Estriol

CAS# 50-27-1

Estriol

Catalog No. BCN2235----Order now to get a substantial discount!

Product Name & Size Price Stock
Estriol: 5mg $23 In Stock
Estriol: 10mg Please Inquire In Stock
Estriol: 20mg Please Inquire Please Inquire
Estriol: 50mg Please Inquire Please Inquire
Estriol: 100mg Please Inquire Please Inquire
Estriol: 200mg Please Inquire Please Inquire
Estriol: 500mg Please Inquire Please Inquire
Estriol: 1000mg Please Inquire Please Inquire
Related Products

Quality Control of Estriol

Number of papers citing our products

Chemical structure

Estriol

3D structure

Chemical Properties of Estriol

Cas No. 50-27-1 SDF Download SDF
PubChem ID 5756 Appearance White cryst.
Formula C18H24O3 M.Wt 288.38
Type of Compound Steroids Storage Desiccate at -20°C
Solubility Soluble in DMSO > 10 mM
Chemical Name (8R,9S,13S,14S,16R,17R)-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,16,17-triol
SMILES CC12CCC3C(C1CC(C2O)O)CCC4=C3C=CC(=C4)O
Standard InChIKey PROQIPRRNZUXQM-ZXXIGWHRSA-N
Standard InChI InChI=1S/C18H24O3/c1-18-7-6-13-12-5-3-11(19)8-10(12)2-4-14(13)15(18)9-16(20)17(18)21/h3,5,8,13-17,19-21H,2,4,6-7,9H2,1H3/t13-,14-,15+,16-,17+,18+/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Estriol

DescriptionEstriol (E3), an endogenous estrogen predominantly produced during human pregnancy, is an antagonist of the G-protein coupled estrogen receptor in estrogen receptor-negative breast cancer cells. E3 can blunt the postprandial glycemic surge in rats through modulating the level of intestinal glucose transporters. E3 exerts a mitogenic effect on T-47D and MCF-7 cells at concentrations of 10(-9)M (288 pg/ml) and higher.
TargetsEstrogen receptor | Progestogen receptor | G-protein coupled estrogen receptor
In vitro

Single measurement of salivary estriol as a predictor of preterm birth.[Pubmed: 26031009]

Pak J Biol Sci. 2014 May;17(5):730-4.

One of the major problems in obstetrics and pediatrics is preterm birth. A new method of prediction of preterm birth is by salivary Estriol. This study aimed to determine the predictive value of single measurement of salivary Estriol and its relationship with preterm birth.
METHODS AND RESULTS:
In this study, the salivary specimens of 466 pregnant women of 25-34 weeks gestational age were collected and kept in a freezer until delivery. Consequently, the salivary specimens were thawed and Estriol levels were measured. The cut-off point for Estriol was determined by a receiver operating characteristics curve. Salivary Estriol levels equal to or higher than the cut-off point (2.6 ng m(-1)) were considered as the Estriol (+) group and those lower than 2.6 ng mL(-1) were considered as the Estriol (-) group. Our findings showed that 36 (18.3%) subjects in the Estriol (+) group and 22 (8.2%) subjects in the Estriol (-) group had preterm deliveries. There was a significant relationship between salivary Estriol levels and preterm birth (χ2 = 10.636, p = 0.001). Sensitivity, specificity and predictive values (positive and negative) of Estriol were 62, 60, 18.3 and 82%, respectively. Single measurement of salivary Estriol at 25-34 weeks of gestation, with its high negative predictive values, could be beneficial to identify women who will not develop preterm labor.
CONCLUSIONS:
This outcome suggests that unnecessary interventions should be avoided to prevent preterm births.

Effect of Escherichia coli secreted metabolites on functional activity of human neutrophils against the background of estriol effect.[Pubmed: 25536774]

Zh Mikrobiol Epidemiol Immunobiol. 2014 Sep-Oct;(5):65-70.

