Astilbin

CAS# 29838-67-3

Astilbin

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Chemical structure

Astilbin

3D structure

Chemical Properties of Astilbin

Cas No. 29838-67-3 SDF Download SDF
PubChem ID 442437 Appearance White powder
Formula C21H22O11 M.Wt 450.4
Type of Compound Flavonoids Storage Desiccate at -20°C
Synonyms Taxifolin 3-rhamnoside
Solubility DMSO : ≥ 60 mg/mL (133.22 mM)
*"≥" means soluble, but saturation unknown.
Chemical Name (2S,3S)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy-2,3-dihydrochromen-4-one
SMILES CC1C(C(C(C(O1)OC2C(OC3=CC(=CC(=C3C2=O)O)O)C4=CC(=C(C=C4)O)O)O)O)O
Standard InChIKey ZROGCCBNZBKLEL-MFSALPCASA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Astilbin

1 Smilax sp.

Biological Activity of Astilbin

DescriptionAstilbin has insecticidal, antioxidant, antibacterial, and anti-inflammatory activities, it may act as an efficient therapeutic agent for arthritis like cyclosporine A but with less toxicity, its mechanism includes a selective suppression on lymphocyte functions via reducing MMP and NO production. Astilbin can exert an early renal protective role to diabetic nephropathy (DN), inhibit production of transforming growth factor-beta1 (TGF-beta1) and connective tissue growth factor (CTGF).Astilbin also alleviates contact hypersensitivity through a unique mechanism involving a negative cytokine regulation through stimulating IL-10, which is distinct from the immunosuppressant cyclosporin A.
TargetsTGF-β/Smad | IL Receptor | NF-kB | TNF-α | IFN-γ | NO | MMP(e.g.TIMP) | CTGF
In vitro

Isolation and in vitro antibacterial activity of astilbin, the bioactive flavanone from the leaves of Harungana madagascariensis Lam. ex Poir. (Hypericaceae).[Pubmed: 16483735 ]

J Ethnopharmacol. 2006 Jun 30;106(2):272-8.

Harungana madagascariensis is well known for its topical antibacterial properties used in the elaboration of a lot of skin hygiene products. The aim of this study was, on the one hand, to evaluate the in vitro antibacterial activities of aqueous, ethanolic and ethyl acetate crude extracts of Harungana madagascariensis leaves against bacterial strains representative of skin microflora and, on the other hand, to determine the chemical structure of the active compound. Only the ethyl acetate leaf extract presented important antibacterial activity.
METHODS AND RESULTS:
Its fractionation was carried out by column chromatography using silica gel 60 and it yielded 11 fractions. A bioautographic method, revealed in these fractions the presence of a flavanone as the active compound Astilbin or 3-O-alpha-L-rhamnoside-5,7,3',4'-tetrahydroxydihydroflavonol which was identified on the basis of its spectroscopic data. Concerning the antibacterial activity against the representative skin microflora of the armpit and feet, MIC and MBC ranged from 25 to 250 and 100 to 750 microg ml-1, respectively.
CONCLUSIONS:
The results showed that some bacteria considered to be responsible for bad odours at the armpit and feet levels, were destroyed at 200 microg ml-1 (MBC), a concentration sparing most of the useful saprophytic microflora. The minimal inhibitory quantity (MIQs) of Astilbin ranged from 50 to 100 microg.

Antioxidant activity of Rhizoma Smilacis Glabrae extracts and its key constituent-astilbin.[Reference: WebLink]

Food Chem.,2009,115(1):297-303.

Rhizoma Smilacis Glabrae is widely consumed by Chinese as functional food and in folk medicine for its medicinal properties. In this study, methanol and water extracts of Rhizoma Smilacis Glabrae were prepared. The water extract was further divided into polysaccharide and supernatant fractions.
METHODS AND RESULTS:
Constituents in different extracts were analysed by capillary electrophoresis, and levels of total phenolics were also determined using the Folin-Ciocalteu method. Astilbin, the main constituent in the herb, was isolated and purified. Different antioxidant tests were employed to evaluate the antioxidant activities of the extracts and the isolated Astilbin, and the results were compared with two commonly used synthetic antioxidants-butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Methanol, water extract and supernatant fraction showed concentration dependent antioxidant activity while polysaccharide didn’t show any antioxidant activity.
CONCLUSIONS:
Purified Astilbin showed the strongest antioxidant activity in comparison to any other extracts.

