Prunetin

CAS# 552-59-0

Prunetin

Catalog No. BCN2335----Order now to get a substantial discount!

Product Name & Size Price Stock
Prunetin: 5mg $86 In Stock
Prunetin: 10mg Please Inquire In Stock
Prunetin: 20mg Please Inquire Please Inquire
Prunetin: 50mg Please Inquire Please Inquire
Prunetin: 100mg Please Inquire Please Inquire
Prunetin: 200mg Please Inquire Please Inquire
Prunetin: 500mg Please Inquire Please Inquire
Prunetin: 1000mg Please Inquire Please Inquire

Quality Control of Prunetin

Number of papers citing our products

Chemical structure

Prunetin

3D structure

Chemical Properties of Prunetin

Cas No. 552-59-0 SDF Download SDF
PubChem ID 5281804 Appearance Powder
Formula C16H12O5 M.Wt 284.26
Type of Compound Flavonoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 5-hydroxy-3-(4-hydroxyphenyl)-7-methoxychromen-4-one
SMILES COC1=CC(=C2C(=C1)OC=C(C2=O)C3=CC=C(C=C3)O)O
Standard InChIKey KQMVAGISDHMXJJ-UHFFFAOYSA-N
Standard InChI InChI=1S/C16H12O5/c1-20-11-6-13(18)15-14(7-11)21-8-12(16(15)19)9-2-4-10(17)5-3-9/h2-8,17-18H,1H3
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Prunetin

The herbs of Millettia dielsiana

Biological Activity of Prunetin

DescriptionPrunetin mediates anti-obesity/adipogenesis effects by suppressing obesity-related transcription through a feedback mechanism that regulates the expression of adiponectin, adipoR1, adipoR2, and AMPK. Prunetin and biochanin A are potent reducers of NF-κB and ERK activation, zonula occludens 1 tyrosine phosphorylation, and metalloproteinase-mediated shedding activity, which may account for the barrier-improving ability of these isoflavones.
TargetsNOS | COX | TNF-α | IL Receptor | NF-kB | PGE | IkB | p65 | ERK | PPAR | HMG-CoA Reductase | AMPK | EGFR | IKK
In vitro

Effect of Prunetin on TNF-α-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IκB and Translocation of NF-κB p65 in Human Airway Epithelial Cells.[Pubmed: 24348668]

Tuberc Respir Dis (Seoul). 2013 Nov;75(5):205-9.

We investigated whether Prunetin significantly affects tumor necrosis factor-α (TNF-α)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IκB) degradation and nuclear factor kappa B (NF-κB) p65 translocation in human airway epithelial cells.
METHODS AND RESULTS:
Confluent NCI-H292 cells were pretreated with Prunetin for 30 minutes and then stimulated with TNF-α for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of Prunetin on TNF-α-induced degradation of IκB and translocation of NF-κB p65 was investigated by western blot analysis. We found that incubation of NCI-H292 cells with Prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-α. Prunetin inhibited TNF-α-induced degradation of IκB and translocation of NF-κB p65.
CONCLUSIONS:
This result suggests that Prunetin inhibits the NF-κB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-κB signaling pathway.

Biochanin A and prunetin improve epithelial barrier function in intestinal CaCo-2 cells via downregulation of ERK, NF-κB, and tyrosine phosphorylation.[Pubmed: 24631489]

Free Radic Biol Med. 2014 May;70:255-64.

The single-layered gut epithelium represents the primary line of defense against environmental stressors; thereby monolayer integrity and tightness are essentially required to maintain gut health and function. To date only a few plant-derived phytochemicals have been described as affecting intestinal barrier function.
CONCLUSIONS:
We investigated the impact of 28 secondary plant compounds on the barrier function of intestinal epithelial CaCo-2/TC-7 cells via transepithelial electrical resistance (TEER) measurements. Apart from genistein, the compounds that had the biggest effect in the TEER measurements were biochanin A and Prunetin. These isoflavones improved barrier tightness by 36 and 60%, respectively, compared to the untreated control. Furthermore, both isoflavones significantly attenuated TNFα-dependent barrier disruption, thereby maintaining a high barrier resistance comparable to nonstressed cells. In docking analyses exploring the putative interaction with the tyrosine kinase EGFR, these novel modulators of barrier tightness showed very similar values compared to the known tyrosine kinase inhibitor genistein.
CONCLUSIONS:
Both biochanin A and Prunetin were also identified as potent reducers of NF-κB and ERK activation, zonula occludens 1 tyrosine phosphorylation, and metalloproteinase-mediated shedding activity, which may account for the barrier-improving ability of these isoflavones.

