Hordenine

CAS# 539-15-1

Hordenine

Catalog No. BCN1424----Order now to get a substantial discount!

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Quality Control of Hordenine

Number of papers citing our products

Chemical structure

Hordenine

3D structure

Chemical Properties of Hordenine

Cas No. 539-15-1 SDF Download SDF
PubChem ID 68313 Appearance Beige powder
Formula C10H15NO M.Wt 165.24
Type of Compound Alkaloids Storage Desiccate at -20°C
Synonyms Anhaline; Eremursine;3595-05-9;Peyocactine
Solubility DMSO : 250 mg/mL (1513.04 mM; Need ultrasonic)
Chemical Name 4-[2-(dimethylamino)ethyl]phenol
SMILES CN(C)CCC1=CC=C(C=C1)O
Standard InChIKey KUBCEEMXQZUPDQ-UHFFFAOYSA-N
Standard InChI InChI=1S/C10H15NO/c1-11(2)8-7-9-3-5-10(12)6-4-9/h3-6,12H,7-8H2,1-2H3
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Hordenine

The herbs of Dendrolobium triangulare

Biological Activity of Hordenine

DescriptionHordenine is an inhibitor of noradrenaline uptake, is also an effective inhibitor of hyperpigmentation. It can inhibit melanogenesis by suppressing cAMP production, which is involved in the expression of melanogenesis-related proteins. Hordenine also has a positive inotropic effect upon the heart, increases systolic and diastolic blood pressure, peripheral blood flow volume, inhibits gut movements.
TargetscAMP | TRPV | MAO
In vitro

Hordenine, a single compound produced during barley germination, inhibits melanogenesis in human melanocytes.[Pubmed: 23768344]

Food Chem. 2013 Nov 1;141(1):174-81.

Melanin plays an important role protecting skin against ultraviolet light injury. However, increased production and accumulation of melanin results in a large number of skin disorders.
METHODS AND RESULTS:
Here, we identified Hordenine as an active compound from germinated barley (Hordeum vulgare L.) and investigated the effects of Hordenine on melanogenesis and its mechanisms of action in human epidermal melanocytes. We measured melanin content, tyrosinase activity, expression of melanogenesis-related proteins, and cAMP production. Melanin content was significantly inhibited by Hordenine. The intracellular cAMP level was also reduced by Hordenine. In addition, expression of microphthalmia-associated transcription factor (MITF), an upstream transcription factor of tyrosinase as well as tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2, was inhibited by Hordenine.
CONCLUSIONS:
Taken together, these results show that Hordenine inhibited melanogenesis by suppressing cAMP production, which is involved in the expression of melanogenesis-related proteins and suggest that Hordenine may be an effective inhibitor of hyperpigmentation.

In vivo

[Pharmacological effects of hordenine].[Pubmed: 8582256]

Dtsch Tierarztl Wochenschr. 1995 Jun;102(6):228-32.

Hordenine is an ingredient of some plants which are used as feed for animals, i.e. in sprouting barley. After ingestion of such feed Hordenine may be detected in blood or urine of horses which in case of racing horses may be the facts of using prohibited compounds.
METHODS AND RESULTS:
Results of some experiments in pharmacological models show that Hordenine is an indirectly acting adrenergic drug. It liberates norepinephrine from stores. In isolated organs and those structures with reduced epinephrine contents the Hordenine-effect is only very poor. Experiments in intact animals (rats, dogs) show that Hordenine has a positive inotropic effect upon the heart, increases systolic and diastolic blood pressure, peripheral blood flow volume, inhibits gut movements but has no effect upon the psychomotorical behaviour of mice.
CONCLUSIONS:
All effects are short and only possible after high doses which are not to be expected after ingestion of Hordenine containing feed for horses. A measurable increase of the performance of racing horses is quite improbable.

Protocol of Hordenine

Kinase Assay

Deamination of hordenine by monoamine oxidase and its action on vasa deferentia of the rat.[Pubmed: 2570842]

J Pharm Pharmacol. 1989 Jun;41(6):421-3.

