4-O-MethylbuteinCAS# 13323-67-6 |
Quality Control & MSDS
3D structure
Package In Stock
Number of papers citing our products
| Cas No. | 13323-67-6 | SDF | Download SDF |
| PubChem ID | 5372365 | Appearance | Yellow powder |
| Formula | C16H14O5 | M.Wt | 286.3 |
| Type of Compound | Flavonoids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| Chemical Name | (E)-1-(2,4-dihydroxyphenyl)-3-(3-hydroxy-4-methoxyphenyl)prop-2-en-1-one | ||
| SMILES | COC1=C(C=C(C=C1)C=CC(=O)C2=C(C=C(C=C2)O)O)O | ||
| Standard InChIKey | WGVFVBIJKULVHA-QHHAFSJGSA-N | ||
| Standard InChI | InChI=1S/C16H14O5/c1-21-16-7-3-10(8-15(16)20)2-6-13(18)12-5-4-11(17)9-14(12)19/h2-9,17,19-20H,1H3/b6-2+ | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | 1. 4-O-Methylbutein (2',3,4'-trihydroxy-4-methoxychalcone) is a potent heme oxygenase-1 (HO-1) inducer, the upregulation of HO-1 has proven to be a useful tool for fighting inflammation,suggests that 4-O-methylbutein may have anti-inflammation activity. |
| Targets | HO-1 |
4-O-Methylbutein Dilution Calculator
4-O-Methylbutein Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 3.4928 mL | 17.4642 mL | 34.9284 mL | 69.8568 mL | 87.321 mL |
| 5 mM | 0.6986 mL | 3.4928 mL | 6.9857 mL | 13.9714 mL | 17.4642 mL |
| 10 mM | 0.3493 mL | 1.7464 mL | 3.4928 mL | 6.9857 mL | 8.7321 mL |
| 50 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.3971 mL | 1.7464 mL |
| 100 mM | 0.0349 mL | 0.1746 mL | 0.3493 mL | 0.6986 mL | 0.8732 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
- Heliangin
Catalog No.:BCN6487
CAS No.:13323-48-3
- AM 92016 hydrochloride
Catalog No.:BCC6825
CAS No.:133229-11-5
- PI3k-delta inhibitor 1
Catalog No.:BCC1861
CAS No.:1332075-63-4
- Fmoc-Thr(Trt)-OH
Catalog No.:BCC3554
CAS No.:133180-01-5
- Fmoc-Cit-OH
Catalog No.:BCC3180
CAS No.:133174-15-9
- Oxypeucedan hydrate
Catalog No.:BCN8372
CAS No.:133164-11-1
- GR 73632
Catalog No.:BCC5801
CAS No.:133156-06-6
- Epimedin B1
Catalog No.:BCN8199
CAS No.:133137-58-3
- Flutamide
Catalog No.:BCC4364
CAS No.:13311-84-7
- Darifenacin HBr
Catalog No.:BCC4567
CAS No.:133099-07-7
- Darifenacin
Catalog No.:BCC1516
CAS No.:133099-04-4
- 3PO
Catalog No.:BCC5616
CAS No.:13309-08-5
- GSK2194069
Catalog No.:BCC8053
CAS No.:1332331-08-4
- KPT-185
Catalog No.:BCC4444
CAS No.:1333151-73-7
- TRPC6 inhibitor
Catalog No.:BCC4199
CAS No.:1333207-63-8
- 10-Deacetylyunnanxane
Catalog No.:BCN7338
CAS No.:1333323-17-3
- Imbricataflavone A
Catalog No.:BCN8025
CAS No.:133336-96-6
- CHR-6494
Catalog No.:BCC1479
CAS No.:1333377-65-3
- Lactacystin
Catalog No.:BCN1841
CAS No.:133343-34-7
- Lydicamycin
Catalog No.:BCN1843
CAS No.:133352-27-9
- Daturataturin A
Catalog No.:BCN6179
CAS No.:133360-51-7
- DV 7028 hydrochloride
Catalog No.:BCC6202
CAS No.:133364-62-2
- Fmoc-His(MMt)-OH
Catalog No.:BCC3503
CAS No.:133367-33-6
- 4-Hydroxy-11,12,13-trinor-5-eudesmen-7-one
Catalog No.:BCN6627
CAS No.:133369-42-3
Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay.[Pubmed:25462643]
Free Radic Biol Med. 2015 Jan;78:135-46.
The upregulation of heme oxygenase-1 (HO-1) has proven to be a useful tool for fighting inflammation. In order to identify new HO-1 inducers, an efficient screening method was developed which can provide new lead structures for drug research. We designed a simple ELISA-based HO-1 enzyme activity assay, which allows for the screening of 12 compounds in parallel in the setting of a 96-well plate. The well-established murine macrophage cell line RAW264.7 is used and only about 26microg of protein from whole cell lysates is needed for the analysis of HO-1 activity. The quantification of HO-1 activity is based on an indirect ELISA using the specific anti-bilirubin antibody 24G7 to quantify directly bilirubin in the whole cell lysate, applying a horseradish peroxidase-tagged antibody together with ortho-phenylenediamine and H2O2 for detection. The bilirubin is produced on the action of HO enzymes by converting their substrate heme to biliverdin and additional recombinant biliverdin reductase together with NADPH at pH 7.4 in buffer. This sensitive assay allows for the detection of 0.57-82pmol bilirubin per sample in whole cell lysates. Twenty-three small molecules, mainly natural products with an alpha,beta-unsaturated carbonyl unit such as polyphenols, including flavonoids and chalcones, terpenes, an isothiocyanate, and the drug oltipraz were tested at typically 6 or 24h incubation with RAW264.7 cells. The activity of known HO-1 inducers was confirmed, while the chalcones cardamonin, flavokawain A, calythropsin, 2',3,4'-trihydroxy-4-methoxychalcone (THMC), and 2',4'-dihydroxy-3,4-dimethoxychalcone (DHDMC) were identified as new potent HO-1 inducers. The highest inductive power after 6h incubation was found at 10microM for DHDMC (6.1-fold), carnosol (3.9-fold), butein (3.1-fold), THMC (2.9-fold), and zerumbone (2.5-fold). Moreover, the time dependence of HO-1 protein production for DHDMC was compared to its enzyme activity, which was further evaluated in the presence of lipopolysaccharide and the specific HO-1 inhibitor tin protoporphyrin IX. Taken together, we developed a convenient and highly sensitive ELISA-based HO-1 enzyme activity assay, allowing the identification and characterization of molecules potentially useful for the treatment of inflammatory and autoimmune diseases.
