DL-Dithiothreitol

Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent as a reducing or deprotecting agent for thiolated DNA.

References:
[1]. Dithiothreitol, From Wikipedia

Suppression of GABA(B) receptor function in vivo by disulfide reducing agent, DL-dithiothreitol (DTT).[Pubmed:14760512]

Psychopharmacology (Berl). 2004 Jul;174(2):283-90.

RATIONALE: A recent in-vitro study demonstrated that the potent disulfide reducing agent, DL-Dithiothreitol (DTT), may alter the structural stability of the GABA(B) receptor, probably inactivating the disulfide bonds between four cysteine residues located in the GABA(B1(a)) receptor structure. OBJECTIVES: The present study was designed to evaluate whether DTT treatment was capable of antagonizing some behavioral effects of pharmacological stimulation of the GABA(B) receptor. METHODS: Experiments on sedation/hypnosis induced by the GABA(B) receptor agonists baclofen, SKF 97541, CGP 44532 and gamma-hydroxybutyric acid (GHB) in DBA mice and selectively bred GHB-sensitive (GHB-S) rats, and a GHB drug discrimination study in Long Evans rats were conducted. Specificity of the DTT action on the GABA(B) receptor was investigated by assessing its effect on the sedative/hypnotic effect induced by diazepam, ketamine and ethanol. RESULTS: DTT prevented the sedative/hypnotic effect of all GABA(B) receptor agonists tested and also reversed baclofen-induced sedation/hypnosis. In contrast, DTT had no effect on, or even potentiated, sedation/hypnosis produced by diazepam, ketamine or ethanol. DTT completely blocked the discriminative stimulus effects of GHB. CONCLUSIONS: These results are discussed in terms of DTT altering the stability of the binding domain of the GABA(B) receptor, hindering the drug-receptor interaction.

Accumulation and stripping behavior of silver ions at dl-dithiothreitol self-assembled monolayer modified gold electrodes.[Pubmed:18968934]

Talanta. 2003 Mar 1;59(3):501-7.

Silver ions can be entrapped at the DL-Dithiothreitol (HSCH(2)(CHOH)(2)CH(2)SH, DTT) self-assembled monolayer films modified gold electrodes. When the potential was made moving, an anodic peak was observed at about 0.23 V (vs. SCE). When the electrode Au/DTT was modified with dodecyl mercaptan further, more Ag (I) can be accumulated and the peak grows. Conditions, such as solution pH and supporting electrolyte, were optimized for Ag (I) determination. Under the selected conditions, i.e. 0.010 M pH 4.3 potassium hydrogen phthalate, preconcentration time of 5 min at open circuit, the anodic peak height is linear to the concentration of Ag (I) in the small range of 0.6-2.4 muM. The influence of some ions on the determination of Ag (I) was examined. The Br(-) ion makes the peak decrease and NCS(-) makes the peak increase. But the determination is not interfered by 1000-fold Pb(2+), Cd(2+), Hg(2+), Fe(3+), Ni(2+), Co(2+), Cu(2+) and Sn(2+) when EDTA was added into the solution. The mechanism involved was discussed.

Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent; as a reducing or "deprotecting" agent for thiolated DNA.

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