The selectivity of the naturally occurring amine, N,N-dimethyltyramine (Hordenine) for monoamine oxidase (MAO) and its action upon isolated vasa deferentia of the rat was investigated.
METHODS AND RESULTS:
Hordenine was deaminated by rat liver MAO with a Michaelis constant of 479 microM and maximum velocity of 128 nmol (mg protein)-1 h-1 compared with 144 microM and 482 nmol (mg protein)-1 h-1 for tyramine. Studies, with selective irreversible inhibitors of MAO, showed that Hordenine was a highly selective substrate for MAO-B of liver and that it was not deaminated by the MAO-A of intestinal epithelium. In contrast to tyramine, Hordenine did not produce contractions of isolated vasa deferentia. However, 25 microM Hordenine potentiated contractile responses of vasa, from control animals, to submaximal doses of noradrenaline and inhibited responses to tyramine. It did not alter responses, to noradrenaline, of vasa denervated by chronic pretreatment of rats with guanethidine. Therefore, it appears that Hordenine acted as an inhibitor of noradrenaline uptake, in isolated vasa deferentia. These results indicate that dietary-Hordenine is unlikely to be deaminated by intestinal MAO as this is predominantly MAO-A.
CONCLUSIONS:
Consequently, it is likely to be absorbed and could affect the sympathetic nervous system, by virtue of its action as an inhibitor of noradrenaline uptake.

Structure Identification
Biomed Chromatogr. 2014 Oct 30.

Selective extraction based on poly(MAA-VB-EGMDA) monolith followed by HPLC for determination of hordenine in plasma and urine samples.[Pubmed: 25355709]

Hordenine is an active compound found in several foods, herbs and beer.
METHODS AND RESULTS:
In this work, a novel sorbent was fabricated for selective solid-phase extraction (SPE) of Hordenine in biological samples. The organic polymer sorbent was synthesized in one step in the plastic barrel of a syringe by a pre-polymerization solution consisting of methacrylic acid (MAA), 4-vinylphenylboronic acid (VB) and ethylene glycol dimethacrylate (EGDMA). The conditions for preparation were optimized to generate a poly(MAA-VB-EGMDA) monolith with good permeability. The monolith exhibited good enrichment efficiency towards Hordenine. By using tyramine as the internal standard, a poly(MAA-VB-EGMDA)-based SPE-HPLC method was established for analysis of Hordenine. Conditions for SPE, including volume of eluting solvent, pH of sample solution, sampling rate and sample volume, were optimized. The proposed SPE-HPLC method presented good linearity (R2  = 0.9992) within 10-2000 ng/mL and the detection limits was 3 ng/mL, which is significantly more sensitive than reported methods.
CONCLUSIONS:
The method was also applied in plasma and urine samples; good capability of removing matrices was observed, while Hordenine in low content was well extracted and enriched. The recoveries were from 90.6 to 94.7% and from 89.3 to 91.5% for the spiked plasma and urine samples, respectively, with the relative standard deviations <4.7%.

Hordenine Dilution Calculator

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Hordenine Molarity Calculator

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Preparing Stock Solutions of Hordenine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 6.0518 mL 30.259 mL 60.518 mL 121.0361 mL 151.2951 mL
5 mM 1.2104 mL 6.0518 mL 12.1036 mL 24.2072 mL 30.259 mL
10 mM 0.6052 mL 3.0259 mL 6.0518 mL 12.1036 mL 15.1295 mL
50 mM 0.121 mL 0.6052 mL 1.2104 mL 2.4207 mL 3.0259 mL
100 mM 0.0605 mL 0.3026 mL 0.6052 mL 1.2104 mL 1.513 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Hordenine

Deamination of hordenine by monoamine oxidase and its action on vasa deferentia of the rat.[Pubmed:2570842]

J Pharm Pharmacol. 1989 Jun;41(6):421-3.

The selectivity of the naturally occurring amine, N,N-dimethyltyramine (Hordenine) for monoamine oxidase (MAO) and its action upon isolated vasa deferentia of the rat was investigated. Hordenine was deaminated by rat liver MAO with a Michaelis constant of 479 microM and maximum velocity of 128 nmol (mg protein)-1 h-1 compared with 144 microM and 482 nmol (mg protein)-1 h-1 for tyramine. Studies, with selective irreversible inhibitors of MAO, showed that Hordenine was a highly selective substrate for MAO-B of liver and that it was not deaminated by the MAO-A of intestinal epithelium. In contrast to tyramine, Hordenine did not produce contractions of isolated vasa deferentia. However, 25 microM Hordenine potentiated contractile responses of vasa, from control animals, to submaximal doses of noradrenaline and inhibited responses to tyramine. It did not alter responses, to noradrenaline, of vasa denervated by chronic pretreatment of rats with guanethidine. Therefore, it appears that Hordenine acted as an inhibitor of noradrenaline uptake, in isolated vasa deferentia. These results indicate that dietary-Hordenine is unlikely to be deaminated by intestinal MAO as this is predominantly MAO-A. Consequently, it is likely to be absorbed and could affect the sympathetic nervous system, by virtue of its action as an inhibitor of noradrenaline uptake.

