Cyclo(Ala-Phe)

CAS# 14474-78-3

Cyclo(Ala-Phe)

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Quality Control of Cyclo(Ala-Phe)

Number of papers citing our products

Chemical structure

Cyclo(Ala-Phe)

3D structure

Chemical Properties of Cyclo(Ala-Phe)

Cas No. 14474-78-3 SDF Download SDF
PubChem ID 139767 Appearance Powder
Formula C12H14N2O2 M.Wt 218.26
Type of Compound Miscellaneous Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 3-benzyl-6-methylpiperazine-2,5-dione
SMILES CC1C(=O)NC(C(=O)N1)CC2=CC=CC=C2
Standard InChIKey CNXWPOWVDIUTPS-UHFFFAOYSA-N
Standard InChI InChI=1S/C12H14N2O2/c1-8-11(15)14-10(12(16)13-8)7-9-5-3-2-4-6-9/h2-6,8,10H,7H2,1H3,(H,13,16)(H,14,15)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Cyclo(Ala-Phe)

The roots of Rubia schumanniana Pritzel

Biological Activity of Cyclo(Ala-Phe)

DescriptionCyclo(Phe-Ala) displays significant anti-diatom activity with the inhibitory rate of 50% at the concentration of 50 microg/mL.

Protocol of Cyclo(Ala-Phe)

Structure Identification
Wei Sheng Wu Xue Bao. 2013 Aug 4;53(8):825-31.

Anti-diatom compounds from marine bacterium Pseudomonas putida.[Pubmed: 24341274]

In order to provide more natural antifouling compounds, marine bacterium Pseudomonas putida isolated from the sponge Haliclona sp. was explored to test its anti-diatom compounds.
METHODS AND RESULTS:
The strain was identified by colonial morphology, scanning electron microscope (SEM) and 16S rDNA sequence analysis. The separation procedure was guided by bioactive (Anti-diatom) and chemical (TLC, DAD-HPLCand 1H NMR) analysis, and their structures were elucidated by spectrographic techniques. The anti-diatom activity of all purified compounds was assayed. Strain 272 isolated from the sponge Haliclona sp. was identified as Pseudomonas putida. Six diketopiperazine compounds were isolated from the culture of this strain and their structures were determined as cyclo(Leu-Pro) (1), cyclo (Leu-Ala) (2), cyclo(Phe-Ala) (Cyclo(Ala-Phe),3), cyclo(Val-Tyr) (4), cyclo(Ala-Tyr) (5), cyclo(Ala-Trp) (6); Compounds 3 and 6 displayed significant anti-diatom activity with the inhibitory rate of 50% and 85% at the concentration of 50 microg/mL, respectively.
CONCLUSIONS:
The anti-diatom compounds isolated from marine bacterium Pseudomonas putida were cyclo (Phe-Ala) and cyclo (Ala-Trp).

Cyclo(Ala-Phe) Dilution Calculator

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Cyclo(Ala-Phe) Molarity Calculator

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Preparing Stock Solutions of Cyclo(Ala-Phe)

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 4.5817 mL 22.9085 mL 45.8169 mL 91.6338 mL 114.5423 mL
5 mM 0.9163 mL 4.5817 mL 9.1634 mL 18.3268 mL 22.9085 mL
10 mM 0.4582 mL 2.2908 mL 4.5817 mL 9.1634 mL 11.4542 mL
50 mM 0.0916 mL 0.4582 mL 0.9163 mL 1.8327 mL 2.2908 mL
100 mM 0.0458 mL 0.2291 mL 0.4582 mL 0.9163 mL 1.1454 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Cyclo(Ala-Phe)

[Anti-diatom compounds from marine bacterium Pseudomonas putida].[Pubmed:24341274]

Wei Sheng Wu Xue Bao. 2013 Aug 4;53(8):825-31.

OBJECTIVE: In order to provide more natural antifouling compounds, marine bacterium Pseudomonas putida isolated from the sponge Haliclona sp. was explored to test its anti-diatom compounds. METHODS: The strain was identified by colonial morphology, scanning electron microscope (SEM) and 16S rDNA sequence analysis. The separation procedure was guided by bioactive (Anti-diatom) and chemical (TLC, DAD-HPLCand 1H NMR) analysis, and their structures were elucidated by spectrographic techniques. The anti-diatom activity of all purified compounds was assayed. RESULTS: Strain 272 isolated from the sponge Haliclona sp. was identified as Pseudomonas putida. Six diketopiperazine compounds were isolated from the culture of this strain and their structures were determined as cyclo(Leu-Pro) (1), cyclo (Leu-Ala) (2), cyclo(Phe-Ala) (3), cyclo(Val-Tyr) (4), cyclo(Ala-Tyr) (5), cyclo(Ala-Trp) (6); Compounds 3 and 6 displayed significant anti-diatom activity with the inhibitory rate of 50% and 85% at the concentration of 50 microg/mL, respectively. CONCLUSION: The anti-diatom compounds isolated from marine bacterium Pseudomonas putida were cyclo (Phe-Ala) and cyclo (Ala-Trp).

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