Biological activity of astilbin from Dimorphandra mollis against Anticarsia gemmatalis and Spodoptera frugiperda.[Pubmed: 11997979 ]

Pest Manag Sci. 2002 May;58(5):503-7.


METHODS AND RESULTS:
Astilbin was isolated in high yield from Dimorphandra mollis, and its insecticidal and growth inhibiting activity by stomach ingestion were evaluated against Anticarsia gemmatalis and Spodoptera frugiperda. The insecticidal activity of Astilbin, the weight reduction of the larval phase and the prolongation of the larval and pupal phases were verified for both species.
CONCLUSIONS:
Astilbin was identified on the base of its NMR, MS and physical data.

In vivo

Astilbin protects diabetic rat heart against ischemia-reperfusion injury via blockade of HMGB1-dependent NF-κB signaling pathway.[Pubmed: 24211745]

Food Chem Toxicol. 2014 Jan;63:104-10.

Astilbin, a flavonoid compound was isolated from the rhizome of Smilax china L.
METHODS AND RESULTS:
In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effect of Astilbin on diabetic rats in vivo and elucidated the potential mechanism in vitro. The results showed that Astilbin significantly attenuated hypoxia-induced cell injury in a concentration-dependent manner. Treatment of H9c2 cells with Astilbin at 15 μM blocked nuclear factor kappaB (NF-κB) phosphorylation by blocking High-mobility group box protein 1 (HMGB1) expression. Treatment of diabetic rats with Astilbin by intravenous injection (i.v.) at a single dose of 50 mg/kg protected the rats from myocardial I/R injury as indicated by decreasing infarct volume, improving hemodynamics and reducing myocardial damage, and also lowered serum levels of pro-inflammatory factors, reduced HMGB1 and phosphorylated NF-κB expression in ischemic myocardial tissue from diabetic rats. Additionally, treatment of diabetic rats with Astilbin at dose of 50 mg/kg by i.v. for continuous 14 days attenuated cardiac remodeling in the model myocardial I/R injury.
CONCLUSIONS:
These protective effects suggested that Astilbin might be due to block of the myocardial inflammatory cascade via the HMGB1-dependent NF-κB signaling pathway.

Astilbin prevents concanavalin A-induced liver injury by reducing TNF-alpha production and T lymphocytes adhesion.[Pubmed: 15104095]

J Pharm Pharmacol. 2004 Apr;56(4):495-502.

The aim of this study was to evaluate the effect of Astilbin on concanavalin A (Con A)-induced hepatitis, a T cell-dependent model of liver injury.
METHODS AND RESULTS:
Con A administration resulted in a severe liver injury in mice, with a strong increment in spleen cell adhesion and liver infiltration of T cells, as well as in tumour necrosis factor (TNF)-alpha production. Against this liver injury, Astilbin significantly inhibited the elevation in transaminase activity, reduced the TNF-alpha production, and improved the histological changes, including inflammatory infiltration, hepatocyte necrosis and degeneration and Kupffer cell hyperplasia. In addition, Astilbin inhibited the adhesion of spleen cells and purified T lymphocytes isolated from the liver-injured mice to fibronectin, laminin and type IV collagen.Moreover, the adhesion of human Jurkat T cells to endothelial cell line ECV-304 was also inhibited by Astilbin.
CONCLUSIONS:
These results suggest that the improvement of the T cell-mediated liver injury by Astilbinmay be related to the reduction in TNF-alpha production and in T cell adhesion to extracellular matrices and endothelial cells.

Protocol of Astilbin

Kinase Assay

Astilbin inhibits contact hypersensitivity through negative cytokine regulation distinct from cyclosporin A.[Pubmed: 16337470]

Effect of astilbin on experimental diabetic nephropathy in vivo and in vitro.[Pubmed: 19644810 ]

Planta Med. 2009 Nov;75(14):1470-5.

