Tombozine

Chemistry of Natural Compounds,2000 ,36 (5) :540-540

Alkaloids of Vinca minor[Reference: WebLink]

from the aerial part of the plant (3).
METHODS AND RESULTS:
We investigated alkaloids of the aerial part of V. minor cultivated in the Tashkent Botanical Garden during flowering. The aerial part (0.105 kg) was collected in June, 1997, wetted with aqueous ammonia (8%), left for 2 h, and extracted seven times with CHCl . Alkaloids were extracted from the condensed CHCl extracts by H SO (10%). The acid extract was 33 2 4 basicified with NaOH (10%) and extracted with CHCl . Then the alkaline solution was treated with ammonium chloride. The 3 phenolic part was extracted with CHCl . 3 The nonphenolic part (CHCl ) yielded 0.53 g; the phenolic part, 0.12 g. The overall yield was 0.65 g or 0.64% of the 3 dry weight. Column chromatography on silica gel of the nonphenolic part afforded vinc amine (3, 4), akuammicine (3, 5), reserpine (3, 6), majdine (3, 6), reserpinine (3, 6), vinerine (3, 6), ervine (3, 6), vineridine (3, 6), Tombozine (3, 6), vincamajine (3 , 6), and vincanine (3, 6). Column chromatography on silica gel of the phenolic part gave vincanidine (3, 6).
CONCLUSIONS:
All iso lated bases were identified by direct comparison of mixed melting points and IR and UV spectra with authentic samples. The last nine alkaloids were isolated for the first time from the examined species.