Bellendine

CAS# 32152-73-1

Bellendine

2D Structure

Catalog No. BCN1895----Order now to get a substantial discount!

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3D structure

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Bellendine

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Chemical Properties of Bellendine

Cas No. 32152-73-1 SDF Download SDF
PubChem ID 442996 Appearance Needles
Formula C12H15NO2 M.Wt 205.26
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
SMILES CC1=COC2=C(C1=O)C3CCC(C2)N3C
Standard InChIKey ODQUOOUDMCGJCX-UHFFFAOYSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Protocol of Bellendine

Structure Identification
Australian Journal of Chemistry,1979,32(8):1827-1840.

Alkaloids of Bellendena montana[Reference: WebLink]


METHODS AND RESULTS:
Fourteen alkaloids have been isolated from Bellendena montana,a monotypic proteaceous plant endemic in Tasmania. The structures of ten of these, Bellendine (1), isoBellendine (2), darlingine (3),5,11-dihydroisoBellendine(5), 2,3-dihydroBellendine (6), 2,3-epidihydroBellendine (7), 2,3-dihydrodarlingine(8), 3α-acetoxy-6β-isobutoxytropane (9), 6β-acetoxy-3α-isobutoxytropane (10) and 3α-acetoxy-6β-hydroxytropane(11), have been established.

Phytochemistry,1971,10(2):475-477.

Methyl (p-hydroxybenzoyl) acetate and an alkaloid, bellendine, from Bellendena montana[Reference: WebLink]


METHODS AND RESULTS:
The previously unreported methyl (p-hydroxybenzoyl) acetate, and the first alkaloids from the Proteaceae, have been isolated from the flowers of Bellendena montana. The major alkaloidal constituent, Bellendine, was obtained crystalline and characterized spectroscopically.

Bellendine Dilution Calculator

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Bellendine Molarity Calculator

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Preparing Stock Solutions of Bellendine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 4.8719 mL 24.3593 mL 48.7187 mL 97.4374 mL 121.7967 mL
5 mM 0.9744 mL 4.8719 mL 9.7437 mL 19.4875 mL 24.3593 mL
10 mM 0.4872 mL 2.4359 mL 4.8719 mL 9.7437 mL 12.1797 mL
50 mM 0.0974 mL 0.4872 mL 0.9744 mL 1.9487 mL 2.4359 mL
100 mM 0.0487 mL 0.2436 mL 0.4872 mL 0.9744 mL 1.218 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Bellendine

Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water.[Pubmed:30830325]

Metabolomics. 2017 Dec 4;14(1):11.

INTRODUCTION: Active microorganisms have been recently discovered in clouds, thus demonstrating the capacity of microorganisms to exist in harsh environments, including exposure to UV and oxidants, osmotic and cold shocks, etc. It is important to understand how microorganisms respond to and survive such stresses at the metabolic level. OBJECTIVES: The objective of this work is to assess metabolome modulation in a strain of Pseudomonas syringae isolated from cloud water and facing temperature downshift from 17 to 5 degrees C by identifying key molecules and pathways of the response/adaptation to cold shock. METHODS: Bacterial extracts from suspensions of cells grown at 17 degrees C and further incubated in microcosms at 5 and 17 degrees C to mimic cloud conditions were analysed by combining LC-MS and NMR; the results were evaluated in comparison to similar suspensions kept at constant temperature. The differences in the metabolome profiles were deciphered using multivariate statistics (PLS-DA). RESULTS: Key cold shock biomarkers were observed, including cryoprotectants (trehalose, glucose, glycerol, carnitine, glutamate), antioxidants (glutathione and carnitine) and their precursors, alkaloids (Bellendine and slaframine) and metabolites involved in energy metabolism (ATP, carbohydrates). Furthermore, new short peptides (nine dipeptides and a tetrapeptide) were found that have no known function. CONCLUSIONS: This study shows that in response to cold temperatures, Pseudomonas syringae PDD-32b-74 demonstrates numerous metabolism modifications to counteract the impacts of low temperatures.

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