Astilbin, a flavonoid compound, was isolated from the rhizome of Smilax glabra Roxb. This study was conducted to investigate the efficacy of Astilbin on experimental diabetic nephropathy (DN) in vivo and in vitro and its possible mechanisms.
METHODS AND RESULTS:
Astilbin was added in high glucose stimulated HK-2 cells, streptozotocin-induced experimental DN, randomized to receive intragastric ( I. G.) Astilbin to observe its anti-renal lesion effect. Results showed that Astilbin inhibited high glucose stimulated HK-2 cell production of transforming growth factor-beta1 (TGF-beta1) and connective tissue growth factor (CTGF) in vitro, especially CTGF; analogic results was also found in vivo. I. G. of Astilbin 2.5 mg/kg or 5 mg/kg significantly ameliorated renal function, reduced kidney index, while it increased body weight and survival time in animals. In addition there was no significant difference in blood glucose level between the STZ-treated group and the Astilbin groups. Furthermore, Astilbin ameliorated the pathological progress of renal morphology. Astilbin can exert an early renal protective role to DN, inhibit production of TGF-beta1 and especially of CTGF.
CONCLUSIONS:
We suggest that Astilbin inhibition of CTGF may be a potential target in DN therapy. This work provides the first evidence for Astilbin as a new candidate of DN therapeutic medicine.

J Allergy Clin Immunol. 2005 Dec;116(6):1350-6.

IL-10 is known as a negative regulator for inflammatory diseases, including contact dermatitis. However, only a few drug candidates are reported to induce endogenous IL-10. We sought to elucidate a new mechanism underlying the immunosuppressive properties of Astilbin through negative cytokine regulation in comparison with the effective pattern with cyclosporin A.
METHODS AND RESULTS:
Contact hypersensitivity was induced in mice with picryl chloride. Lymph node cells were isolated for adoptive transfer and cytokine assays. Astilbin significantly inhibited contact hypersensitivity when given in the elicitation phase but not in the sensitization phase, whereas cyclosporin A inhibited both phases. Lymph node cells from donor mice administered Astilbin failed to adoptively transfer the hypersensitivity. Astilbin in vivo remarkably induced IL-10 expression in lymph node cells at an earlier time and decreased TNF-alpha and IFN-gamma expression at a later time. Furthermore, the in vivo neutralization of IL-10 significantly impaired the effect of Astilbin on contact hypersensitivity. In the isolated lymphocytes sensitized with picryl chloride in vivo and challenged with trinitrobenzene-sulfonic acid in vitro, Astilbin did not affect the cell proliferation but modulated the above cytokine profiles as its in vivo effect in a concentration-dependent manner and furthermore significantly enhanced the expressions of suppressor of cytokine signaling 1 and 3. On the other hand, cyclosporin A strongly inhibited proinflammatory cytokine production but influenced neither IL-10 nor downstream suppressor of cytokine signaling 1 and 3 expression.
CONCLUSIONS:
Astilbin alleviates contact hypersensitivity through a unique mechanism involving a negative cytokine regulation through stimulating IL-10, which is distinct from the immunosuppressant cyclosporin A.

Cell Research

Astilbin selectively facilitates the apoptosis of interleukin-2-dependent phytohemagglutinin-activated Jurkat cells.[Pubmed: 11516264 ]

Pharmacol Res. 2001 Aug;44(2):135-9.

The present study examined the relationship between the activation of T cells and the apoptosis-facilitating effect of Astilbin on them.
METHODS AND RESULTS:
By the stimulation of PHA, a remarkable IL-2 production was detected in the supernatant of Jurkat cells after 120 h among 72--144 h incubation. This kinetics was quite in accordance with that of Astilbin-induced apoptosis of Jurkat cells, where 1 h-exposure of the PHA-activated cells to Astilbin caused a significantly increased apoptosis in a dose-dependent manner. To the Jurkat cells that had been cultivated for 72--144 h without PHA, however, Astilbin did not show any facilitation of the cell apoptosis. Pre-treatment by cyclosporine A simultaneously with PHA dose-dependently lowered the IL-2 production and susceptibility of the cells to Astilbin, while the treatment after 120 h of PHA-activation did not. The exogenous IL-2 treatment after 72 h of PHA-activation significantly and dose-dependently raised the susceptibility of the Jurkat cells to Astilbin.
CONCLUSIONS:
These results indicated the dependency of the apoptosis-facilitating effect of Astilbin on appropriate status of activated T lymphocytes with a relation to IL-2 production. This characteristic of Astilbin may be of great significance for the treatment of a variety of immunologically related diseases.

