I/L/V)EXD) resembles activation sites in effector caspase proenzymes, consistent with a role for these enzymes as upstream components in a proteolytic cascade that amplifies the death signal.

Description

Ac-IEPD-AFC (IEPD) is a substrate of Granzyme B.

Keywords:

Ac-IEPD-AFC,1135417-31-0,Natural Products,Caspase, buy Ac-IEPD-AFC , Ac-IEPD-AFC supplier , purchase Ac-IEPD-AFC , Ac-IEPD-AFC cost , Ac-IEPD-AFC manufacturer , order Ac-IEPD-AFC , high purity Ac-IEPD-AFC

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: