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Rehmannioside C

CAS# 81720-07-2

Rehmannioside C

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Quality Control of Rehmannioside C

Number of papers citing our products

Chemical structure

Rehmannioside C

3D structure

Chemical Properties of Rehmannioside C

Cas No. 81720-07-2 SDF Download SDF
PubChem ID 125181686 Appearance Powder
Formula C21H34O14 M.Wt 510.49
Type of Compound Iridoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (2S,3R,4S,5S,6R)-2-[[(1S,4aR,5R,7S,7aS)-5-hydroxy-7-methyl-7-[(2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-1-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES CC1(CC(C2C1C(OC=C2)OC3C(C(C(C(O3)CO)O)O)O)O)OC4C(C(C(C(O4)CO)O)O)O
Standard InChIKey MITBZAODGSBUIS-YSCABPIUSA-N
Standard InChI InChI=1S/C21H34O14/c1-21(35-20-17(30)15(28)13(26)10(6-23)33-20)4-8(24)7-2-3-31-18(11(7)21)34-19-16(29)14(27)12(25)9(5-22)32-19/h2-3,7-20,22-30H,4-6H2,1H3/t7-,8+,9+,10+,11+,12+,13-,14-,15-,16+,17+,18-,19-,20+,21-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Rehmannioside C

The roots of Rehmannia glutinosa

Biological Activity of Rehmannioside C

In vitro

HPLC/ESI-MS analysis of the Radix Rehmanniae Praeparata.[Reference: WebLink]

Chinese Journal of Pharmaceutical Analysis, 2007,27:874-876.

To develop a high performance liquid chromatography-electrospray ionization-mass spec- trometry(HPLC-ESI-MS)analysis method of Radix Rehmanniae Praeparata sample.
METHODS AND RESULTS:
HPLC conditions were as follows:Zorbax SB-C_(18)(250 mm×4.6 mm,5 μm)column;gradient elution with acetonitrile-water;flow rate was 1 mL·min~(-1);photodiode-array detector(DAD)set at 210 nm;the analysis time was 50 min.MS condi- tions were as follows:Agilent ion trap mass spectrometer;ESI ion source;negative ionization mode.Catal- pol,dihydrocatalpol,leonuride,aucubin,rehmannioside A,rehmannioside B,Rehmannioside C and rehmannioside D in Radix Rehmanniae Praeparata sample were identified by MS~2.The MS~2fragments of the 8 constituents were ana- lyzed.
CONCLUSIONS:
In the negative ion mass detection mode 8 compounds can be simultaneously analyzed which provides a quality control method for Radix Rehmanniae Praeparata sample.

Rehmannioside C Dilution Calculator

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Preparing Stock Solutions of Rehmannioside C

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.9589 mL 9.7945 mL 19.589 mL 39.178 mL 48.9726 mL
5 mM 0.3918 mL 1.9589 mL 3.9178 mL 7.8356 mL 9.7945 mL
10 mM 0.1959 mL 0.9795 mL 1.9589 mL 3.9178 mL 4.8973 mL
50 mM 0.0392 mL 0.1959 mL 0.3918 mL 0.7836 mL 0.9795 mL
100 mM 0.0196 mL 0.0979 mL 0.1959 mL 0.3918 mL 0.4897 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Rehmannioside C

[Bio-activity evaluation of Qinlian Siwu decoction on inhibiting mice uterine contraction in vitro and its components analysis].[Pubmed:21438408]

Zhongguo Zhong Yao Za Zhi. 2010 Dec;35(24):3362-7.

OBJECTIVE: To evaluate the bio-activity of Qinlian Siwu decoction on in vitro uterus contraction model and exploit the relationship between chemical components and the bio-activity. METHOD: The samples were prepared by macroporous adsorptive resins. The in vitro uterus contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. RESULT: It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the contraction of the mouse uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, Rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. CONCLUSION: In vitro inhibiting the contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.

Description

Rehmannioside C is an iridoid glucoside isolated from Radix Rehmanniae Praeparata.

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