Animal Research

Astilbin suppresses collagen-induced arthritis via the dysfunction of lymphocytes.[Pubmed: 14504671 ]

Induction of TGF-β and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis.[Pubmed: 24613838]

Biochem Biophys Res Commun. 2014 Apr 4;446(2):529-34.

Astilbin, a major bioactive compound from Rhizoma smilacis glabrae, has been reported to possess anti-inflammatory properties.
METHODS AND RESULTS:
Our study first evaluated Astilbin on dextran sulfate sodium (DSS)-induced acute colitis in mice. By intraperitoneal injection of Astilbin, the severity of colitis was attenuated, and the serum levels of IL-10 and TGF-β were increased. Using flow cytometry, a higher number of IL-10(+) dendritic cells (DCs) and TGF-β(+) DCs and a lower number of CD86(+) DCs, IL-12 p40(+) DCs, and IL-1β(+) DCs were detected in the spleen of mice with colitis after Astilbin treatment. The administration of Astilbin also resulted in the upregulation of CD103(+) expression in colonic DCs. In a coculture system, murine bone marrow-derived DCs pretreated with Astilbin resulted in an enhanced production of CD4(+)CD25(+)Foxp3(+) T cells.
CONCLUSIONS:
The results of this study show that Astilbin could be a candidate drug for inflammatory bowel disease by mediating the regulatory functions of DCs.

Inflamm Res. 2003 Aug;52(8):334-40.

To examine the therapeutic effects of Astilbin, a flavanoid isolated from Rhizoma Smilacis Glabrae, on arthritis and to compare it with cyclosporine A (CsA).
METHODS AND RESULTS:
Type II collagen-induced arthritis in mice and its in vitro assays for proliferation, matrix metalloproteinase (MMP) and NO production were performed. Astilbin dose-dependently inhibited the footpad swelling, arthritic incidence, and clinical scores without influencing the body weights, while CsA showed strong inhibition with a significant weight loss. Histological examination revealed marked inflammatory damage in arthritic mice including joint swelling, synovial hyperplasia, and cartilage destruction. Against these, an intact joint structure was maintained in Astilbin-treated or CsA-treated mice. In isolated spleen cells from arthritic mice, increased potentials in proliferation, NO production, and MMP-2 and 9 activities were suppressed dose-dependently by the oral administration of Astilbin. Additionally, Astilbin showed neither any cytotoxicity to nor influence on Con A-induced proliferation of spleen cells from naive mice, while CsA showed a dose-dependent cytotoxicity and inhibition of the proliferation.
CONCLUSIONS:
Astilbin may act as an efficient therapeutic agent for arthritis like CsA but with less toxicity. Its mechanism includes a selective suppression on lymphocyte functions via reducing MMP and NO production.

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Preparing Stock Solutions of Astilbin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.2202 mL 11.1012 mL 22.2025 mL 44.405 mL 55.5062 mL
5 mM 0.444 mL 2.2202 mL 4.4405 mL 8.881 mL 11.1012 mL
10 mM 0.222 mL 1.1101 mL 2.2202 mL 4.4405 mL 5.5506 mL
50 mM 0.0444 mL 0.222 mL 0.444 mL 0.8881 mL 1.1101 mL
100 mM 0.0222 mL 0.111 mL 0.222 mL 0.444 mL 0.5551 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Astilbin

Astilbin suppresses collagen-induced arthritis via the dysfunction of lymphocytes.[Pubmed:14504671]

Inflamm Res. 2003 Aug;52(8):334-40.