Selective extraction based on poly(MAA-VB-EGMDA) monolith followed by HPLC for determination of hordenine in plasma and urine samples.[Pubmed:25355709]

Biomed Chromatogr. 2015 Jun;29(6):869-75.

Hordenine is an active compound found in several foods, herbs and beer. In this work, a novel sorbent was fabricated for selective solid-phase extraction (SPE) of Hordenine in biological samples. The organic polymer sorbent was synthesized in one step in the plastic barrel of a syringe by a pre-polymerization solution consisting of methacrylic acid (MAA), 4-vinylphenylboronic acid (VB) and ethylene glycol dimethacrylate (EGDMA). The conditions for preparation were optimized to generate a poly(MAA-VB-EGMDA) monolith with good permeability. The monolith exhibited good enrichment efficiency towards Hordenine. By using tyramine as the internal standard, a poly(MAA-VB-EGMDA)-based SPE-HPLC method was established for analysis of Hordenine. Conditions for SPE, including volume of eluting solvent, pH of sample solution, sampling rate and sample volume, were optimized. The proposed SPE-HPLC method presented good linearity (R(2) = 0.9992) within 10-2000 ng/mL and the detection limits was 3 ng/mL, which is significantly more sensitive than reported methods. The method was also applied in plasma and urine samples; good capability of removing matrices was observed, while Hordenine in low content was well extracted and enriched. The recoveries were from 90.6 to 94.7% and from 89.3 to 91.5% for the spiked plasma and urine samples, respectively, with the relative standard deviations <4.7%.

Hordenine, a single compound produced during barley germination, inhibits melanogenesis in human melanocytes.[Pubmed:23768344]

Food Chem. 2013 Nov 1;141(1):174-81.

Melanin plays an important role protecting skin against ultraviolet light injury. However, increased production and accumulation of melanin results in a large number of skin disorders. Here, we identified Hordenine as an active compound from germinated barley (Hordeum vulgare L.) and investigated the effects of Hordenine on melanogenesis and its mechanisms of action in human epidermal melanocytes. We measured melanin content, tyrosinase activity, expression of melanogenesis-related proteins, and cAMP production. Melanin content was significantly inhibited by Hordenine. The intracellular cAMP level was also reduced by Hordenine. In addition, expression of microphthalmia-associated transcription factor (MITF), an upstream transcription factor of tyrosinase as well as tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2, was inhibited by Hordenine. Taken together, these results show that Hordenine inhibited melanogenesis by suppressing cAMP production, which is involved in the expression of melanogenesis-related proteins and suggest that Hordenine may be an effective inhibitor of hyperpigmentation.

[Pharmacological effects of hordenine].[Pubmed:8582256]

Dtsch Tierarztl Wochenschr. 1995 Jun;102(6):228-32.

Hordenine is an ingredient of some plants which are used as feed for animals, i.e. in sprouting barley. After ingestion of such feed Hordenine may be detected in blood or urine of horses which in case of racing horses may be the facts of using prohibited compounds. Results of some experiments in pharmacological models show that Hordenine is an indirectly acting adrenergic drug. It liberates norepinephrine from stores. In isolated organs and those structures with reduced epinephrine contents the Hordenine-effect is only very poor. Experiments in intact animals (rats, dogs) show that Hordenine has a positive inotropic effect upon the heart, increases systolic and diastolic blood pressure, peripheral blood flow volume, inhibits gut movements but has no effect upon the psychomotorical behaviour of mice. All effects are short and only possible after high doses which are not to be expected after ingestion of Hordenine containing feed for horses. A measurable increase of the performance of racing horses is quite improbable.

Description

Hordenine, an alkaloid found in plants, inhibits melanogenesis by suppression of cyclic adenosine monophosphate (cAMP) production.

Keywords:

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