Caffeic acid tetramer

A new caffeic acid tetramer from the Dracocephalum moldavica L.[Pubmed:28805461]

Nat Prod Res. 2018 Feb;32(3):370-373.

A new Caffeic acid tetramer compound, named (+) methyl rabdosiin (4), together with seven known caffeic acid multimers (1-3, 5-8) and one caffeic acid monomer (9), were isolated from the aerial parts of Dracocephalum moldavica L. The structures of these compounds were assigned on the basis of 1D and 2D NMR spectroscopic and mass spectrometry analyses. The protective effects of compounds 2-4 against hydrogen peroxide (H(2)O(2))-induced apoptosis were evaluated in primary cardiomyocytes of SD neonatal rats in vitro by the MTT method. Three compounds exhibited potent protective activities at 12.5 mug/mL.

[Physicochemical stability and purification technology of caffeic acid tetramer from Arnebia euchroma].[Pubmed:18837312]

Zhongguo Zhong Yao Za Zhi. 2008 Jul;33(13):1552-5.

OBJECTIVE: To purify Caffeic acid tetramer (CAT) with macroporous resin on the basis of its fundamental physicochemical stability research. METHOD: The changes of CAT content were compared by HPLC method before and after the purification process, or while other conditions were altered. RESULT: LK001 was the best one among 7 kinds of macroporous resin in regard of purifying ability. The optimum absorbing technology was the solution concentration at 10 g x L(-1), pH at 4.5, and the flow rate at 3 BV x h(-1). The best eluting technology was 45% ethanol as eluting agent, pH at 5.0, eluting volume at 50 mL after applying super-purified water and 20% ethanol. The yield of product was 3. 6 percent, and the active compound CAT was 58 percent in the product. CONCLUSION: Macroporous resin LK001 is effective in enriching CAT from the crude extracts, thus this method of purification is advisable.

Biocatalytic properties of a peroxidase-active cell-free extract from onion solid wastes: caffeic acid oxidation.[Pubmed:18670892]

Biodegradation. 2009 Apr;20(2):143-53.

The exploitation of food residual sources consists of a major factor in reducing the polluting load of food industry wastes and developing novel added-value products. Plant food residues including trimmings and peels might contain a range of enzymes capable of transforming bio-organic molecules with potential phytotoxicity, including hydrolases, peroxidases and polyphenoloxidases. Although the use of bacterial and fungal enzymes has gained interest in studies pertaining to bioremediation applications, plant enzymes have been given less attention or even disregarded. In this view, this study aimed at the investigating the use of a crude peroxidase preparation from onion solid by-products for oxidising caffeic acid, a widespread o-diphenol, whose various derivatives may occur in food industry wastes, such as olive mill waste waters. Increased enzyme activity was observed at a pH value of 5, but considerable activity was also retained for pH up to 7. Favourable temperatures for increased activity varied between 20 degrees C and 40 degrees C, 30 degrees C being the optimal. Liquid chromatography-mass spectrometry analysis of a homogenate/H(2)O(2)-treated caffeic acid solution revealed the existence of a tetramer as major oxidation product. Based on the data generated, a putative pathway for the formation of the peroxidase-mediated Caffeic acid tetramer was proposed.