5,7-Diacetoxyflavone

Journal of Membrane Science, 1997 , 125 (1) :177-187

Hydrolysis and regioselective transesterification catalyzed by immobilized lipases in membrane bioreactors[Reference: WebLink]

An experimental study of two types of immobilized lipase membrane reactors for the bioconversion of lipophilic substrates is reported.
METHODS AND RESULTS:
In one part of the work, the performance of biphasic membrane reactors has been investigated as a function of selected operating conditions for the hydrolysis of olive oil. In this type of reactor, aqueous and organic phases flow tangentially past the two surfaces of an enzyme containing membrane; intramembrane mass transfer is diffusive.Another part of the study refers to the regioselective transesterification of 5,7-Diacetoxyflavone in a monophasic non-aqueous membrane reactor wherein an organic phase solution of substrate is crossflow filtered through the wall of a tubular enzyme-loaded membrane; in this case, intramembrane mass transfer is convective.Immobilization of the enzyme both within the interior of the membranes and at their surfaces is considered.The influence of fluid dynamics parameters and immobilization site on the biphasic reactors performance has been studied using lipase from Candida rugosa.
CONCLUSIONS:
The effect of immobilization, of the amount of immobilized enzyme on the catalytic activity and regioselectivity efficiency in non aqueous system have been investigated using lipase from Pseudomonas cepacea.