OBJECTIVE AND DESIGN: To examine the therapeutic effects of Astilbin, a flavanoid isolated from Rhizoma Smilacis Glabrae, on arthritis and to compare it with cyclosporine A (CsA). MATERIALS AND METHODS: Type II collagen-induced arthritis in mice and its in vitro assays for proliferation, matrix metalloproteinase (MMP) and NO production were performed. RESULTS: Astilbin dose-dependently inhibited the footpad swelling, arthritic incidence, and clinical scores without influencing the body weights, while CsA showed strong inhibition with a significant weight loss. Histological examination revealed marked inflammatory damage in arthritic mice including joint swelling, synovial hyperplasia, and cartilage destruction. Against these, an intact joint structure was maintained in Astilbin-treated or CsA-treated mice. In isolated spleen cells from arthritic mice, increased potentials in proliferation, NO production, and MMP-2 and 9 activities were suppressed dose-dependently by the oral administration of Astilbin. Additionally, Astilbin showed neither any cytotoxicity to nor influence on Con A-induced proliferation of spleen cells from naive mice, while CsA showed a dose-dependent cytotoxicity and inhibition of the proliferation. CONCLUSIONS: Astilbin may act as an efficient therapeutic agent for arthritis like CsA but with less toxicity. Its mechanism includes a selective suppression on lymphocyte functions via reducing MMP and NO production.

Induction of TGF-beta and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis.[Pubmed:24613838]

Biochem Biophys Res Commun. 2014 Apr 4;446(2):529-34.

Astilbin, a major bioactive compound from Rhizoma smilacis glabrae, has been reported to possess anti-inflammatory properties. Our study first evaluated Astilbin on dextran sulfate sodium (DSS)-induced acute colitis in mice. By intraperitoneal injection of Astilbin, the severity of colitis was attenuated, and the serum levels of IL-10 and TGF-beta were increased. Using flow cytometry, a higher number of IL-10(+) dendritic cells (DCs) and TGF-beta(+) DCs and a lower number of CD86(+) DCs, IL-12 p40(+) DCs, and IL-1beta(+) DCs were detected in the spleen of mice with colitis after Astilbin treatment. The administration of Astilbin also resulted in the upregulation of CD103(+) expression in colonic DCs. In a coculture system, murine bone marrow-derived DCs pretreated with Astilbin resulted in an enhanced production of CD4(+)CD25(+)Foxp3(+) T cells. The results of this study show that Astilbin could be a candidate drug for inflammatory bowel disease by mediating the regulatory functions of DCs.

Astilbin inhibits contact hypersensitivity through negative cytokine regulation distinct from cyclosporin A.[Pubmed:16337470]

J Allergy Clin Immunol. 2005 Dec;116(6):1350-6.

BACKGROUND: IL-10 is known as a negative regulator for inflammatory diseases, including contact dermatitis. However, only a few drug candidates are reported to induce endogenous IL-10. OBJECTIVE: We sought to elucidate a new mechanism underlying the immunosuppressive properties of Astilbin through negative cytokine regulation in comparison with the effective pattern with cyclosporin A. METHODS: Contact hypersensitivity was induced in mice with picryl chloride. Lymph node cells were isolated for adoptive transfer and cytokine assays. RESULTS: Astilbin significantly inhibited contact hypersensitivity when given in the elicitation phase but not in the sensitization phase, whereas cyclosporin A inhibited both phases. Lymph node cells from donor mice administered Astilbin failed to adoptively transfer the hypersensitivity. Astilbin in vivo remarkably induced IL-10 expression in lymph node cells at an earlier time and decreased TNF-alpha and IFN-gamma expression at a later time. Furthermore, the in vivo neutralization of IL-10 significantly impaired the effect of Astilbin on contact hypersensitivity. In the isolated lymphocytes sensitized with picryl chloride in vivo and challenged with trinitrobenzene-sulfonic acid in vitro, Astilbin did not affect the cell proliferation but modulated the above cytokine profiles as its in vivo effect in a concentration-dependent manner and furthermore significantly enhanced the expressions of suppressor of cytokine signaling 1 and 3. On the other hand, cyclosporin A strongly inhibited proinflammatory cytokine production but influenced neither IL-10 nor downstream suppressor of cytokine signaling 1 and 3 expression. CONCLUSION: Astilbin alleviates contact hypersensitivity through a unique mechanism involving a negative cytokine regulation through stimulating IL-10, which is distinct from the immunosuppressant cyclosporin A.