Protocol of Prunetin

Kinase Assay

Anti-inflammatory effect of prunetin via the suppression of NF-κB pathway.[Pubmed: 23597450]

Food Chem Toxicol. 2013 Aug;58:124-32.

Prunetin is an O-methylated isoflavone, which is found in Prunus yedoensis. To date no report has been published on anti-inflammatory activities of Prunetin.
METHODS AND RESULTS:
In the present study, the anti-inflammatory effect of Prunetin on LPS-stimulated RAW 264.7 macrophage and LPS-induced septic shock model were investigated. Inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) expressions were determined by western blot and or realtime-PCR (RT-PCR). To elucidate its underlying mechanism, nuclear factor-kappa B (NF-κb) activation and its downstream pathways were investigated by NF-κB transcription factor assay, reporter gene expression, and western blot. In vivo anti-inflammatory effects of Prunetin were evaluated in LPS-induced endotoxemia. Promoter assay revealed that Prunetin inhibits LPS-induced nitric oxide and prostaglandin E2 production through the suppression of iNOS and COX-2 at the transcriptional level. In addition, Prunetin inhibits NF-κB-dependent inflammatory responses by modulating IκB kinase (IKK)-inhibitor κBα (IκBα)-NF-κB signaling. Consistent with these results, Prunetin significantly reduced serum levels of inflammatory cytokines and mortality in mice challenged with lipopolysaccharide.
CONCLUSIONS:
These findings offer a potential mechanism for the anti-inflammatory activity of Prunetin.

Cell Research

Inhibition of secretion, production and gene expression of mucin from cultured airway epithelial cells by prunetin.[Pubmed: 21305630]

Phytother Res. 2011 Aug;25(8):1196-200.

This study investigated whether Prunetin significantly affects the secretion, production and gene expression of mucin from cultured airway epithelial cells.
METHODS AND RESULTS:
Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then chased for 30 min in the presence of Prunetin to assess the effect on mucin secretion using enzyme-linked immunosorbent assay (ELISA). At the same time, confluent NCI-H292 cells were pretreated with Prunetin for 30 min and then stimulated with epidermal growth factor (EGF) or phorbol 12-myristate 13-acetate (PMA) for 24 h, respectively. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and ELISA. The results were as follows: (1) Prunetin significantly suppressed ATP-induced mucin secretion from cultured RTSE cells; (2) Prunetin inhibited the production of MUC5AC mucin protein induced by EGF or PMA from NCI-H292 cells; (3) Prunetin also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA from NCI-H292 cells.
CONCLUSIONS:
This result suggests that Prunetin can regulate the secretion, production and gene expression of mucin, by directly acting on airway epithelial cells.

Animal Research

Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone.[Pubmed: 23438470]

Biochem Pharmacol. 2013 May 15;85(10):1525-33.

Prunetin is an O-methylated isoflavone, which is a type of flavonoid. There are a limited number of reports detailing the biological activities of Prunetin. Although an anti-inflammatory effect of Prunetin has been reported in vitro, to our knowledge, there have been no reports on anti-adipogenic effects of Prunetin in obese animals.
METHODS AND RESULTS:
The aims of this study were to determine whether Prunetin suppresses high-fat diet (HFD)-induced adipogenesis in the liver and visceral adipose tissues of mice, and to explore the underlying mechanisms mediating the actions of Prunetin. To this end, mice were fed a HFD for 10 weeks to induce obesity, and Prunetin (10 μg/kg or 20 μg/kg) was administered in the last 3 weeks. Compared to saline-treated mice, mice treated with Prunetin showed significantly reduced body weight gain, visceral fat pad weights, and plasma glucose levels. We found that Prunetin significantly inhibited the HFD-induced upregulation of the expression of important adipogenic genes (PPARγ, C/EBPα, SREBP, aP2, LPL adiponectin, and leptin), and suppressed HFD-mediated increase in expression of lipid metabolism-related genes (SREBP, PPARγ, LXR, and HMG-CoA) in the liver tissues. Furthermore, Prunetin induced expression of adiponectin receptors 1 and 2 (adipoR1, adipoR2), as well as that of AMP-activated protein kinase (AMPK) in the liver and adipose tissue.
CONCLUSIONS:
These results suggest that Prunetin mediates anti-obesity/adipogenesis effects by suppressing obesity-related transcription through a feedback mechanism that regulates the expression of adiponectin, adipoR1, adipoR2, and AMPK.