Study the effect of Escherichia coli acellular metabolites of various phases of development on phagocytic activity of neutrophils against the background of pregnancy hormone effect--Estriol (E3).
METHODS AND RESULTS:
E. coli K12 (wt) metabolites were selected at the end of adaptation and logarithmic growth phases by filtration after cultivation at 37 degrees C in LB broth. Neutrophils of heparinized venous blood of healthy non-pregnant women (follicular phase, n = 8) were incubated for 1 hour with E3 at 2 ng/ml and 20 ng/ml, then 30 minutes with E. coli metabolites, LB medium or Hanks' solution at 37 degrees C. Phagocytic activity evaluation was carried out by inhibition of bioluminescence of E. coli K12 TG1 lux+. Exometabolites of logarithmic growth phase of E. coli culture inhibited neutrophil phagocytosis after 40 - 60 minutes of incubation in contrast to metabolites of adaptation phase compared with LB medium. Neutrophil cultivation after hormone treatment in LB medium that has the ability to stimulate neutrophil phagocytosis compared with Hanks' solution did not alter their phagocytic activity. However inhibiting effect of E3 at 20 ng/ml on neutrophil phagocytosis compared with control was exhibited in Hanks' solution (at 50 - 60 minutes) and after the effect of E. coli adaptation phase metabolites (at 40 - 60 minutes).
CONCLUSIONS:
E3 at the level extrapolated from its level at III trimester of pregnancy could facilitate the reduction of antimicrobial potential of neutrophils at the early stages of growth of pathogenic microorganisms.

In vivo

Estriol blunts postprandial blood glucose rise in male rats through regulating intestinal glucose transporters.[Pubmed: 25516546]

Am J Physiol Endocrinol Metab. 2015 Mar 1;308(5):E370-9.

Despite increased total food intake in healthy, late-stage pregnant women, their peak postprandial blood sugar levels are normally much lower than the levels seen in healthy nonpregnant women.
METHODS AND RESULTS:
In this study, we sought to determine whether Estriol (E3), an endogenous estrogen predominantly produced during human pregnancy, contributes to the regulation of the postprandial blood glucose level in healthy normal rats. In vivo studies using rats showed that E3 blunted the speed and magnitude of the blood glucose rise following oral glucose administration, but it did not appear to affect the total amount of glucose absorbed. E3 also did not affect insulin secretion, but it significantly reduced the rate of intestinal glucose transport compared with vehicle-treated animals. Consistent with this finding, expression of the sodium-dependent glucose transporter 1 and 2 was significantly downregulated by E3 treatment in the brush-border membrane and basolateral membrane, respectively, of enterocytes. Most of the observed in vivo effects were noticeably stronger with E3 than with 17β-estradiol. Using differentiated human Caco-2 enterocyte monolayer culture as an in vitro model, we confirmed that E3 at physiologically relevant concentrations could directly inhibit glucose uptake via suppression of glucose transporter 2 expression, whereas 17β-estradiol did not have a similar effect.
CONCLUSIONS:
Collectively, these data showed that E3 can blunt the postprandial glycemic surge in rats through modulating the level of intestinal glucose transporters.

Protocol of Estriol

Cell Research

Effects of estriol on growth, gene expression and estrogen response element activation in human breast cancer cell lines.[Pubmed: 24529907]

Maturitas. 2014 Apr;77(4):336-43.

Local application of estradiol (E2) to treat vulvovaginal atrophy in postmenopausal breast cancer patients receiving aromatase inhibitors is known to elevate serum estradiol levels and thereby might counteract breast cancer therapy. Thus, vaginal application of Estriol (E3) has been recommended for these patients. However, it is unclear to what extent E3 stimulates breast cancer cell growth. In this study, we examined the effect of E3 on growth and gene expression of two human breast cancer cell lines.
METHODS AND RESULTS:
We used an established in vitro cell culture assay and compared the effect of E2 and E3 on growth of the estrogen receptor alpha-positive breast cancer cell lines MCF-7 and T-47D testing a wide range of hormone concentrations of 10(-12)-10(-7)M. E3 effects on gene expression were examined by means of reporter gene assays, RT-qPCR and Western blot analysis. E3 acted as a potent estrogen and exerted a mitogenic effect on T-47D and MCF-7 cells at concentrations of 10(-9)M (288 pg/ml) and higher. With regard to activation of an estrogen response element (ERE) in breast cancer cells, effects of E3 were visible at 10(-10)M. The same concentrations of E3 activated expression of the estrogen-responsive gene PR and of the proliferation genes cyclin A2, cyclin B1, Ki-67, c-myc and b-myb, providing molecular mechanisms underlying the observed growth increase.
CONCLUSIONS:
Like E2, low levels of E3 were able to trigger a robust estrogenic response in breast cancer cells. Thus, our data suggest caution regarding use of E3 by breast cancer survivors.

