Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic).[Pubmed: 30103674]

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Purified sulforaphane from broccoli (Brassica oleracea var. italica) leads to alterations of CDX1 and CDX2 expression and changes in miR-9 and miR-326 levels in human gastric cancer cells.[Pubmed: 30096452]

Genetic alterations and epigenetic modifications are two main factors involved in gastric carcinogenesis, progression, and metastasis. Several miRNAs such as miRNA-9 and miRNA-326 may play important role in gastric cancer by targeting the 3'UTR of the caudal type homeobox (CDX) 1 and 2 mRNA respectively. The use of herbal medicines has been widely considered in the treatment of cancers such as gastric cancer. Sulforaphane extracted from broccoli may indirectly prevent cancer through affecting different signaling pathways. The aim of this study was to evaluate the effect of different concentrations of sulforaphane extracted from broccoli sprout (SEBS) on viability, death pattern, and expression alterations of CDX1/2 as well as miRNA-9 and miRNA-326 in normal (HF2FF) and gastric cancer cell lines.

Human health risks from consuming cabbage (Brassica oleracea L. var. capitata) grown on wastewater irrigated soil.[Pubmed: 30095311]

The present study investigated the impact of cumulative irrigation with wastewater on the soil properties and, its health hazards on the consumers of cabbage plants at south Cairo Province, Egypt. Irrigation water, soil and cabbage plants were sample d from two polluted and other two unpolluted farms. The physicochemical properties of water and soil were analyzed and the growth parameters, as well as nutrients and heavy metals concentration in cabbage were investigated. In addition, the daily intake of metals (DIM) and health risk index (HRI) were estimated. Wastewater posed a decrease in the availability of N, P and K, but increases heavy metals in the soil solution. Cabbage stem and root lengths as well as the number of leaves and biomass were greatly reduced in the polluted farms. In addition, the photosynthetic pigments, carbohydrates and proteins were decreased under pollution stress. The concentration of most investigated metals in the leaves and roots were increased with translocation factor greater than one for Pb, Cd, As, Cr, Ni, Fe, and Co. The study revealed that the HRI exceeded one for Pb and Cd in polluted and unpolluted plants; and Fe in polluted ones. Irrigation with wastewater is not suitable for cabbage as it has health risks on humans due to accumulation of heavy metals. It worth noting that, the high ability of cabbage plants to accumulate Pb and Cd from both polluted and unpolluted soils should be taken into consideration when consuming this plant.

Polyphenolic composition, enzyme inhibitory effects ex-vivo and in-vivo studies on two Brassicaceae of north-central Italy.[Pubmed: 30086459]

In this study, three different extracts (soxhlet, microwave and decoction) from two species of broccoli: Brassica oleracea L. convar. Italica botrytis (L.) Alef. var. cymosa Duch. (Broccolo Fiolaro) and Brassica oleracea acephala L. convar. acephala (DC.) Alef. var. sabellica L. (Cavolo Nero), which are commonly spread in north-central Italy, were tested for their enzyme inhibitory effects. Enzyme inhibitory effects were investigated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The soxhlet extracts had the highest inhibitory AChE effects with 1.08 mgGALAE/g (in Cavolo Nero) and 0.90 mgGALAE/g (in Broccolo Fiolaro). The significant tyrosinase inhibitory effect was observed in the soxhlet extract of Cavolo Nero with 11.93 mgKAE/g. In addition, we evaluated the antioxidant activity of Broccolo Fiolaro and Cavolo Nero on lipopolysaccharide (LPS)-stimulated bladder, kidney and liver specimens, ex vivo. We observed a significant reduction of both nitrite and malondialdehyde (MDA) following treatment that indicates a significant inhibitory effect on oxidative/nitrosative stress and lipoperoxidation, respectively. Additionally, the blunting effect induced by extracts on LPS-induced lactate dehydrogenase (LDH) activity further support a protective effect by both Broccolo Fiolaro and Cavolo Nero in bladder, kidney and liver. HPLC analysis revealed that catechin, epicatechin, vanillic and 3-hydroxy benzoic acids were the major components. The phenolic components may contribute to the observed enzyme inhibitory effects. in vivo tests also demonstrated that the extracts decreased the biochemical parameters in diabetic rats. Particularly, we observed the reduction of plasma glucose levels, urea and total cholesterol following oral administration, with the higher inhibitory effects exerted by Broccolo Fiolaro compared to Cavolo Nero. Overall, our results could provide new insights on the use of these Broccoli species not only as foods but also as functional and nutraceutical supplements.

