Fel d 1, Recombinant ProteinCatalog# BCP36806 |
| Product Name | Fel d 1, Recombinant Protein | ||
| Full Product Name | Recombinant allergen Fel d 1 for Felis domesticus (cat) | ||
| Product Gene Name | Fel d 1 recombinant protein | ||
| Research Use Only | For Research Use Only. Not for use in diagnostic procedures. | ||
| Host | Pichia pastoris | ||
| Purity/Purification | 15% SDS-PAGE | ||
| Form/Format | Dry powder (lyophilized); 20 mM HEPES buffer pH8, 0.15 M NaCl and 5 mM EDTA. | ||
| Organism | Epithelia | ||
| his-Fel d 1 | recombinant allergen with a his-tag | ||
| Quality Control | 1. Protein Concentration Determined EspectrophotometricallyDO280 = 0.877A0.1% (= g/l) = 0.593Concentration*: 1.48 mg/mlThe measurement of the protein concentration has been performed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 19892. Purity Control In SDS-PAGE: 15%3. Analysis By An ELISA AssayThe evaluation of the recombinant allergen has been evaluated in an external study carried out at a Spanish hospital by a group of allergists with positive and negative serum samples from patients. The evaluation of the recombinant allergens has been performed by means of an in-house ELISA assay.4. Absence of precipitation after a freezing and thawing cycle: Ok | ||
| Molecular Weight | Determined by SDS-PAGE, the protein shows three different bands which are between molecular markers of 25,000 and 18,400 Da, between 18,400 and 14,400 Da and below 14,400 Da while relative molecular mass calculated from amino acid sequence is 22,472.37 Da for the dimer and two bands of 13,000 and 10,000 Da for the monomeric chains. | ||
| Note | proteins should be maintained frozen at high concentrations. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface. | ||
| Preparation and Storage | Protein is shipped at room temperature. Upon arrival, it should be stored at 4 degree C or -20 degree C. Once reconstituted, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20 degree C to -80 degree C. | ||
| Other Notes | Small volumes of Fel d 1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Related Product Information for Fel d 1 recombinant protein | Recombinant Allergen: Fel d 1 is the major allergen of Felis domesticus (cat) (Ohman et al., 1976).Description: The Felis domesticus uteroglobin Fel d 1 has been prepared as the recombinant mature protein fused to a his-tag. | ||
| Application Notes for Fel d 1 recombinant protein | The evaluation of the recombinant allergen has been evaluated in an external study carried out at a Spanish hospital by a group of allergists with positive and negative serum samples from patients. The evaluation of the recombinant allergens has been performed by means of an in-house ELISA assay. In this immunoassay, it has been determined the presence of specific IgE in sera that had previously been validated by skin prick testing (SPT) and the UniCAP test. The sera panel for this study was composed of 25 positive and 10 negative specimen sera.The recombinant allergen Fel d 1 detected 22 positive sera out of 25 (82% incidence) with higher prevalence of sera with titers of 0.70-3.5 IU/ml (international units per milliliter; 1 IU is equivalent to 2.42 ng of IgE). | ||
| SDS-Page of Fel d 1 recombinant protein | Figure 1. SDS-PAGE analysis (15%) of 5 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis. | ||
| Product References and Citations for Fel d 1 recombinant protein | Ohman, J. L., Lowell, F. C., Bloch, K. J. and Kendall, S. Allergens of mammalian origin V. properties of extracts derived from domestic cat. 1976, Clin. Allergy 6:419-28.Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26. | ||
| Precautions | All of BioCrick's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to BioCrick that you will properly test and use any Products purchased from BioCrick in accordance with industry standards. BioCrick and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. | ||
| Disclaimer | While every efforts were made to ensure the accuracy of the information provided in this datasheet, BioCrick will not be liable for any omissions or errors contained herein. BioCrick reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to BioCrick within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. | ||
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