Prunetin Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Prunetin Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Prunetin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.5179 mL 17.5895 mL 35.1791 mL 70.3581 mL 87.9477 mL
5 mM 0.7036 mL 3.5179 mL 7.0358 mL 14.0716 mL 17.5895 mL
10 mM 0.3518 mL 1.759 mL 3.5179 mL 7.0358 mL 8.7948 mL
50 mM 0.0704 mL 0.3518 mL 0.7036 mL 1.4072 mL 1.759 mL
100 mM 0.0352 mL 0.1759 mL 0.3518 mL 0.7036 mL 0.8795 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University
Featured Products
New Products
 

References on Prunetin

Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone.[Pubmed:23438470]

Biochem Pharmacol. 2013 May 15;85(10):1525-33.

Prunetin is an O-methylated isoflavone, which is a type of flavonoid. There are a limited number of reports detailing the biological activities of Prunetin. Although an anti-inflammatory effect of Prunetin has been reported in vitro, to our knowledge, there have been no reports on anti-adipogenic effects of Prunetin in obese animals. The aims of this study were to determine whether Prunetin suppresses high-fat diet (HFD)-induced adipogenesis in the liver and visceral adipose tissues of mice, and to explore the underlying mechanisms mediating the actions of Prunetin. To this end, mice were fed a HFD for 10 weeks to induce obesity, and Prunetin (10 mug/kg or 20 mug/kg) was administered in the last 3 weeks. Compared to saline-treated mice, mice treated with Prunetin showed significantly reduced body weight gain, visceral fat pad weights, and plasma glucose levels. We found that Prunetin significantly inhibited the HFD-induced upregulation of the expression of important adipogenic genes (PPARgamma, C/EBPalpha, SREBP, aP2, LPL adiponectin, and leptin), and suppressed HFD-mediated increase in expression of lipid metabolism-related genes (SREBP, PPARgamma, LXR, and HMG-CoA) in the liver tissues. Furthermore, Prunetin induced expression of adiponectin receptors 1 and 2 (adipoR1, adipoR2), as well as that of AMP-activated protein kinase (AMPK) in the liver and adipose tissue. These results suggest that Prunetin mediates anti-obesity/adipogenesis effects by suppressing obesity-related transcription through a feedback mechanism that regulates the expression of adiponectin, adipoR1, adipoR2, and AMPK.

Inhibition of secretion, production and gene expression of mucin from cultured airway epithelial cells by prunetin.[Pubmed:21305630]

Phytother Res. 2011 Aug;25(8):1196-200.

This study investigated whether Prunetin significantly affects the secretion, production and gene expression of mucin from cultured airway epithelial cells. Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then chased for 30 min in the presence of Prunetin to assess the effect on mucin secretion using enzyme-linked immunosorbent assay (ELISA). At the same time, confluent NCI-H292 cells were pretreated with Prunetin for 30 min and then stimulated with epidermal growth factor (EGF) or phorbol 12-myristate 13-acetate (PMA) for 24 h, respectively. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and ELISA. The results were as follows: (1) Prunetin significantly suppressed ATP-induced mucin secretion from cultured RTSE cells; (2) Prunetin inhibited the production of MUC5AC mucin protein induced by EGF or PMA from NCI-H292 cells; (3) Prunetin also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA from NCI-H292 cells. This result suggests that Prunetin can regulate the secretion, production and gene expression of mucin, by directly acting on airway epithelial cells.

