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trans-2-Hexen-1-al

CAS# 6728-26-3

trans-2-Hexen-1-al

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Quality Control of trans-2-Hexen-1-al

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Chemical structure

trans-2-Hexen-1-al

3D structure

Chemical Properties of trans-2-Hexen-1-al

Cas No. 6728-26-3 SDF Download SDF
PubChem ID 5281168 Appearance Oil
Formula C6H10O M.Wt 98.1
Type of Compound Miscellaneous Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (E)-hex-2-enal
SMILES CCCC=CC=O
Standard InChIKey MBDOYVRWFFCFHM-SNAWJCMRSA-N
Standard InChI InChI=1S/C6H10O/c1-2-3-4-5-6-7/h4-6H,2-3H2,1H3/b5-4+
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of trans-2-Hexen-1-al

The fruits of olive

Biological Activity of trans-2-Hexen-1-al

DescriptionTrans-2-hexen-1-al could used as a model molecule to screen for suitable sol-gel formulations.

trans-2-Hexen-1-al Dilution Calculator

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Preparing Stock Solutions of trans-2-Hexen-1-al

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 10.1937 mL 50.9684 mL 101.9368 mL 203.8736 mL 254.842 mL
5 mM 2.0387 mL 10.1937 mL 20.3874 mL 40.7747 mL 50.9684 mL
10 mM 1.0194 mL 5.0968 mL 10.1937 mL 20.3874 mL 25.4842 mL
50 mM 0.2039 mL 1.0194 mL 2.0387 mL 4.0775 mL 5.0968 mL
100 mM 0.1019 mL 0.5097 mL 1.0194 mL 2.0387 mL 2.5484 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on trans-2-Hexen-1-al

Understanding retention and metabolization of aroma compounds using an in vitro model of oral mucosa.[Pubmed:32126464]

Food Chem. 2020 Jul 15;318:126468.

The mechanism leading to aroma persistence during eating is not fully described. This study aims at better understanding the role of the oral mucosa in this phenomenon. Release of 14 volatile compounds from different chemical classes was studied after exposure to in vitro models of oral mucosa, at equilibrium by Gas-Chromatography-Flame Ionization Detection (GC-FID) and in dynamic conditions by Proton Transfer Reaction- Mass Spectrometry (PTR-MS). Measurements at equilibrium showed that mucosal hydration reduced the release of only two compounds, pentan-2-one and linalool (p < 0.05), and suggested that cells could metabolize aroma compounds from different chemical families (penta-2,3-dione, trans-2-Hexen-1-al, ethyl hexanoate, nonan- and decan-2-one). Dynamic analyses for pentan-2-one and octan-2-one evidenced that the constituents of the mucosal pellicle influenced release kinetics differently depending on molecule hydrophobicity. This work suggests that mucosal cells can metabolize aroma compounds and that non-covalent interactions occur between aroma compounds and oral mucosa depending on aroma chemical structure.

Biosensing with Insect Odorant Receptor Nanodiscs and Carbon Nanotube Field-Effect Transistors.[Pubmed:30740970]

ACS Appl Mater Interfaces. 2019 Mar 6;11(9):9530-9538.

Insect odorant receptors have been reconstituted into lipid nanodiscs and tethered to carbon nanotube field-effect transistors to function as a biosensor. Here, four different insect odorant receptors (ORs) from Drosophila melanogaster (DmelOR10a, DmelOR22a, DmelOR35a, and DmelOR71a) were expressed in Sf9 cells, purified, and reconstituted into lipid nanodiscs. We have demonstrated that each of these ORs produce a selective and highly sensitive electrical response to their respective positive ligands, methyl salicylate, methyl hexanoate, trans-2-Hexen-1-al, and 4-ethylguaiacol, with limits of detection in the low femtomolar range. No detection was observed for each OR against control ligands, and empty nanodiscs showed no specific sensor signal for any of the odorant molecules. Our results are the first evidence that insect ORs can be integrated into lipid nanodiscs and used as primary sensing elements for bioelectronic nose technologies.

Volatile compounds in different parts of the fruit Psidium guajava L. cv. "Media China" identified at distinct phenological stages using HS-SPME-GC-QTOF/MS.[Pubmed:30019400]

Phytochem Anal. 2018 Nov;29(6):649-660.