Isolation and in vitro antibacterial activity of astilbin, the bioactive flavanone from the leaves of Harungana madagascariensis Lam. ex Poir. (Hypericaceae).[Pubmed:16483735]

J Ethnopharmacol. 2006 Jun 30;106(2):272-8.

Harungana madagascariensis is well known for its topical antibacterial properties used in the elaboration of a lot of skin hygiene products. The aim of this study was, on the one hand, to evaluate the in vitro antibacterial activities of aqueous, ethanolic and ethyl acetate crude extracts of Harungana madagascariensis leaves against bacterial strains representative of skin microflora and, on the other hand, to determine the chemical structure of the active compound. Only the ethyl acetate leaf extract presented important antibacterial activity. Its fractionation was carried out by column chromatography using silica gel 60 and it yielded 11 fractions. A bioautographic method, revealed in these fractions the presence of a flavanone as the active compound Astilbin or 3-O-alpha-L-rhamnoside-5,7,3',4'-tetrahydroxydihydroflavonol which was identified on the basis of its spectroscopic data. Concerning the antibacterial activity against the representative skin microflora of the armpit and feet, MIC and MBC ranged from 25 to 250 and 100 to 750 microg ml-1, respectively. The results showed that some bacteria considered to be responsible for bad odours at the armpit and feet levels, were destroyed at 200 microg ml-1 (MBC), a concentration sparing most of the useful saprophytic microflora. The minimal inhibitory quantity (MIQs) of Astilbin ranged from 50 to 100 microg.

Astilbin prevents concanavalin A-induced liver injury by reducing TNF-alpha production and T lymphocytes adhesion.[Pubmed:15104095]

J Pharm Pharmacol. 2004 Apr;56(4):495-502.

The aim of this study was to evaluate the effect of Astilbin on concanavalin A (Con A)-induced hepatitis, a T cell-dependent model of liver injury. Con A administration resulted in a severe liver injury in mice, with a strong increment in spleen cell adhesion and liver infiltration of T cells, as well as in tumour necrosis factor (TNF)-alpha production. Against this liver injury, Astilbin significantly inhibited the elevation in transaminase activity, reduced the TNF-alpha production, and improved the histological changes, including inflammatory infiltration, hepatocyte necrosis and degeneration and Kupffer cell hyperplasia. In addition, Astilbin inhibited the adhesion of spleen cells and purified T lymphocytes isolated from the liver-injured mice to fibronectin, laminin and type IV collagen.Moreover, the adhesion of human Jurkat T cells to endothelial cell line ECV-304 was also inhibited by Astilbin. These results suggest that the improvement of the T cell-mediated liver injury by Astilbinmay be related to the reduction in TNF-alpha production and in T cell adhesion to extracellular matrices and endothelial cells.

Astilbin protects diabetic rat heart against ischemia-reperfusion injury via blockade of HMGB1-dependent NF-kappaB signaling pathway.[Pubmed:24211745]

Food Chem Toxicol. 2014 Jan;63:104-10.

Astilbin, a flavonoid compound was isolated from the rhizome of Smilax china L. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effect of Astilbin on diabetic rats in vivo and elucidated the potential mechanism in vitro. The results showed that Astilbin significantly attenuated hypoxia-induced cell injury in a concentration-dependent manner. Treatment of H9c2 cells with Astilbin at 15 muM blocked nuclear factor kappaB (NF-kappaB) phosphorylation by blocking High-mobility group box protein 1 (HMGB1) expression. Treatment of diabetic rats with Astilbin by intravenous injection (i.v.) at a single dose of 50 mg/kg protected the rats from myocardial I/R injury as indicated by decreasing infarct volume, improving hemodynamics and reducing myocardial damage, and also lowered serum levels of pro-inflammatory factors, reduced HMGB1 and phosphorylated NF-kappaB expression in ischemic myocardial tissue from diabetic rats. Additionally, treatment of diabetic rats with Astilbin at dose of 50 mg/kg by i.v. for continuous 14 days attenuated cardiac remodeling in the model myocardial I/R injury. These protective effects suggested that Astilbin might be due to block of the myocardial inflammatory cascade via the HMGB1-dependent NF-kappaB signaling pathway.

