HEPES

CAS# 7365-45-9

HEPES

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Product Name & Size Price Stock
HEPES: 5mg $6 In Stock
HEPES: 10mg Please Inquire In Stock
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Quality Control of HEPES

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Chemical structure

HEPES

3D structure

Chemical Properties of HEPES

Cas No. 7365-45-9 SDF Download SDF
PubChem ID 23831 Appearance Powder
Formula C8H18N2O4S M.Wt 238.3
Type of Compound N/A Storage Desiccate at -20°C
Solubility Soluble to 1000 mM in water
Chemical Name 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
SMILES C1CN(CCN1CCO)CCS(=O)(=O)O
Standard InChIKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
Standard InChI InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of HEPES

DescriptionMulti purpose buffer used in biological research.

HEPES Dilution Calculator

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HEPES Molarity Calculator

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Preparing Stock Solutions of HEPES

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 4.1964 mL 20.982 mL 41.9639 mL 83.9278 mL 104.9098 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL 16.7856 mL 20.982 mL
10 mM 0.4196 mL 2.0982 mL 4.1964 mL 8.3928 mL 10.491 mL
50 mM 0.0839 mL 0.4196 mL 0.8393 mL 1.6786 mL 2.0982 mL
100 mM 0.042 mL 0.2098 mL 0.4196 mL 0.8393 mL 1.0491 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on HEPES

Interaction of HEPES buffer with glass-ceramic scaffold: Can HEPES replace TRIS in SBF?[Pubmed:27889932]

J Biomed Mater Res B Appl Biomater. 2018 Jan;106(1):143-152.

An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good s buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES. The tested scaffold (45S5 bioactive glass-based) was exposed to the media under a static-dynamic arrangement (solutions were replaced on a daily basis) for 15 days. Leachate samples were collected daily for the analysis of Ca(2+) ions and Si (AAS), (PO4 )(3-) ions (UV-VIS), and to measure pH. The glass-ceramic scaffold was analyzed by SEM/EDS, XRD, and WD-XRF before and after 0.3, 1, 3, 7, 11, and 15 days of exposure. Our results confirmed the rapid selective dissolution of the glass-ceramic crystalline phase (Combeite) containing Ca(2+) ions due to the presence of HEPES, hydroxyapatite supersaturation being reached within 24 h in both solutions. These new results suggest that, like TRIS, HEPES buffer is not suitable for the in vitro testing of highly reactive inorganic biomaterials (glass, glass-ceramics). The ISO standard for such tests requires revision, but HEPES is not a viable alternative to TRIS buffer. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 143-152, 2018.

Hydrophilic interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer.[Pubmed:27993432]

J Pharm Biomed Anal. 2017 Feb 20;135:227-233.

HEPES is a zwitterionic buffer component used as a raw material in the GMP-manufacturing of advanced therapy medicinal products (ATMPs), hence requiring an adequate assay method with sufficient selectivity toward related impurities. Therefore, a hydrophilic interaction chromatography (HILIC) method was developed. Different factors were investigated towards the retention behavior of HEPES, its analogue EPPS and its starting material isethionate: pH, ion concentration and organic solvent ratio of the mobile phase, as well as column temperature. Moreover, stress testing resulted in the N-oxide degradant, identified by high resolution MS. The final method consisted of an isocratic system with an aqueous (pH 2.0 with H3PO4) acetonitrile (35:65, v/v) mobile phase on a zwitterionic HILIC (Obelisc N) column with a flow rate of 0.5mL/min and UV detection at 195nm. The assay method of HEPES was validated, obtaining adequate linearity (R(2)=0.999), precision (RSD of 0.5%) and accuracy (recovery of 100.08%). Finally, the applicability of the validated method was demonstrated by analysis of samples from different suppliers.

Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.[Pubmed:27060444]

J Microbiol Methods. 2016 Jun;125:40-2.

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.

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