INTRODUCTION: Determination of the volatile organic compounds (VOCs) emitted by immature fruits furthers our understanding of plant-pest interactions and by fruits in a ripe state concerns food quality. OBJECTIVES: To apply headspace solid-phase microextraction gas chromatography quadrupole time-of-flight mass spectrometry (HS-SPME-GC-QTOF/MS) to compare the volatiles emitted by different parts of guava (Psidium guajava L. cv. "Media China") at different maturation stages. METHODOLOGY: HS-SPME combined with GC-QTOF/MS was used to characterise the VOCs of entire guavas in the orchard and under laboratory conditions. For chemical analysis X-ray fluorescence spectroscopy, refractometry, titration, complexometry, diode array detector high-performance liquid chromatography (DAD-HPLC) and refractive index detector (RI)-HPLC were used. RESULTS: The guava variety was rich in potassium and poor in sodium. A total of 44 VOCs were identified in different phenological stages and parts of the fruits. Release of VOCs was influenced by the temperature in the plantation, and transformation of innate fruit VOCs started immediately after cutting. CONCLUSION: The most abundant VOC released by the immature fruit in the plantation overnight was (S)-limonene, and it concentrated in the outer skin (pericarp). The esters ethyl benzoate, ethyl octanoate, butyl-2-methylbutanoate, ethyl hexanoate, cis-3-hexenyl acetate, and ethyl butanoate were emitted by ripe whole fruits. During ripening ethyl benzoate reached a maximum production after three to five days, while the formation of the aldehydes benzaldehyde, hexanal and trans-2-Hexen-1-al started thereafter.

Functional characterization of a Niemann-Pick type C2 protein in the parasitoid wasp Microplitis mediator.[Pubmed:28459128]

Insect Sci. 2018 Oct;25(5):765-777.

Niemann-Pick type C2 (NPC2) is a type of small soluble protein involved in lipid metabolism and triglyceride accumulation in vertebrates and arthropods. Recent studies have determined that NPC2 also participates in chemical communication of arthropods. In this work, two novel NPC2 proteins (MmedNPC2a and MmedNPC2b) in Microplitis mediator were identified. Real-time quantitative PCR (qPCR) analysis revealed that MmedNPC2a was expressed higher in the antennae than in other tissues of adult wasps compared with MmedNPC2b. Subsequent immunolocalization results demonstrated that NPC2a was located in the lymph cavities of sensilla placodea. To further explore the binding characterization of recombinant MmedNPC2a to 54 candidate odor molecules, a fluorescence binding assay was performed. It was found MmedNPC2a could not bind with selected fatty acids, such as linoleic acid, palmitic acid, stearic acid and octadecenoic acid. However, seven cotton volatiles, 4-ethylbenzaldehyde, 3,4-dimethylbenzaldehyde, beta-ionone, linalool, m-xylene, benzaldehyde and trans-2-Hexen-1-al showed certain binding abilities with MmedNPC2a. Moreover, the predicted 3D model of MmedNPC2a was composed of seven beta-sheets and three pairs of disulfide bridges. In this model, the key binding residues for oleic acid in CjapNPC2 of Camponotus japonicus, Lue68, Lys69, Lys70, Phe97, Thr103 and Phe127, are replaced with Phe85, Ser86, His87, Leu113, Tyr119 and Ile143 in MmedNPC2a, respectively. We proposed that MmedNPC2a in M. mediator may play roles in perception of plant volatiles.

Characterization of virgin olive oils produced with autochthonous Galician varieties.[Pubmed:27374520]

Food Chem. 2016 Dec 1;212:162-71.