Effect of astilbin on experimental diabetic nephropathy in vivo and in vitro.[Pubmed:19644810]

Planta Med. 2009 Nov;75(14):1470-5.

Astilbin, a flavonoid compound, was isolated from the rhizome of Smilax glabra Roxb. This study was conducted to investigate the efficacy of Astilbin on experimental diabetic nephropathy (DN) in vivo and in vitro and its possible mechanisms. Astilbin was added in high glucose stimulated HK-2 cells, streptozotocin-induced experimental DN, randomized to receive intragastric ( I. G.) Astilbin to observe its anti-renal lesion effect. Results showed that Astilbin inhibited high glucose stimulated HK-2 cell production of transforming growth factor-beta1 (TGF-beta1) and connective tissue growth factor (CTGF) in vitro, especially CTGF; analogic results was also found in vivo. I. G. of Astilbin 2.5 mg/kg or 5 mg/kg significantly ameliorated renal function, reduced kidney index, while it increased body weight and survival time in animals. In addition there was no significant difference in blood glucose level between the STZ-treated group and the Astilbin groups. Furthermore, Astilbin ameliorated the pathological progress of renal morphology. Astilbin can exert an early renal protective role to DN, inhibit production of TGF-beta1 and especially of CTGF. We suggest that Astilbin inhibition of CTGF may be a potential target in DN therapy. This work provides the first evidence for Astilbin as a new candidate of DN therapeutic medicine.

Astilbin selectively facilitates the apoptosis of interleukin-2-dependent phytohemagglutinin-activated Jurkat cells.[Pubmed:11516264]

Pharmacol Res. 2001 Aug;44(2):135-9.

The present study examined the relationship between the activation of T cells and the apoptosis-facilitating effect of Astilbin on them. By the stimulation of PHA, a remarkable IL-2 production was detected in the supernatant of Jurkat cells after 120 h among 72--144 h incubation. This kinetics was quite in accordance with that of Astilbin-induced apoptosis of Jurkat cells, where 1 h-exposure of the PHA-activated cells to Astilbin caused a significantly increased apoptosis in a dose-dependent manner. To the Jurkat cells that had been cultivated for 72--144 h without PHA, however, Astilbin did not show any facilitation of the cell apoptosis. Pre-treatment by cyclosporine A simultaneously with PHA dose-dependently lowered the IL-2 production and susceptibility of the cells to Astilbin, while the treatment after 120 h of PHA-activation did not. The exogenous IL-2 treatment after 72 h of PHA-activation significantly and dose-dependently raised the susceptibility of the Jurkat cells to Astilbin. These results indicated the dependency of the apoptosis-facilitating effect of Astilbin on appropriate status of activated T lymphocytes with a relation to IL-2 production. This characteristic of Astilbin may be of great significance for the treatment of a variety of immunologically related diseases.

Biological activity of astilbin from Dimorphandra mollis against Anticarsia gemmatalis and Spodoptera frugiperda.[Pubmed:11997979]

Pest Manag Sci. 2002 May;58(5):503-7.

Astilbin was isolated in high yield from Dimorphandra mollis, and its insecticidal and growth inhibiting activity by stomach ingestion were evaluated against Anticarsia gemmatalis and Spodoptera frugiperda. The insecticidal activity of Astilbin, the weight reduction of the larval phase and the prolongation of the larval and pupal phases were verified for both species. Astilbin was identified on the base of its NMR, MS and physical data.

Description

Astilbin, a flavonoid compound, is isolated from the rhizome of Smilax glabra. Astilbin enhances NRF2 activation. Astilbin also suppresses TNF-α expression and NF-κB activation.

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