Gosferol

Antiviral and cytotoxic evaluation of coumarins from Prangos ferulacea.[Pubmed:25026335]

Pharm Biol. 2014 Dec;52(12):1543-9.

CONTEXT: Prangos ferulacea (L.) Lindl. (Apiaceae) is a perennial plant found in the Middle-East, where it is commonly used as an antispasmodic and anti-inflammatory agent. It is a rich source of coumarins. OBJECTIVE: To purify several coumarins from P. ferulacea and to screen their cytotoxicity and anti-herpes activity. MATERIALS AND METHODS: Acetone extract of roots of P. ferulacea was subjected to several chromatographic separations to render pure coumarins (1-8). Anti-herpes virus effects of 1-7 were evaluated at concentration 2.5, 5, and 10 microgmL(-1), on a confluent monolayer of Vero cells infected with 25 PFU of HSV1. Cytotoxic effects of 1 and 2 were evaluated on an A2780S cell line using the MTT assay. The cells were exposed to a series of concentrations of coumarins (0.01-2.5 mM, 37 degrees C, 72 h). RESULTS: Compounds 1-8 were identified as osthole, isoimperatorin, oxypeucedanin, psoralen, oxypeucedanin hydrate, Gosferol, oxypeucedanin methnolate, and pranferol. This is the first report of occurrence of 4 and 7 in this plant. Compound 1 showed a viability of 9.41% +/- 2.4 at 2.5 mM on A2780S cells (IC50 = 0.38 mM). The cell survival of 2 at 2.5 mM was 46.86% +/- 5.5 with IC50 equal to 1.1 mM. DISCUSSION AND CONCLUSION: Compound 1 shows cytotoxic effects on the A2780S cell line. Compound 2 is a cyclooxygenase-2 inhibitor and the A2780S cell line does not express COX-2 which may interpret the non-toxic effect of the compound on this cell line. None of the tested compounds showed an anti-HSV effect at non-toxic concentrations.

[Quantitative analysis of five components in the rhizome of Angelica polymorpha by RP-HPLC under different UV wavelengths].[Pubmed:21174769]

Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(19):2581-4.

A HPLC method was developed for simultaneouly quantitative analyses of aviprin (1), Gosferol (2), 3'R-(+)-hamaudol (3), 3'-O-acetylhamaudol (4) and iso-imperatorin (5) in the rhizome of Angelica polymorpha. The analysis was performed at 25 degrees C on an Agilent Eclipse XDB-C18 analytical column (4.6 mm x 250 mm, 5 microm) with the mobile phase of methanol and H2O in gradient elution [55:45 (0 min)-65:35 (25 min)-95:5 (35 min)]. And the flow rate was 1.0 mL min(-1) with the detection wavelengths of 312 nm (1), 306 nm (2), 300 nm (3), 294 nm (4) and 310 nm (5). Consequently, the reqresion equations were Y = 1.81 x 10(3) X + 7.93 x 10(2) (r = 0.9996), Y = 2.49 x 10(3) X - 2.17 x 10(2) (r = 0.9993), Y = 2.02 x 10(3) X - 1.42 x 10(2) (r = 0.9991), Y = 1.57 x 10(3) X - 0.66 x 10(2) (r = 0.999 7), Y = 2.65 x 10(3) X - 1.47 x 10(2) (r = 0.999 6). And the average recoveries were 99.7% (RSD 0.57%), 100.1% (RSD 1.3%), 100.0% (RSD 1.6%), 99.6% (RSD 1.3%), 99.2% (RSD 0.59%), respectively. The precision, repeatability and stability were all consistent with the request of quantitative analysis. The contents of compound 1-5 in A. polymorpha were determined as 0.525%, 0.044%, 0.046%, 0.043%, 0.15%, respectively. Accordingly, this quantitative analysis method is good for the quality control of A. polymorpha.

Two new coumarin glucosides from the roots of Angelica apaensis and their anti-platelet aggregation activity.[Pubmed:17703728]

Arch Pharm Res. 2007 Jul;30(7):799-802.

Two new coumarin glucosides, 11-O-beta-D-glucopyranosyl thamnosmonin (1) and 12-O-beta-D-glucopyranosyl Gosferol (2), were isolated from the roots of Angelica apaensis. Their structures were elucidated spectroscopically. Both compounds showed weak inhibitory effects on rabbit platelet aggregation induced by PAF, AA and APD.

Minor furocoumarins of Murraya koenigii.[Pubmed:10844176]

Fitoterapia. 2000 Jun;71(3):334-7.

Xanthotoxin, isobyakangelicol, phellopterin, Gosferol, neobyakangelicol, byakangelicol, byakangelicin and isoGosferol are reported as minor furocoumarins of Murraya koenigii seeds.