Estriol Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Estriol Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Estriol

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.4676 mL 17.3382 mL 34.6765 mL 69.3529 mL 86.6912 mL
5 mM 0.6935 mL 3.4676 mL 6.9353 mL 13.8706 mL 17.3382 mL
10 mM 0.3468 mL 1.7338 mL 3.4676 mL 6.9353 mL 8.6691 mL
50 mM 0.0694 mL 0.3468 mL 0.6935 mL 1.3871 mL 1.7338 mL
100 mM 0.0347 mL 0.1734 mL 0.3468 mL 0.6935 mL 0.8669 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on Estriol

Estriol is an antagonist of the G-protein coupled estrogen receptor in estrogen receptor-negative breast cancer cells.

Featured Products
New Products
 

References on Estriol

[Effect of Escherichia coli secreted metabolites on functional activity of human neutrophils against the background of estriol effect].[Pubmed:25536774]

Zh Mikrobiol Epidemiol Immunobiol. 2014 Sep-Oct;(5):65-70.

AIM: Study the effect of Escherichia coli acellular metabolites of various phases of development on phagocytic activity of neutrophils against the background of pregnancy hormone effect--Estriol (E3). MATERIALS AND METHODS: E. coli K12 (wt) metabolites were selected at the end of adaptation and logarithmic growth phases by filtration after cultivation at 37 degrees C in LB broth. Neutrophils of heparinized venous blood of healthy non-pregnant women (follicular phase, n = 8) were incubated for 1 hour with E3 at 2 ng/ml and 20 ng/ml, then 30 minutes with E. coli metabolites, LB medium or Hanks' solution at 37 degrees C. Phagocytic activity evaluation was carried out by inhibition of bioluminescence of E. coli K12 TG1 lux+. RESULTS: Exometabolites of logarithmic growth phase of E. coli culture inhibited neutrophil phagocytosis after 40 - 60 minutes of incubation in contrast to metabolites of adaptation phase compared with LB medium. Neutrophil cultivation after hormone treatment in LB medium that has the ability to stimulate neutrophil phagocytosis compared with Hanks' solution did not alter their phagocytic activity. However inhibiting effect of E3 at 20 ng/ml on neutrophil phagocytosis compared with control was exhibited in Hanks' solution (at 50 - 60 minutes) and after the effect of E. coli adaptation phase metabolites (at 40 - 60 minutes). CONCLUSION: E3 at the level extrapolated from its level at III trimester of pregnancy could facilitate the reduction of antimicrobial potential of neutrophils at the early stages of growth of pathogenic microorganisms.

Single measurement of salivary estriol as a predictor of preterm birth.[Pubmed:26031009]

Pak J Biol Sci. 2014 May;17(5):730-4.

One of the major problems in obstetrics and pediatrics is preterm birth. A new method of prediction of preterm birth is by salivary Estriol. This study aimed to determine the predictive value of single measurement of salivary Estriol and its relationship with preterm birth. In this study, the salivary specimens of 466 pregnant women of 25-34 weeks gestational age were collected and kept in a freezer until delivery. Consequently, the salivary specimens were thawed and Estriol levels were measured. The cut-off point for Estriol was determined by a receiver operating characteristics curve. Salivary Estriol levels equal to or higher than the cut-off point (2.6 ng m(-1)) were considered as the Estriol (+) group and those lower than 2.6 ng mL(-1) were considered as the Estriol (-) group. Our findings showed that 36 (18.3%) subjects in the Estriol (+) group and 22 (8.2%) subjects in the Estriol (-) group had preterm deliveries. There was a significant relationship between salivary Estriol levels and preterm birth (chi2 = 10.636, p = 0.001). Sensitivity, specificity and predictive values (positive and negative) of Estriol were 62, 60, 18.3 and 82%, respectively. Single measurement of salivary Estriol at 25-34 weeks of gestation, with its high negative predictive values, could be beneficial to identify women who will not develop preterm labor. This outcome suggests that unnecessary interventions should be avoided to prevent preterm births.

