Angelicide

CAS# 92935-94-9

Angelicide

Catalog No. BCN8662----Order now to get a substantial discount!

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Quality Control of Angelicide

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Chemical structure

Angelicide

3D structure

Chemical Properties of Angelicide

Cas No. 92935-94-9 SDF Download SDF
PubChem ID 5316848 Appearance Powder
Formula C24H28O4 M.Wt 380.48
Type of Compound Miscellaneous Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (3'Z)-3'-butylidene-6'-propylspiro[4,5-dihydro-2-benzofuran-3,7'-5,5a,6,7a-tetrahydro-4H-cyclobuta[g][2]benzofuran]-1,1'-dione
SMILES CCCC=C1C2=C(C3C(CC2)C(C34C5=C(C=CCC5)C(=O)O4)CCC)C(=O)O1
Standard InChIKey TYSOMZQRYGBSKN-ODLFYWEKSA-N
Standard InChI InChI=1S/C24H28O4/c1-3-5-11-19-16-13-12-14-17(8-4-2)24(21(14)20(16)23(26)27-19)18-10-7-6-9-15(18)22(25)28-24/h6,9,11,14,17,21H,3-5,7-8,10,12-13H2,1-2H3/b19-11-
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Angelicide

The roots of Angelica sinensis

Biological Activity of Angelicide

In vitro

Secondary Metabolites Production in Angelica gigas Cell Culture by Two-Stage Process.[Reference: WebLink]

한국생물공학회 학술대회, 2008, 107(3):169-170.

Angelica gigas is a common traditional medicinal plant that has been used for more than several thousand years in Korea. The commonly used Angelica roots showed pharmacological properties with their chemical compositions. The main chemical constituents of Angelica are decursin, decursinol angelate, Angelicide, brefeldin A, succinic acid and adenine(2). Main constituents associated with the pharmacological activities of Angelica roots had anticancer activity(1), inhibitor of platelet aggregation, antiasthmatic and spasmolytic activities(2). The economic feasibility of production by plant cell culture is primarily determined by productivity. It has been argued that optimization of biomass growth and product formation must be evaluated as to the possibility of implementation in a large-scale process(3). One of solutions was a two-stage process.
METHODS AND RESULTS:
We have changed hormone condition of Angelica gigas cell culture for two-stage culture. After analyzing intracellular compounds of our Angelica gigas cell line, we have validated to increase several secondary metabolites after changing culture medium.
CONCLUSIONS:
In this study, we suggest that two-stage process of plant cell culture is a good system for secondary metabolites production.

Protocol of Angelicide

Structure Identification
Journal of Chromatography A, 1998, 810(1–2):71-79.

Liquid chromatographic-electrospray mass spectrometric study of Angelica sinensis and chemical changes of Z-ligustilide[Reference: WebLink]

High-performance liquid chromatography–electrospray ionization–mass spectrometry has been applied to analyze the chemical constituents of Danggui (the rhizome of Angelica sinensis) and to study chemical changes of Z-ligustilide.
METHODS AND RESULTS:
Twelve phthalides were unambiguously identified as senkyunolide I (3), senkyunolide H (4), sedanenolide (8), butylphthalide (9), E-ligustilide (13), Z-ligustilide (14), Z-butylidenephthalide (15), Z,Z′-6.8′,7.3′-diligustilide (16), Angelicide (17), levistolide A (18), Z-ligustilide dimer E-232 (19) and Z,Z′-3.3′,8.8′-diligustilide (20) in Danggui extract. The existence of 12 other phthalides (2, 5–7, 11, 12, 22–27), ferulic acid (1) and coniferyl ferulate (10) in Danggui extract has also been demonstrated. Phthalides 3, 4, 16–18 and 20 were determined to be the products from chemical change of Z-ligustilide.
CONCLUSIONS:
This is the first report of the existence of 16 compounds (2–8, 10–12, 20, 22–25 and 27) in Danggui extract.

Angelicide Dilution Calculator

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Angelicide Molarity Calculator

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Preparing Stock Solutions of Angelicide

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.6283 mL 13.1413 mL 26.2826 mL 52.5652 mL 65.7065 mL
5 mM 0.5257 mL 2.6283 mL 5.2565 mL 10.513 mL 13.1413 mL
10 mM 0.2628 mL 1.3141 mL 2.6283 mL 5.2565 mL 6.5706 mL
50 mM 0.0526 mL 0.2628 mL 0.5257 mL 1.0513 mL 1.3141 mL
100 mM 0.0263 mL 0.1314 mL 0.2628 mL 0.5257 mL 0.6571 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Angelicide

Liquid chromatographic-electrospray mass spectrometric study of Angelica sinensis and chemical changes of Z-ligustilide

Journal of Chromatography A, 1998, 810(1–2):71-79.

High-performance liquid chromatography–electrospray ionization–mass spectrometry has been applied to analyze the chemical constituents of Danggui (the rhizome of Angelica sinensis) and to study chemical changes of Z-ligustilide. Twelve phthalides were unambiguously identified as senkyunolide I (3), senkyunolide H (4), sedanenolide (8), butylphthalide (9), E-ligustilide (13), Z-ligustilide (14), Z-butylidenephthalide (15), Z,Z′-6.8′,7.3′-diligustilide (16), Angelicide (17), levistolide A (18), Z-ligustilide dimer E-232 (19) and Z,Z′-3.3′,8.8′-diligustilide (20) in Danggui extract. The existence of 12 other phthalides (2, 5–7, 11, 12, 22–27), ferulic acid (1) and coniferyl ferulate (10) in Danggui extract has also been demonstrated. Phthalides 3, 4, 16–18 and 20 were determined to be the products from chemical change of Z-ligustilide. This is the first report of the existence of 16 compounds (2–8, 10–12, 20, 22–25 and 27) in Danggui extract.

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