The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C₁, C₂ & V₂ to calculate V₁.

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The dilution calculator equation

The dilution calculator is based on the following equation:

Concentration_(start) x Volume_(start) = Concentration_(final) x Volume_(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

An example of a dilution calculation using the dilution calculator

What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?

Using the equation C₁V₁ = C₂V₂, where C₁=10 mM, C₂=50 μM, V₂=10 ml and V₁ is the unknown:

1. Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
2. Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
3. Enter 20 into the Volume (final) box and select the correct unit (milliliter)
4. Press calculate
5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box