Home >> Research Area >>PI3K/Akt/mTOR Signaling>>Akt>> GSK2141795 hydrochloride

GSK2141795 hydrochloride

CAS# 1047635-80-2

GSK2141795 hydrochloride

Catalog No. BCC5295----Order now to get a substantial discount!

Product Name & Size Price Stock
GSK2141795 hydrochloride: 5mg $897 In Stock
GSK2141795 hydrochloride: 10mg Please Inquire In Stock
GSK2141795 hydrochloride: 20mg Please Inquire Please Inquire
GSK2141795 hydrochloride: 50mg Please Inquire Please Inquire
GSK2141795 hydrochloride: 100mg Please Inquire Please Inquire
GSK2141795 hydrochloride: 200mg Please Inquire Please Inquire
GSK2141795 hydrochloride: 500mg Please Inquire Please Inquire
GSK2141795 hydrochloride: 1000mg Please Inquire Please Inquire
Related Products
  • PP242

    Catalog No.:BCC3682
    CAS No.:1092351-67-1
  • A66

    Catalog No.:BCC3715
    CAS No.:1166227-08-2
  • CAL-101 (Idelalisib, GS-1101)

    Catalog No.:BCC1270
    CAS No.:870281-82-6
  • PIK-294

    Catalog No.:BCC4995
    CAS No.:900185-02-6
  • OSI-027

    Catalog No.:BCC4603
    CAS No.:936890-98-1
  • KU-0063794

    Catalog No.:BCC2484
    CAS No.:938440-64-3

Quality Control of GSK2141795 hydrochloride

Number of papers citing our products

Chemical structure

GSK2141795 hydrochloride

3D structure

Chemical Properties of GSK2141795 hydrochloride

Cas No. 1047635-80-2 SDF Download SDF
PubChem ID 73330428 Appearance Powder
Formula C18H17Cl3F2N4O2 M.Wt 465.71
Type of Compound N/A Storage Desiccate at -20°C
Synonyms Uprosertib hydrochloride
Solubility Soluble in DMSO
Chemical Name N-[(2S)-1-amino-3-(3,4-difluorophenyl)propan-2-yl]-5-chloro-4-(4-chloro-2-methylpyrazol-3-yl)furan-2-carboxamide;hydrochloride
SMILES CN1C(=C(C=N1)Cl)C2=C(OC(=C2)C(=O)NC(CC3=CC(=C(C=C3)F)F)CN)Cl.Cl
Standard InChIKey LAPFKCIDRPWAFU-PPHPATTJSA-N
Standard InChI InChI=1S/C18H16Cl2F2N4O2.ClH/c1-26-16(12(19)8-24-26)11-6-15(28-17(11)20)18(27)25-10(7-23)4-9-2-3-13(21)14(22)5-9;/h2-3,5-6,8,10H,4,7,23H2,1H3,(H,25,27);1H/t10-;/m0./s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Protocol

Kinase Assay [1]
For selectivity profiling experiments, the lysates (5 mg of total protein each) are preincubated with 0 (DMSO control), 2.5 nM, 25 nM, 250 nM, 2.5 μM or 25 μM free compound (GSK690693 or GSK2141795) on an end-over-end shaker for 45 min at 4°C. Subsequently, lysates are incubated with beads (coupled Akt probe or kinobeads) for 1 h at 4°C, for both qualitative and quantitative experiments. The beads are washed with 1×CP buffer and collected by centrifugation. Bound proteins are eluted with 2×NuPAGE LDS sample buffer, and eluates are reduced and alkylated by 50 mM dithiothreitol and 55 mM iodoacetamide.

References:
[1]. Pachl F, et al. Characterization of a chemical affinity probe targeting Akt kinases. J Proteome Res. 2013 Aug 2;12(8):3792-800.

GSK2141795 hydrochloride Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

GSK2141795 hydrochloride Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of GSK2141795 hydrochloride

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.1473 mL 10.7363 mL 21.4726 mL 42.9452 mL 53.6815 mL
5 mM 0.4295 mL 2.1473 mL 4.2945 mL 8.589 mL 10.7363 mL
10 mM 0.2147 mL 1.0736 mL 2.1473 mL 4.2945 mL 5.3681 mL
50 mM 0.0429 mL 0.2147 mL 0.4295 mL 0.8589 mL 1.0736 mL
100 mM 0.0215 mL 0.1074 mL 0.2147 mL 0.4295 mL 0.5368 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on GSK2141795 hydrochloride

GSK2141795 hydrochloride is a potent and selective pan-Akt inhibitor with IC50 values of 180/328/38 nM for Akt1/Akt2/Akt3, respectively.

In Vitro:GSK2141795 inhibits Akt1/2/3 with the Kd values of 16/49/5 nM, respectively. GSK2141795 potently inhibits only the PKC family members PRKACA and PRKACB as well as the cGMP-dependent protein kinase PRKG1 aqpart from the Akts. Protein targets that bind GSK2141795 in the lysate show a dose-dependent reduction in binding to the kinobeads, while proteins unaffected by the drug show no reduction in binding[1].

References:
[1]. Pachl F, et al. Characterization of a chemical affinity probe targeting Akt kinases. J Proteome Res. 2013 Aug 2;12(8):3792-800.

Featured Products
New Products
 

Description

GSK2141795 Hcl is a potent and selective pan-Akt inhibitor with IC50 values of 180 nM for Akt1, 328 nM for Akt2, and 38 nM for Akt3 respectively (Clinical phase 1).

Keywords:

GSK2141795 hydrochloride,1047635-80-2,Uprosertib hydrochloride,Natural Products,Akt, buy GSK2141795 hydrochloride , GSK2141795 hydrochloride supplier , purchase GSK2141795 hydrochloride , GSK2141795 hydrochloride cost , GSK2141795 hydrochloride manufacturer , order GSK2141795 hydrochloride , high purity GSK2141795 hydrochloride

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: