Cevadine

CAS# 62-59-9

Cevadine

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Quality Control of Cevadine

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Chemical structure

Cevadine

3D structure

Chemical Properties of Cevadine

Cas No. 62-59-9 SDF Download SDF
PubChem ID 5380394 Appearance White powder
Formula C32H49NO9 M.Wt 591.7
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in chloroform, ethanol and methan
Chemical Name [(1R,2S,6S,9S,10R,11S,12S,14R,15S,18S,19S,22S,23S,25R)-1,10,11,12,14,23-hexahydroxy-6,10,19-trimethyl-24-oxa-4-azaheptacyclo[12.12.0.02,11.04,9.015,25.018,23.019,25]hexacosan-22-yl] (Z)-2-methylbut-2-enoate
SMILES CC=C(C)C(=O)OC1CCC2(C3C1(OC24CC5(C6CN7CC(CCC7C(C6(C(CC5(C4CC3)O)O)O)(C)O)C)O)O)C
Standard InChIKey DBUCFOVFALNEOO-HWBIYQLFSA-N
Standard InChI InChI=1S/C32H49NO9/c1-6-18(3)25(35)41-24-11-12-26(4)19-8-9-20-28(37)13-23(34)31(39)21(29(28,38)16-30(20,26)42-32(19,24)40)15-33-14-17(2)7-10-22(33)27(31,5)36/h6,17,19-24,34,36-40H,7-16H2,1-5H3/b18-6-/t17-,19-,20-,21-,22-,23-,24-,26-,27+,28+,29+,30+,31-,32-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Cevadine

1 Solanum sp. 2 Veratrum sp.

Biological Activity of Cevadine

DescriptionCevadine-induced changes of membrane potential and sodium transport of muscle membrane in a chloride-free solution.Membrane potential dependence of potential oscillation induced by cevadine in striated muscle.

Cevadine Dilution Calculator

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Cevadine Molarity Calculator

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Preparing Stock Solutions of Cevadine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.69 mL 8.4502 mL 16.9005 mL 33.8009 mL 42.2511 mL
5 mM 0.338 mL 1.69 mL 3.3801 mL 6.7602 mL 8.4502 mL
10 mM 0.169 mL 0.845 mL 1.69 mL 3.3801 mL 4.2251 mL
50 mM 0.0338 mL 0.169 mL 0.338 mL 0.676 mL 0.845 mL
100 mM 0.0169 mL 0.0845 mL 0.169 mL 0.338 mL 0.4225 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Cevadine

A highly sensitive quantification method for 12 plant toxins in human serum using liquid chromatography tandem mass spectrometry with a quick solid-phase extraction technique.[Pubmed:33099112]

J Pharm Biomed Anal. 2020 Oct 9;192:113676.

We developed a highly sensitive quantification method using liquid chromatography tandem mass spectrometry (LC/MS/MS) for 12 plant toxins in human serum. In this paper, we selected lycorine, galanthamine, protoveratrine A, protoveratrine B, veratramine, veratridine, jervine, cyclopamine, Cevadine, alpha-solanine, alpha-chaconine, and solanidine as targeted analytes. The ADME column was utilized for LC separation and a Monolithic SPE column (MonoSpin(R) C18) for analyte extraction. The total time for SPE clean-up and LC/MS/MS analysis was completed within 30 min. The method validation results were as follows: the linearity (r(2)) of each calibration curve was over 0.99; the inter- and intra-day accuracies were 92.7 %-116 % and 91.6 %-106 %, respectively; and the inter- and intra-day precisions were below 14 % and 11 %, respectively. Also, the lower limits of detection and quantification were 0.0071-0.15 and 0.022-0.46 ng/mL, respectively, indicating the method's high sensitivity. Finally, to confirm its feasibility, our method was applied to two model samples: (1) commercially available human serum and (2) pseudo poisoning serum via dilution of mouse serum with human serum. We were able to quantify alpha-chaconine at 0.84 +/- 0.02 ng/mL in the serum (Case 1) and protoveratrine A at 0.15 +/- 0.032 ng/mL in the pseudo poisoning serum (Case 2), demonstrating our method's practicality. This is the first time that the 12 plant toxins in human serum were simultaneously quantitated. Our method can investigate accidental poisonings involving toxic plants, enabling prompt decisions on patient treatment.

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