The interest of Galician oil producers (NW Spain) in recovering the ancient autochthonous olive varieties Brava and Mansa has increased substantially in recent years. Virgin olive oils produced by co-crushing both varieties in two different proportions, reflecting the usual and most common practice adopted in this region, have gradually emerged for the production of virgin olive oils. Herein, the sensory and chemical characteristics of such oils were characterized by quality and genuineness-related parameters. The results of chemical analysis are discussed in terms of their effective contribution to the sensory profile, which suggests useful recommendations for olive oil producers to improve the quality of oils. Antioxidant compounds, together with aromas and coloured pigments were determined, and their contribution in determining the functional value and the sensory properties of oils was investigated. In general, given the high levels of phenolic compounds (ranging between 254 and 375mg/kg oil), tocopherols (about 165mg/kg oil) and carotenoids (10-12mg/kg oil); these are oils with long stability, especially under dark storage conditions, because stability is reinforced with the contribution of chlorophylls (15-22mg/kg oil). A major content of phenolic compounds, as well as a predominance of trans-2-Hexen-1-al within odor-active compounds (from 897 to 1645mug/kg oil), responsible for bitter sensory notes. This characterization allows to developing new antioxidant-rich and flavour-rich VOOs, when co-crushing with a higher proportion of Brava olives, satisfying the consumers' demand in having access to more healthy dishes and peculiar sensory attributes.

Slow Release of Plant Volatiles Using Sol-Gel Dispensers.[Pubmed:26470065]

J Econ Entomol. 2014 Dec;107(6):2023-9.

The black citrus aphid, also known as the tea aphid, (Toxoptera aurantii Boyer) attacks economically important crops, including tea (Camellia sinensis (L.) O. Kuntze). In the current study, silica sol-gel formulations were screened to find one that could carry and release C. sinensis plant volatiles to lure black citrus aphids in a greenhouse. The common plant volatile trans-2-Hexen-1-al was used as a model molecule to screen for suitable sol-gel formulations. A zNose (Electronic Sensor Technology, Newbury Park, CA) transportable gas chromatograph was used to continuously monitor the volatile emissions. A sol-gel formulation containing tetramethyl orthosilicate and methyltrimethoxysilane in an 8:2 (vol:vol) ratio was selected to develop a slow-release dispenser. The half-life of trans-2-Hexen-1-al in the sol-gel dispenser increased slightly with the volume of this compound in the dispenser. Ten different volatiles were tested in the sol-gel dispenser. Alcohols of 6-10 carbons had the longest half-lives (3.01-3.77 d), while esters of 6-12 carbons had the shortest (1.53-2.28 d). Release of these volatiles from the dispensers could not be detected by the zNose after 16 d (cis-3-hexenyl acetate) to 26 d (3,7-dimethylocta-1,6-dien-3-ol). In greenhouse experiments, trans-2-Hexen-1-al and cis-3-hexen-1-ol released from the sol-gel dispensers attracted aphids for asymptotically equal to17 d, and release of these volatiles could not be detected by the zNose after asymptotically equal to24 d. The sol-gel dispensers performed adequately for the slow release of plant volatiles to trap aphids in the greenhouse.

Aroma biogenesis and distribution between olive pulps and seeds with identification of aroma trends among cultivars.[Pubmed:23768404]

Food Chem. 2013 Nov 1;141(1):637-43.

The two constitutive parts of four cultivars (Arbequina, Picual, Local and Manzanilla de Sevilla) grown in Spain were separately analysed in order to establish the role of pulp and seed in the biogenesis of extra virgin olive oil (EVOO) aroma through the lipoxygenase (LOX) pathway. C6 and C5 volatile compounds responsible of EVOO aroma were produced by endogenous enzymes in both parts of olive fruits and the differences can be attributed to different enzymes distribution in pulp and seed. According to results, C6 and C5 volatile compounds have mainly their biogenesis in pulp (80-90%) vs. seed (20-10%), independently of the cultivar considered. A linear discriminant analysis was used to establish discriminant aroma compounds between pulp and seed related to the maturity index. A decrease in trans-2-Hexen-1-al and an increase in 1-hexanol with ripeness were observed independently of the cultivar considered. Finally, Partial Least Squares (PLS) regression analysis between pulp and seed aroma compounds allowed to establish those volatile compounds that better describe each cultivar.

Comparative characterization, expression pattern and function analysis of the 12-oxo-phytodienoic acid reductase gene family in rice.[Pubmed:21249367]

Plant Cell Rep. 2011 Jun;30(6):981-95.

