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3,5-Dihydroxybenzaldehyde

CAS# 26153-38-8

3,5-Dihydroxybenzaldehyde

2D Structure

Catalog No. BCN2257----Order now to get a substantial discount!

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3D structure

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3,5-Dihydroxybenzaldehyde

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Chemical Properties of 3,5-Dihydroxybenzaldehyde

Cas No. 26153-38-8 SDF Download SDF
PubChem ID 94365 Appearance Powder
Formula C7H6O3 M.Wt 138.12
Type of Compound Phenols Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 3,5-dihydroxybenzaldehyde
SMILES C1=C(C=C(C=C1O)O)C=O
Standard InChIKey HAQLHRYUDBKTJG-UHFFFAOYSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 3,5-Dihydroxybenzaldehyde

The herbs of Ranunculus ternatus

Protocol of 3,5-Dihydroxybenzaldehyde

Structure Identification
Rapid Commun Mass Spectrom, 2010, 24(5):634-642.

Liquid chromatographic/electrospray ionization mass spectrometric identification of the oxidation end-products of trans -resveratrol in aqueous solutions.[Reference: WebLink]

trans-Resveratrol (3,5,4'-trihydroxystilbene) is a natural polyphenolic compound that exhibits antioxidant properties. Our study aimed at studying the HO*-induced oxidation of resveratrol (100 micromol.L(-1)) in aerated aqueous solutions.
METHODS AND RESULTS:
Gamma radiolysis of water was used to generate HO*/O(2)(*-) free radicals (I = 10 Gy.min(-1), dose = 400 Gy). Oxidation products were identified by direct infusion mass spectrometry and high-performance liquid chromatography/mass spectrometry. For each product, structural elucidation was based on simple mass spectra, fragmentation spectra and deuterium/hydrogen exchange spectra; the comparison with mass spectra of synthetic products provided valuable information allowing the complete identification of the oxidation products.
CONCLUSIONS:
Four products resulting from the direct attack of HO* radicals towards resveratrol were identified respectively as piceatannol (trans-3,5,3',4'-tetrahydroxystilbene), 3,5-dihydroxybenzoic acid, 3,5-Dihydroxybenzaldehyde and 4-hydroxybenzaldehyde.

Indian Journal of Pharmaceutical Sciences,73,1(2011-11-11), 2011, 73(1):46-56.

Stability-indicating HPLC Method for Simultaneous Determination of Terbutaline Sulphate, Bromhexine Hydrochloride and Guaifenesin.[Reference: WebLink]

The aim of the present study was the development and subsequent validation of a simple, precise and stability-indicating reversed phase HPLC method for the simultaneous determination of guaifenesin, terbutaline sulphate and bromhexine hydrochloride in the presence of their potential impurities in a single run.
METHODS AND RESULTS:
The photolytic as well as hydrolytic impurities were detected as 3,5-dihydroxybenzoic acid, 3,5-Dihydroxybenzaldehyde, 1-(3,5-dihydroxyphenyl)-2-[(1,1-dimethylethyl) amino]-ethanone from terbutaline, 2-methoxyphenol and an unknown impurity identified as (2RS)-3-(2-hydroxyphenoxy)-propane-1,2-diol from guaifenesin. The chromatographic separation of all the three active components and their impurities was achieved on Wakosil II column, using phosphate buffer (pH 3.0) and acetonitrile as mobile phase which was delivered initially in the ratio of 80:20 (v/v) for 18 min, then changed to 60:40 (v/v) for next 12 min, and finally equilibrated back to 80:20 (v/v) for 10 min. Other HPLC parameters were: Flow rate at 1.0 ml/min, detection wavelengths 248 and 280 nm, injection volume 10 μl. The calibration graphs plotted with five concentrations of each component were linear with a regression coefficient R(2) >0.9999. The limit of detection and limit of quantitation were estimated for all the five impurities. The established method was then validated for linearity, precision, accuracy, and specificity and demonstrated to be applicable to the determination of the active ingredients in commercial and model cough syrup. No interference from the formulation excipients was observed.
CONCLUSIONS:
These results suggest that this LC method can be used for the determination of multiple active ingredients and their impurities in a cough and cold syrup.

3,5-Dihydroxybenzaldehyde Dilution Calculator

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3,5-Dihydroxybenzaldehyde Molarity Calculator

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Preparing Stock Solutions of 3,5-Dihydroxybenzaldehyde

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 7.2401 mL 36.2004 mL 72.4008 mL 144.8016 mL 181.002 mL
5 mM 1.448 mL 7.2401 mL 14.4802 mL 28.9603 mL 36.2004 mL
10 mM 0.724 mL 3.62 mL 7.2401 mL 14.4802 mL 18.1002 mL
50 mM 0.1448 mL 0.724 mL 1.448 mL 2.896 mL 3.62 mL
100 mM 0.0724 mL 0.362 mL 0.724 mL 1.448 mL 1.81 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 3,5-Dihydroxybenzaldehyde

Photo-protection by 3-bromo-4, 5-dihydroxybenzaldehyde against ultraviolet B-induced oxidative stress in human keratinocytes.[Pubmed:22795593]

Ecotoxicol Environ Saf. 2012 Sep;83:71-8.

Exposure of the skin to ultraviolet B (UVB) radiation leads to epidermal damage and the generation of reactive oxygen species (ROS) in skin cells, including keratinocytes. Therefore, the photo-protective effect of 3-bromo-4, 5-dihydroxybenzaldehyde (BDB) against UVB was assessed in human HaCaT keratinocytes exposed to UVB radiation in vitro. BDB restored cell viability, which decreased upon exposure to UVB radiation. BDB exhibited scavenging activity against 1, 1-diphenyl-2-picrylhydrazyl radicals, intracellular ROS induced by hydrogen peroxide (H(2)O(2)) or UVB radiation, the superoxide anion generated by the xanthine/xanthine oxidase system, and the hydroxyl radical generated by the Fenton reaction (FeSO(4)+H(2)O(2)). Moreover, BDB absorbed UVB and decreased injury resulting from UVB-induced oxidative stress to lipids, proteins and DNA. Finally, BDB reduced UVB-induced apoptosis, as exemplified by fewer apoptotic bodies and a reduction in DNA fragmentation. Taken together, these results suggest that BDB protects human keratinocytes against UVB-induced oxidative stress by scavenging ROS and absorbing UVB rays, thereby reducing injury to cellular components.

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