Anti-inflammatory effect of prunetin via the suppression of NF-kappaB pathway.[Pubmed:23597450]

Food Chem Toxicol. 2013 Aug;58:124-32.

Prunetin is an O-methylated isoflavone, which is found in Prunus yedoensis. To date no report has been published on anti-inflammatory activities of Prunetin. In the present study, the anti-inflammatory effect of Prunetin on LPS-stimulated RAW 264.7 macrophage and LPS-induced septic shock model were investigated. Inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1beta (IL-1beta) expressions were determined by western blot and or realtime-PCR (RT-PCR). To elucidate its underlying mechanism, nuclear factor-kappa B (NF-kappab) activation and its downstream pathways were investigated by NF-kappaB transcription factor assay, reporter gene expression, and western blot. In vivo anti-inflammatory effects of Prunetin were evaluated in LPS-induced endotoxemia. Promoter assay revealed that Prunetin inhibits LPS-induced nitric oxide and prostaglandin E2 production through the suppression of iNOS and COX-2 at the transcriptional level. In addition, Prunetin inhibits NF-kappaB-dependent inflammatory responses by modulating IkappaB kinase (IKK)-inhibitor kappaBalpha (IkappaBalpha)-NF-kappaB signaling. Consistent with these results, Prunetin significantly reduced serum levels of inflammatory cytokines and mortality in mice challenged with lipopolysaccharide. These findings offer a potential mechanism for the anti-inflammatory activity of Prunetin.

Effect of Prunetin on TNF-alpha-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IkappaB and Translocation of NF-kappaB p65 in Human Airway Epithelial Cells.[Pubmed:24348668]

Tuberc Respir Dis (Seoul). 2013 Nov;75(5):205-9.

BACKGROUND: We investigated whether Prunetin significantly affects tumor necrosis factor-alpha (TNF-alpha)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IkappaB) degradation and nuclear factor kappa B (NF-kappaB) p65 translocation in human airway epithelial cells. METHODS: Confluent NCI-H292 cells were pretreated with Prunetin for 30 minutes and then stimulated with TNF-alpha for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of Prunetin on TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65 was investigated by western blot analysis. RESULTS: We found that incubation of NCI-H292 cells with Prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-alpha. Prunetin inhibited TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65. CONCLUSION: This result suggests that Prunetin inhibits the NF-kappaB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-kappaB signaling pathway.

Biochanin A and prunetin improve epithelial barrier function in intestinal CaCo-2 cells via downregulation of ERK, NF-kappaB, and tyrosine phosphorylation.[Pubmed:24631489]

Free Radic Biol Med. 2014 May;70:255-64.

The single-layered gut epithelium represents the primary line of defense against environmental stressors; thereby monolayer integrity and tightness are essentially required to maintain gut health and function. To date only a few plant-derived phytochemicals have been described as affecting intestinal barrier function. We investigated the impact of 28 secondary plant compounds on the barrier function of intestinal epithelial CaCo-2/TC-7 cells via transepithelial electrical resistance (TEER) measurements. Apart from genistein, the compounds that had the biggest effect in the TEER measurements were biochanin A and Prunetin. These isoflavones improved barrier tightness by 36 and 60%, respectively, compared to the untreated control. Furthermore, both isoflavones significantly attenuated TNFalpha-dependent barrier disruption, thereby maintaining a high barrier resistance comparable to nonstressed cells. In docking analyses exploring the putative interaction with the tyrosine kinase EGFR, these novel modulators of barrier tightness showed very similar values compared to the known tyrosine kinase inhibitor genistein. Both biochanin A and Prunetin were also identified as potent reducers of NF-kappaB and ERK activation, zonula occludens 1 tyrosine phosphorylation, and metalloproteinase-mediated shedding activity, which may account for the barrier-improving ability of these isoflavones.

Description

Prunetin, an O-methylated isoflavone, possesses anti-inflammatory activity. Prunetin is a potent human aldehyde dehydrogenases inhibitor.

Keywords:

Prunetin,552-59-0,Natural Products, buy Prunetin , Prunetin supplier , purchase Prunetin , Prunetin cost , Prunetin manufacturer , order Prunetin , high purity Prunetin

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: