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6''-O-Acetylsaikosaponin D

CAS# 64340-45-0

6''-O-Acetylsaikosaponin D

Catalog No. BCN6392----Order now to get a substantial discount!

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6''-O-Acetylsaikosaponin D: 5mg $345 In Stock
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Chemical structure

6''-O-Acetylsaikosaponin D

3D structure

Chemical Properties of 6''-O-Acetylsaikosaponin D

Cas No. 64340-45-0 SDF Download SDF
PubChem ID 21637630 Appearance Powder
Formula C44H70O14 M.Wt 823.0
Type of Compound Triterpenoids Storage Desiccate at -20°C
Synonyms 6''-Acetylsaikosaponin d
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name [(2R,3S,4S,5R,6S)-6-[(2R,3R,4S,5S,6R)-3,5-dihydroxy-2-[[(1S,2R,4S,5R,8R,9R,10S,13S,14R,17S,18R)-2-hydroxy-9-(hydroxymethyl)-4,5,9,13,20,20-hexamethyl-24-oxahexacyclo[15.5.2.01,18.04,17.05,14.08,13]tetracos-15-en-10-yl]oxy]-6-methyloxan-4-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methyl acetate
SMILES CC1C(C(C(C(O1)OC2CCC3(C(C2(C)CO)CCC4(C3C=CC56C4(CC(C7(C5CC(CC7)(C)C)CO6)O)C)C)C)O)OC8C(C(C(C(O8)COC(=O)C)O)O)O)O
Standard InChIKey WDWZBAMDKXKRBA-YBZZVAQVSA-N
Standard InChI InChI=1S/C44H70O14/c1-22-30(48)35(58-36-33(51)32(50)31(49)24(56-36)19-53-23(2)46)34(52)37(55-22)57-29-11-12-39(5)25(40(29,6)20-45)9-13-41(7)26(39)10-14-44-27-17-38(3,4)15-16-43(27,21-54-44)28(47)18-42(41,44)8/h10,14,22,24-37,45,47-52H,9,11-13,15-21H2,1-8H3/t22-,24-,25-,26-,27-,28-,29+,30+,31-,32+,33-,34-,35+,36+,37+,39+,40+,41-,42+,43-,44+/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 6''-O-Acetylsaikosaponin D

The herb of Bupleurum chinense DC.

Protocol of 6''-O-Acetylsaikosaponin D

Structure Identification
Journal of China Pharmaceutical University, 2003.

Study on the Constituents of the Roots of Bupleurum marginatum.[Reference: WebLink]

To find out the bioactive principles of Bupleurum marginatum.
METHODS AND RESULTS:
By using normal chromatography and spectral evidences, compounds were isolated and elucidated from the roots of this plant. Thirteen compounds were isolated from this plant for the first time, whose structures were identified as (+)-anomalin(I), (+)-praeruptorin A(II), (+)3′- angeloyloxy-4′-keto-3′,4′-dihydroseselin(III), xylitol(IV), saikochromone A(V),6′-O-acetylsaikosaponin d(6-O-Acetylsaikosaponin d,VI), saikosaponin b 4(VII), saikosaponin a(VIII), saikosaponin d(IX), saikosaponin b 2(X), saikosaponin c(XI), β-sitosterol, and β-daucosterol, respectively.
CONCLUSIONS:
Among them, II and III were isolated from the genus for the first time.

6''-O-Acetylsaikosaponin D Dilution Calculator

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6''-O-Acetylsaikosaponin D Molarity Calculator

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Preparing Stock Solutions of 6''-O-Acetylsaikosaponin D

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.2151 mL 6.0753 mL 12.1507 mL 24.3013 mL 30.3767 mL
5 mM 0.243 mL 1.2151 mL 2.4301 mL 4.8603 mL 6.0753 mL
10 mM 0.1215 mL 0.6075 mL 1.2151 mL 2.4301 mL 3.0377 mL
50 mM 0.0243 mL 0.1215 mL 0.243 mL 0.486 mL 0.6075 mL
100 mM 0.0122 mL 0.0608 mL 0.1215 mL 0.243 mL 0.3038 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 6''-O-Acetylsaikosaponin D

Fast determination of saikosaponins in Bupleurum by rapid resolution liquid chromatography with evaporative light scattering detection.[Pubmed:19201128]

J Pharm Biomed Anal. 2009 May 1;49(4):1048-55.

A rapid resolution liquid chromatography coupled with evaporative light scattering detection method was established for simultaneous determination of six saikosaponins, namely saikosaponin a, saikosaponin c, saikosaponin d, 6''-O-acetylsaikosaponin a, 3''-O-acetylsaikosaponin d and 6''-O-acetylsaikosaponin d in Bupleurum. The analysis was performed by using an Agilent Zorbax SB-C18 column (1.8 microm, 3.0 mm x 50 mm i.d.) at gradient elution of water and acetonitrile, and the saikosaponins were well separated within 12 min, which provided about a fourfold reduction in analysis time by comparing a conventional high performance liquid chromatography method. Owing to their low ultraviolet absorption, the saikosaponins were detected by evaporative light scattering. The standard curves to quantify the saikosaponins were constructed by the log-log plot, which showed good linearity with the correlation coefficients exceeding 0.9954. The detection limits and quantification limits ranged in 8.38-25.00 microg/mL and 25.13-45.00 microg/mL, respectively. Satisfactory intra-day and inter-day precisions were achieved with the relative standard deviation (R.S.D.) less than 6.58%, and the average recoveries obtained were in the range of 96.9-100.4%. In addition, MeOH-1.0% (v/v) pyridine was found to be the best the extraction solvent when compared to MeOH and MeOH-1.0% (v/v) ammonia water. A total of 23 samples of roots of Bupleurum from different species or locations were examined with this analytical method, and their chemical profiles provided information for the chemotaxonomic investigation. The results demonstrated that the analytical method is highly effective for the quality evaluation of Bupleurum species.

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