The antioxidant activities effect of neutral and acidic polysaccharides from Epimedium acuminatum Franch. on Caenorhabditis elegans.[Pubmed: 27083801]

A neutral polysaccharide (EAP-1N) and an acidic polysaccharide (EAP-2A) were purified from Epimedium acuminatum by DEAE-52 cellulose anion-exchange chromatography and gel-filtration chromatography. Their structures were characterized by chemical composition analysis, high-performance size exclusion chromatography (HPSEC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Further, their antioxidant activities were investigated both in vitro and in vivo. Results showed that EAP-2A had higher uronic acid content and larger average molecular weight than EAP-1N. Compared with EAP-1N, EAP-2A exhibited significantly scavenging activities against free radical in vitro, as well as strongly stimulating effect on antioxidant enzyme activities (including superoxide dismutases (SOD), catalases (CAT), and glutathione peroxidases (GSH-PX)) and preferably inhibitory effect on lipid peroxidation and protein carboxyl in the mode of Caenorhabditis elegans.

Structural characterization of an acidic Epimedium polysaccharide and its immune-enhancement activity.[Pubmed: 26794746]

One acidic polysaccharide named EPS-1 was isolated from the aqueous extract of the leaves of Epimedium acuminatum Franch. It may be composed of 1,4-linked α-d-GalpA, 1,3,4-linked α-d-GalpA, 1,6-linked β-d-Galp and terminal α-l-Rhap residues in a molar ratio of 11.0:1.0:1.0:1.0 by chemical and spectroscopic analysis. EPS-1 possessed immune modulation effects on peripheral T lymphocyte and immature chBM-DCs such as promoting the proliferation and cytokine secretion of these cells and increasing the phagocytosis ability of immature chBM-DCs.

Extraction, antioxidant and antimicrobial activities of Epimedium acuminatum Franch. polysaccharide.[Pubmed: 23688459]

Polysaccharides from Epimedium acuminatum were extracted by hot water and optimized with response surface methodology. The optimal conditions of the extraction were determined to be the ratio of water to raw material of 29.61, extraction temperature of 85.67°C and extraction time of 3.57 h. Under these optimal conditions, the yield of polysaccharide was 8.21%, which was well matched with the predictive yield (8.23%). Moreover, three purified fractions (EAP40-1, EAP60-1 and EAP80-2) were obtained for further chemical analysis, antioxidant activity analysis and antimicrobial activity analysis. EAP40-1 with molecular weight of 138,884 Da showed the best radical scavenging activity. Meanwhile, EAP60-1 with molecular weight of 114,667 Da was found to exhibit significant antihemolytic activity and antimicrobial activity.

Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum.[Pubmed: 23218266]

The polysaccharides were extracted from Epimedium acuminatum by hot water extraction, ultrasonic-assisted extraction, enzyme extraction, and microwave-assisted extraction. The physicochemical properties of Epimedium polysaccharides were then determined by chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analysis. Further, the antioxidant activities were studied via different methods, including DPPH assay, ABTS assay, FRAP assay and AAPH-induced erythrocyte hemolysis assay. Results showed that the physicochemical properties of different polysaccharides were similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. The antioxidant activities of the polysaccharides which obtained by hot water extraction were higher than those of other polysaccharides. Overall, E. acuminatum polysaccharides might be used as potential natural antioxidant.

[Variation of icariin and total flavonoid of Epimedium acuminatum in different parts and habitats].[Pubmed: 23019870]

Through comparative study on contents of icariin and total flavonoid of Epimedium acuminatum in different habitats and parts, the distribution and correlation of the two components were observed.

[Study on modules biomass structure of Epimedium acuminatum in different habitats].[Pubmed: 21598532]

To study the biomass structure of Epimedium acuminatum Franch in the different ecological environments for the development and protection.