Proline

CAS# 147-85-3

Proline

Catalog No. BCN1656----Order now to get a substantial discount!

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Quality Control of Proline

Number of papers citing our products

Chemical structure

Proline

3D structure

Chemical Properties of Proline

Cas No. 147-85-3 SDF Download SDF
PubChem ID 145742 Appearance White powder
Formula C5H9NO2 M.Wt 115.1
Type of Compound Alkaloids Storage Desiccate at -20°C
Synonyms (S)-2-Carboxypyrrolidine; (S)-2-Pyrrolidinecarboxylic acid
Solubility Soluble in water
Chemical Name (2S)-pyrrolidine-2-carboxylic acid
SMILES C1CC(NC1)C(=O)O
Standard InChIKey ONIBWKKTOPOVIA-BYPYZUCNSA-N
Standard InChI InChI=1S/C5H9NO2/c7-5(8)4-2-1-3-6-4/h4,6H,1-3H2,(H,7,8)/t4-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Proline

The herbs of Gynura japonica

Biological Activity of Proline

DescriptionL-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.Proline accumulates in many plant species in response to environmental stress, it can act as a signaling molecule to modulate mitochondrial functions, influence cell proliferation or cell death and trigger specific gene expression, which can be essential for plant recovery from stress, the engineering of proline metabolism could lead to new opportunities to improve plant tolerance of environmental stresses.
Targetsp38MAPK | HIF
In vitro

HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing.[Pubmed: 11292862 ]

Science. 2001 Apr 20;292(5516):464-8.

HIF (hypoxia-inducible factor) is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability. In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel-Lindau tumor suppressor protein (pVHL). We found that human pVHL binds to a short HIF-derived peptide when a conserved Proline residue at the core of this peptide is hydroxylated. Because Proline hydroxylation requires molecular oxygen and Fe(2+), this protein modification may play a key role in mammalian oxygen sensing.

In vivo

Proline: a multifunctional amino acid.[Pubmed: 20036181 ]

Trends Plant Sci. 2010 Feb;15(2):89-97.

Proline accumulates in many plant species in response to environmental stress. Although much is now known about Proline metabolism, some aspects of its biological functions are still unclear. Here, we discuss the compartmentalization of Proline biosynthesis, accumulation and degradation in the cytosol, chloroplast and mitochondria. We also describe the role of Proline in cellular homeostasis, including redox balance and energy status. Proline can act as a signaling molecule to modulate mitochondrial functions, influence cell proliferation or cell death and trigger specific gene expression, which can be essential for plant recovery from stress. Although the regulation and function of Proline accumulation are not yet completely understood, the engineering of Proline metabolism could lead to new opportunities to improve plant tolerance of environmental stresses.

Protocol of Proline

Structure Identification
Cell. 2006 Sep 8;126(5):905-16.

Proline isomerization of histone H3 regulates lysine methylation and gene expression.[Pubmed: 16959570 ]

The cis-trans isomerization of Proline serves as a regulatory switch in signaling pathways. We identify the Proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 Proline P30 and P38 in vitro. We show that P38 is necessary for methylation of K36 and that isomerization by Fpr4 inhibits the ability of Set2 to methylate H3 K36 in vitro. These results suggest that the conformational state of P38, controlled by Fpr4, is important for methylation of H3K36 by Set2. Consistent with such an antagonistic role, abrogation of Fpr4 catalytic activity in vivo results in increased levels of H3K36 methylation and delayed transcriptional induction kinetics of specific genes in yeast. These results identify Proline isomerization as a novel noncovalent histone modification that regulates transcription and provides evidence for crosstalk between histone lysine methylation and Proline isomerization.

Proline Dilution Calculator

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Proline Molarity Calculator

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Preparing Stock Solutions of Proline

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.6881 mL 43.4405 mL 86.881 mL 173.7619 mL 217.2024 mL
5 mM 1.7376 mL 8.6881 mL 17.3762 mL 34.7524 mL 43.4405 mL
10 mM 0.8688 mL 4.344 mL 8.6881 mL 17.3762 mL 21.7202 mL
50 mM 0.1738 mL 0.8688 mL 1.7376 mL 3.4752 mL 4.344 mL
100 mM 0.0869 mL 0.4344 mL 0.8688 mL 1.7376 mL 2.172 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

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Yale University

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The Institute of Cancer Research

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Universite de Paris
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The University of Tokyo
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Korea University
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Background on Proline

H-Pro-OH

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References on Proline

Proline: a multifunctional amino acid.[Pubmed:20036181]

Trends Plant Sci. 2010 Feb;15(2):89-97.

Proline accumulates in many plant species in response to environmental stress. Although much is now known about Proline metabolism, some aspects of its biological functions are still unclear. Here, we discuss the compartmentalization of Proline biosynthesis, accumulation and degradation in the cytosol, chloroplast and mitochondria. We also describe the role of Proline in cellular homeostasis, including redox balance and energy status. Proline can act as a signaling molecule to modulate mitochondrial functions, influence cell proliferation or cell death and trigger specific gene expression, which can be essential for plant recovery from stress. Although the regulation and function of Proline accumulation are not yet completely understood, the engineering of Proline metabolism could lead to new opportunities to improve plant tolerance of environmental stresses.

HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing.[Pubmed:11292862]

Science. 2001 Apr 20;292(5516):464-8.

HIF (hypoxia-inducible factor) is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability. In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel-Lindau tumor suppressor protein (pVHL). We found that human pVHL binds to a short HIF-derived peptide when a conserved Proline residue at the core of this peptide is hydroxylated. Because Proline hydroxylation requires molecular oxygen and Fe(2+), this protein modification may play a key role in mammalian oxygen sensing.

Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response.[Pubmed:1623521]

Cell. 1992 Jul 10;70(1):21-30.

Treatment of bean or soybean cells with fungal elicitor or glutathione causes a rapid insolubilization of preexisting (hydroxy)Proline-rich structural proteins in the cell wall. This insolubilization, which involves H2O2-mediated oxidative cross-linking, is initiated within 2 min and is complete within 10 min under optimal conditions, and hence, precedes the expression of transcription-dependent defenses. Cross-linking is also under developmental control during hypocotyl growth and in tissues subject to mechanical stress such as the stem-petiole junction. Stimulus-dependent oxidative cross-linking of wall structural proteins is a novel site of cellular regulation with potentially important functions in cell maturation and toughening of cell walls in the initial stages of plant defense.

Proline isomerization of histone H3 regulates lysine methylation and gene expression.[Pubmed:16959570]

Cell. 2006 Sep 8;126(5):905-16.

The cis-trans isomerization of Proline serves as a regulatory switch in signaling pathways. We identify the Proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 Proline P30 and P38 in vitro. We show that P38 is necessary for methylation of K36 and that isomerization by Fpr4 inhibits the ability of Set2 to methylate H3 K36 in vitro. These results suggest that the conformational state of P38, controlled by Fpr4, is important for methylation of H3K36 by Set2. Consistent with such an antagonistic role, abrogation of Fpr4 catalytic activity in vivo results in increased levels of H3K36 methylation and delayed transcriptional induction kinetics of specific genes in yeast. These results identify Proline isomerization as a novel noncovalent histone modification that regulates transcription and provides evidence for crosstalk between histone lysine methylation and Proline isomerization.

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