Cdc42 N17, Recombinant ProteinCatalog# BCP38294 |
| Product Name | Cdc42 N17, Recombinant Protein | ||
| Full Product Name | Cdc42 N17 (Dominant Negative) Recombinant Adenovirus | ||
| Product Synonym Names | Cdc42 N17 Recombinant Adenovirus (Dominant Negative) | ||
| Research Use Only | For Research Use Only. Not for use in diagnostic procedures. | ||
| Concentration | 50 ul, 1 x 10^11 VP/mL in TBS containing 10% Glycerol (lot specific) | ||
| Methods | The appropriate amount of viruses used for infecting cells is critical for the outcome of your experiments. If not enough virus is used, it will not give 100% of infection. If too much virus is used, it will cause cytotoxicity or other undesired effects. The amount of adenovirus cell surface receptors vary greatly among different cell types therefore the optimal concentration differs dramatically between cell types. A range of 10-200 MOI (multiplicity of infection) is used for most cell lines, but up to 1000 MOI may be used for lymphoid cell lines.Traditionally, Infectivity particles are measured in culture by a plaque-forming unit assay (PFU) that scores the number of viral plaques as a function of dilution. In contrast to the 10-day infection of a classical plaque assay, BioCrick's QuickTiter Adenovirus Titer Immunoassay Kit only requires 2-day infection, and there is no agar overlay step. The kit antibody against hexon protein recognizes all serotypes of adenovirus by immunocytochemistry (see Flow Chart). | ||
| Safety Considerations | Remember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood. | ||
| Dry Ice Shipment | Extra charge fee may add to your shipping cost as dry ice is required to ship this product. | ||
| Preparation and Storage | -80 degree C | ||
| Other Notes | Small volumes of Cdc42 N17 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Related Product Information for Cdc42 N17 recombinant protein | 1 x 10^11 viral particles/mL in TBS with 10% glycerolRecombinant adenoviruses have tremendous potential in both research and therapeutic applications. There are numerous advantages in using an adenovirus to introduce genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e. does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells.Three members of the Rho family small GTPase, Rho, Rac, and Cdc42, have been shown to play a crucial role in regulating the organization of the actin cytoskeleton in response to extracellular stimuli. Activation of Rho, Rac, and Cdc42 in quiescent Swiss 3T3 fibroblasts induces the assembly of filamentous actin into stress fibers, lamellipodia, and filopodia, respectively. In addition to these effects on the actin cytoskeleton, it has been shown Rac and Cdc42 (and in some cells Rho) can activate JNK and p38 that leads to transcriptional activation. In fibroblast cells, Rho, Rac, and Cdc42 have each been implicated in cell cycle control. The provided recombinant adenovirus contains dominant negative form of human Cdc42 (T17N). | ||
| Product Categories/Family for Cdc42 N17 recombinant protein | Viral Expression; Adenoviral Expression; Premade Recombinant Adenoviruses; Adenoviruses: Cytoskeleton Regulation / Small GTPase | ||
| Product References and Citations for Cdc42 N17 recombinant protein | 1. Bett AJ, Haddara W, Prevec L and Graham FL. (1994) Proc Natl Acad Sci U S A. 91:8802-6.2. Robbins, P. D., Tahara, H., and Ghivizzani, S. C. (1998) Trends Biotechnol. 16:35-40.3. Huang, S., Stupack, D., Mathias, P., Wang, Y., and Nemerow, G. (1997) Proc. Natl. Acad. Sci. U S A. 94:8156-8161.4. Bergelson, J. M., J. A. Cunningham, G. Droguett, E. A. Kurt-Jones, A. Krithivas, J. S. Hong, M. S. Horwitz, R. L. Crowell, and R. W. Finberg. (1997) Science 275:1320-1323.5. Machesky L. M. and Hall A. (1996) Trends Cell Biol. 6:304-10.1. Black, S.A. et al. (2008). TGFbeta1 stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGFbeta1-induced CCN2/CTGF expression. J. Biol. Chem. 283:10835-10847.2. Yu, W.-M. et al. (2009). Laminin is required for Schwann cell morphogenesis. J. Cell Sci. 122:929-936.3. Cheng, Z.-J. et al. (2010). Co-regulation of caveolar and Cdc42-dependent fluid phase endocytosis by phosphocaveolin-1. J. Biol. Chem. 285:15119-15125. | ||
| Precautions | All of BioCrick's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to BioCrick that you will properly test and use any Products purchased from BioCrick in accordance with industry standards. BioCrick and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. | ||
| Disclaimer | While every efforts were made to ensure the accuracy of the information provided in this datasheet, BioCrick will not be liable for any omissions or errors contained herein. BioCrick reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to BioCrick within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. | ||
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