The 12-oxo-phytodienoic acid reductases (OPRs) belong to the old yellow enzyme family of flavoenzymes and form multiple subfamilies in angiosperm plants. In our previous study, a comparative genomic analysis showed that five OPR subfamilies (subs. I-V) occur in monocots, and two subfamilies (subs. I and II) in dicots. Here, a comparative study of five OsOPR genes, representing five subfamilies (I-V) in rice, was performed to provide insights into OPR biochemical properties and physiological importance. Comparative analysis of the three-dimensional structure by homology modeling indicated all five OsOPR proteins contained a highly conserved backbone with (alpha/beta)(8)-barrels, while two middle variable regions (MVR i and ii) were also detected and defined. Analysis of enzymatic characteristics revealed that all five OsOPR fusion proteins exhibit distinct substrate specificity. Different catalytic activity was observed using racemic OPDA and trans-2-Hexen-1-al as substrates, suggesting OsOPR family genes participate in two main branches of the octadecanoid pathway, including the allene oxide synthase and hydroperoxide lyase pathways which regulate various developmental processes and/or defense responses. The transcript profiles of five OsOPR genes exhibited strong tissue-specific and inducible expression patterns under abiotic stress, hormones and plant wounding treatments. Furthermore, the transcriptions of OsOPR04-1 (OsOPR11) and OsOPR08-1 (OsOPR7), representing subs. I and II, respectively, were observed in all six selected tissues and with all above-stress treatments. This suggests that these two subfamilies play an important role during different developmental stages and in response to stresses; while the expressions of OsOPR06-1 (OsOPR6), OsOPR01-1 (OsOPR10) and OsOPR02-1 (OsOPR8), representing subs. III, IV and V respectively, were strongly up-regulated with abscisic acid (ABA) and indoleacetic acid (IAA) treatments in roots, suggesting these three subfamilies play an important role in responding to hormones especially ABA and IAA signals in roots.

Further field evaluation of synthetic herbivore-induced plant volatiles as attractants for beneficial insects.[Pubmed:15898496]

J Chem Ecol. 2005 Mar;31(3):481-95.

Fifteen synthetic herbivore-induced plant volatiles (HIPVs) were field-tested for attractivity to beneficial insects in two experiments conducted in an open field and a hop yard in Washington State. Eleven insect species or families showed significant attraction to 13 HIPVs. The ladybeetle, Stethorus punctum picipes, was attracted to sticky traps baited with methyl salicylate (MeSA), cis-3-hexen-1-ol (He), and benzaldehyde (Be). The minute pirate bug, Orius tristicolor, was attracted to traps baited with MeSA, He, Be, and octyl aldehyde (Oa), and the bigeyed bug, Geocoris pallens, responded to MeSA, indole, and trans-2-Hexen-1-al. The mymarid wasp, Anagrus daanei, was attracted to He, Oa, and farnesene. The chloropid fly, Thaumatomyia glabra, was highly attracted to methyl anthranilate. Insect families responding to HIPVs included Syrphidae (MeSA, He), Braconidae ((Z)-3-hexenyl acetate, He, cis-jasmone (J), methyl jasmonate (MeJA), methyl anthranilate (MeA)), Empididae (MeSA), Sarcophagidae (MeSA, Be, J, nonanal and geraniol), Tachinidae (Be), and Agromyzidae (MeSA). Micro-Hymenoptera (primarily parasitic wasp families) were attracted to MeSA, He, and indole. These results are discussed with respect to known properties and bioactivity of the tested HIPVs and to their potential as tools for recruiting natural enemies into agroecosystems.

Changes in the order of elution of some organic compounds in high-resolution gas chromatography on polar columns depending on the injection method and water content in the sample.[Pubmed:12735476]

J Chromatogr A. 2003 Apr 11;992(1-2):199-203.

During the GC analysis of apple aroma essence using a capillary column with polar stationary phases, i.e., bonded/crosslinked polyethylene glycol (HP-Innowax) and acid-modified polyethylene glycol (FFAP) the phenomenon of changing the elution order of some compounds was observed ("cross-over phenomenon"). The elution order within two pairs of closely eluting compounds: 2-methylbutyl acetate/pentan-3-ol (internal standard) and trans-2-Hexen-1-al/2-methylbutan-1-ol differed between samples introduced onto the column with direct injection and with static headspace method. Experiments carried out on standard solutions of investigated compounds in water-acetone mixtures of gradually reduced water ratio (99:1 to 0:100) proved that changes of elution times leading first to co-elution and subsequently to reversion of elution order of compounds within both pairs depended on the amount of water in the injected sample. The same effect was observed while the amount of water was diminished by reducing the sample volume introduced into the column.

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