Sonic Hedgehog (Shh), Recombinant ProteinCatalog# BCP31966 |
| Product Name | Sonic Hedgehog (Shh), Recombinant Protein | ||
| Full Product Name | Recombinant Mouse Sonic Hedgehog Protein | ||
| Product Synonym Names | Sonic Hedgehog Protein; SHH; HHG-1; SHH | ||
| Product Gene Name | Shh recombinant protein | ||
| Research Use Only | For Research Use Only. Not for use in diagnostic procedures. | ||
| 3D Structure | ModBase 3D Structure for Q62226 | ||
| Host | E Coli | ||
| Purity/Purification | >95% as determined by reducing SDS-PAGE. | ||
| Form/Format | Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. | ||
| Species | Mouse | ||
| Endotoxin | <1.0 EU per mug as determined by LAL test. | ||
| Fragment | Met1-Gly198 | ||
| Activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.The ED50 for this effect is typically 5-40 mug/mL. | ||
| Preparation and Storage | Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.Aliquots of reconstituted samples are stable at < -20 degree C for 3 months. | ||
| ISO Certification | Manufactured in an ISO 9001:2015 Certified Laboratory. | ||
| Other Notes | Small volumes of Shh recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Related Product Information for Shh recombinant protein | Protein Construction: Recombinant Mouse Sonic Hedgehog is produced by our E Coli expression system and the target gene encoding Cys25-Gly198 is expressed.Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh-N, respectively. | ||
| NCBI GI # | 21617861 | ||
| NCBI GeneID | 20423 | ||
| NCBI Accession # | NP_033196.1 | ||
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