Effects of estriol on growth, gene expression and estrogen response element activation in human breast cancer cell lines.[Pubmed:24529907]

Maturitas. 2014 Apr;77(4):336-43.

OBJECTIVE: Local application of estradiol (E2) to treat vulvovaginal atrophy in postmenopausal breast cancer patients receiving aromatase inhibitors is known to elevate serum estradiol levels and thereby might counteract breast cancer therapy. Thus, vaginal application of Estriol (E3) has been recommended for these patients. However, it is unclear to what extent E3 stimulates breast cancer cell growth. In this study, we examined the effect of E3 on growth and gene expression of two human breast cancer cell lines. METHODS: We used an established in vitro cell culture assay and compared the effect of E2 and E3 on growth of the estrogen receptor alpha-positive breast cancer cell lines MCF-7 and T-47D testing a wide range of hormone concentrations of 10(-12)-10(-7)M. E3 effects on gene expression were examined by means of reporter gene assays, RT-qPCR and Western blot analysis. RESULTS: E3 acted as a potent estrogen and exerted a mitogenic effect on T-47D and MCF-7 cells at concentrations of 10(-9)M (288 pg/ml) and higher. With regard to activation of an estrogen response element (ERE) in breast cancer cells, effects of E3 were visible at 10(-10)M. The same concentrations of E3 activated expression of the estrogen-responsive gene PR and of the proliferation genes cyclin A2, cyclin B1, Ki-67, c-myc and b-myb, providing molecular mechanisms underlying the observed growth increase. CONCLUSIONS: Like E2, low levels of E3 were able to trigger a robust estrogenic response in breast cancer cells. Thus, our data suggest caution regarding use of E3 by breast cancer survivors.

Estriol blunts postprandial blood glucose rise in male rats through regulating intestinal glucose transporters.[Pubmed:25516546]

Am J Physiol Endocrinol Metab. 2015 Mar 1;308(5):E370-9.

Despite increased total food intake in healthy, late-stage pregnant women, their peak postprandial blood sugar levels are normally much lower than the levels seen in healthy nonpregnant women. In this study, we sought to determine whether Estriol (E3), an endogenous estrogen predominantly produced during human pregnancy, contributes to the regulation of the postprandial blood glucose level in healthy normal rats. In vivo studies using rats showed that E3 blunted the speed and magnitude of the blood glucose rise following oral glucose administration, but it did not appear to affect the total amount of glucose absorbed. E3 also did not affect insulin secretion, but it significantly reduced the rate of intestinal glucose transport compared with vehicle-treated animals. Consistent with this finding, expression of the sodium-dependent glucose transporter 1 and 2 was significantly downregulated by E3 treatment in the brush-border membrane and basolateral membrane, respectively, of enterocytes. Most of the observed in vivo effects were noticeably stronger with E3 than with 17beta-estradiol. Using differentiated human Caco-2 enterocyte monolayer culture as an in vitro model, we confirmed that E3 at physiologically relevant concentrations could directly inhibit glucose uptake via suppression of glucose transporter 2 expression, whereas 17beta-estradiol did not have a similar effect. Collectively, these data showed that E3 can blunt the postprandial glycemic surge in rats through modulating the level of intestinal glucose transporters.

Description

Estriol is an antagonist of the G-protein coupled estrogen receptor in estrogen receptor-negative breast cancer cells.

Keywords:

Estriol,50-27-1,Natural Products,Estrogen/progestogen Receptor, buy Estriol , Estriol supplier , purchase Estriol , Estriol cost , Estriol manufacturer , order Estriol , high purity Estriol

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: