Anemhupehins A-C, Podocarpane Diterpenoids from Anemone hupehensis.[Pubmed: 29230718]

Three new podocarpane diterpenoids, namely anemhupehins A-C (1-3), together with four known analogues (4-7), have been isolated from aerial parts of Anemone hupehensis. Their structures were characterized based on extensive spectroscopic data. Compounds 1 and 4 showed certain cytotoxicities against human cancer cell lines.

Structure revision of hupehensis saponin F and G and characterization of new trace triterpenoid saponins from Anemone hupehensis by tandem electrospray ionization mass spectrometry.[Pubmed: 22507832]

Electrospray ionization ion-trap tandem mass spectrometry (ESI-MS(n)) was first employed for reinvestigating the structures of hupehensis saponin F and G previously isolated from Anemone hupehensis in our lab. Hupehensis saponin G was determined to contain one more trisaccharide unit (Rha-(1→4)-Glc-(1→6)-Glc-), not a glucose residue, than saponin F based on their molecular weights deduced from their [M+Na](+) ions in ESI-MS spectra. The (2,4)A(4α)-ion at m/z 551.3 formed by retro-Diels-Alder (RDA) rearrangement in positive mode illustrated that the C-28 sugar chains of the two saponins were composed of trisaccharide repeating moieties with (1→4) linkages rather than (1→3) linkages. The interpretation of 2D-NMR spectra of the two compounds also confirmed the results obtained by ESI-MS(n). Moreover, from the water soluble part of A. hupehensis, two novel triterpene saponins were tentatively characterized to contain 4 and 5 (1→4)-linked above trisaccharide repeating moieties at C-28 position according to their ESI-MS(n) behaviors, respectively.

Triterpene glycosides from the whole plant of Anemone hupehensis var. japonica and their cytotoxic activity.[Pubmed: 19952458]

Three new triterpene glycosides (1-3), together with eight known triterpene glycosides (4-11), were isolated from the whole plant of Anemone hupehensis var. japonica (Ranunculaceae). The structures of the new compounds were determined on the basis of spectroscopic analysis and the results of hydrolytic cleavage experiments. The isolated compounds were evaluated for their cytotoxic activities against HL-60 human leukemia cells, HSC-2 human oral squamous carcinoma cells, HSC-4 human oral squamous carcinoma cells, and A549 human lung adenocarcinoma cells.

Remarkable, overlooked and new microfungi in North Rhine-Westphalia, Germany.[Pubmed: 12425026]

During our investigations of the micro flora in NRW in the years of 1999 and 2000 we were able to collect and identify some new and rare species of micro fungi as parasites and saprophytes on wild and ornamental plants. Some of them are new for Germany: Podosphaera xanthii on Coreopsis verticillata; Cercospora traversiana on Trigonella foenum-graecum; Passalora dubia on Atriplex hortensis; Ophiobolus cirsii on Carduus spec.; Periconia britannica on Polemonium coeruleum; Ascochyta leptospora on Agropyron repens; Apomelasmia urticae on Urtica dioica; Cryptodiaporthe salicina on Salix caprea; Dasyscyphus nidulus on Anemone hupehensis; Rhopographus filicinus on Pteridium aquilinum; Sillia ferruginea on Corylus avellana; Sirococcus spiraeae on Spiraea spec. and Forsythia x intermedia. Examples of these findings are in the Herbarium ESS (Mycotheca Parva, Slg. Feige/Ale-Agha).

Triterpenoid saponins from Anemone hupehensis.[Pubmed: 9004545]

Two new triterpenoid saponins, named hupehensis saponin F and G, were isolated from the water soluble part of Anemone hupehensis Lemoine. By chemical and spectroscopic evidence, their structures were elucidated as 3-O-beta-D-ribopyranosyl(1-->3)-alpha-L-rhamnopyranosyl(1--> 2)-alpha-L-arabinopyranosyl hederagennin-28-O-alpha-rhamnopyranosyl(1--> 4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl(1--> 3)-alpha-L-rhamnopyranosyl(1-->4)-beta-glucopyranosyl(1--> 6)-beta-D-glucopyranoside and 3-O-beta-D-ribopyranosyl(1-->3)-alpha-L-rhamnopyranosyl(1--> 2)-alpha-L-arabinopyranosyl hederagenin-28-O-beta-glucopyranosyl(1-->3)-alpha-rhamnopyranosyl( 1--> 4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl(1--> 3)-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1--> 6)-beta-D-glucopyranoside, respectively.

Triterpenoid saponins from Anemone hupehensis.[Pubmed: 7764287]

Four triterpenoid saponins were isolated from Anemone hupehensis. On the basis of chemical and spectroscopic evidence, two new saponins, hupehensis saponins D and E, were elucidated as 3-O-beta-D-glucopyranosyl (1-->3)-beta-D-ribopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranosyl oleanolic acid-28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranoside and 3-O-beta-D-glucopyransoyl (1-->3-beta-D-ribopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranosyl hederagenin-28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranoside. The two known saponins were identified as huzhangosides B and D.

[Anti-HBsAg herbs employing ELISA technique].[Pubmed: 2268945]

With the aid of the ELISA system this schema represented a laboratory approach to the recognition of anti-HBsAg capability of herbs by using 300 herbal extracts. Altogether 10 herbs (3.0%) were identified as effective. When forming a multiplex plan by employing 10 average P/N ratios as exemplified by 5 varying doses of herb (0.3, 0.6, 1.2, 2.5, 5.0 mg/100 microliters), 2 varying concentrations of HBsAg (10.92, 14.26 P/N ratio), and 3 varying contact time periods (immediate, 1h, 2h) for the comprehensive appraisal of herb efficacy index, these 10 effective herbs were listed in the following order: Prunella vulgaris (1.00), Litchi chinensis (1.26), Gossypium herbaceum (1.45), Cudrania cochinochinensis (1.56), Caesalpinia sappan (1.73), Oldenlandia tenelliflora (1.77), Cautis parthenocissus (1.99), Evodia rutaecarpa (2.01), Portulaca grandiflora (2.44), and Anemone hupehensis (2.83).