Maintenance of grafting-induced epigenetic variations in the asexual progeny of Brassica oleracea and B. juncea chimera.[Pubmed: 30086201]

Grafting-induced variations have been observed in many plant species, but the heritability of variation in progeny is not well understood. In our study, adventitious shoots from the C cell lineage of shoot apical meristem (SAM) grafting chimera TCC (where the origin of the outmost, middle and innermost cell layers, respectively, of SAM is designated by 'T' for tuber mustard and 'C' for red cabbage) were induced and identified as r-CCC (r = regenerated). To investigate the maintenance of grafting variations during cell propagation and regeneration, different generations of asexual progeny (r-CCCn, n = generation) were established through successive regeneration of axillary shoots from r-CCC. The fourth generation of r-CCC (r-CCC4) was selected to perform whole genome bisulfite sequencing for comparative analysis of hetero-grafting induced global methylation changes relative to r-s-CCC4 (s = self-grafting). Increased CHH methylation levels and proportions were observed in r-CCC4, with substantial changes occurring in the repeat elements. Small RNA sequencing revealed 1,135 specific small interfering RNA (siRNA) tags that were typically expressed in r-CCC, r-CCC2 and r-CCC4. Notably, 65% of these specific siRNAs were associated with repeat elements, termed RE siRNAs. Subsequent analysis revealed the CHH methylation of RE siRNA overlapping regions were mainly hypermethylated in r-CCC4, indicating they were responsible for directing and maintaining grafting-induced CHH methylation. Moreover, the expression of 13 differentially methylated genes (DMGs) correlated with the phenotypic variation, showing differential expression levels between r-CCC4 and r-s-CCC4. These DMGs were predominantly CG hypermethylated, their methylation modifications corresponded to the transcription of relative methyltransferase. This article is protected by copyright. All rights reserved.

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes.[Pubmed: 30083848]

Brassica oleracea var. botrytis, a very popular crop grown for its edible inflorescence, is bred only as a mutated annual cultivar and does not naturally occur in environment. Since cauliflower is still described as the most troublesome of all the B. oleracea vegetables regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic modifications. Here, we present a successful protocol for genetic transformation of cauliflower employing the process of agroinfection. The primary analysis of in vitro response of five cultivars allowed us to have chosen Pionier as the most promising cultivar; in consequence the Pionier was transformed via Rhizobium-mediated techniques in order to evaluate both, R. radiobacter (EHA 105, LBA 4404) and R. rhizogenes (ATCC 18534, A4) species. However, the latter system turned out to be more effective and, the A4 strain, in particular (72% transformation efficiency, 55% confirmed by GUS assay). That shows a promising technical advance especially when compared to the results of previous literature reports (e.g. 8.7% reported efficiency using R. rhizogenes). The transgenic cauliflower was obtained from hairy roots via organogenic callus induction. The potential transformants were analysed at the genomic and proteomic levels and their transgenic character was fully confirmed.

A study on the wetting properties of broccoli leaf surfaces and their time dependent self-healing after mechanical damage.[Pubmed: 30079427]

Plants are protected from the elements by a complex hierarchical epicuticular wax layer which has inspired the creation of super-hydrophobic and self-cleaning surfaces. Although many studies have been conducted on different plant wax systems to determine the mechanisms of water repulsion hardly any have studied the recovery of the epicuticular wax layer. In the current study the wetting properties and crystallographic nature of the wax surface of Brassica oleracea var. italica (broccoli) has been studied, as well as the time-dependent recovery of the surface after mechanical damage. It was found that the surface of the broccoli leaves is not only super-repulsive and self-cleaning in regards to water but also in regards to glycerol and formamide, both of which have considerably lower surface tension values. Furthermore, it was shown that the surface properties do indeed recover after damage and that this recovery is multi-stepped and strongly dependent on the recovery of the roughness of the surface.