Blasticidin S HClantibiotic, inhibits protein synthesis CAS# 3513-03-9 |
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Quality Control & MSDS
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| Cas No. | 3513-03-9 | SDF | Download SDF |
| PubChem ID | 75412545 | Appearance | Powder |
| Formula | C17H27ClN8O5 | M.Wt | 458.9 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Solubility | H2O : 20 mg/mL (43.58 mM; Need ultrasonic) | ||
| Chemical Name | (2S,3S,6R)-3-[[(3S)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride | ||
| SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl | ||
| Standard InChIKey | YQXYQOXRCNEATG-NMQKUDMSSA-N | ||
| Standard InChI | InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H/t9-,10-,13+,14-;/m0./s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | Antibiotic. Selection reagent for bis, bsr and BSD transformed cells. |
Blasticidin S HCl Dilution Calculator
Blasticidin S HCl Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.1791 mL | 10.8956 mL | 21.7912 mL | 43.5825 mL | 54.4781 mL |
| 5 mM | 0.4358 mL | 2.1791 mL | 4.3582 mL | 8.7165 mL | 10.8956 mL |
| 10 mM | 0.2179 mL | 1.0896 mL | 2.1791 mL | 4.3582 mL | 5.4478 mL |
| 50 mM | 0.0436 mL | 0.2179 mL | 0.4358 mL | 0.8716 mL | 1.0896 mL |
| 100 mM | 0.0218 mL | 0.109 mL | 0.2179 mL | 0.4358 mL | 0.5448 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Blasticidin S HCl is an antibiotic inhibiting protein synthesis [1].
Blasticidin S is isolated from Streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells.
Blasticidin S HCl is an antibiotic inhibiting protein synthesis. In mouse leukemia L5178Y cells, blasticidin S inhibited protein synthesis and caused the degradation of presumptive mRNA [1]. Also, blasticidin S inhibited ribonuclease P activity from Dictyostelium discoideum and inhibited tRNA maturation in a dose dependent way [2]. In S. cerevisiae, blasticidin S inhibited protein synthesis induced by galactose. In Aspergillus parasiticus, blasticidin S inhibited aflatoxin production without inhibition of fungal growth. In Aspergillus flavus, blasticidin S significantly reduced aflatoxin biosynthetic enzymes [3]. Blasticidin S efficiently bound Cu2+ ions and protected DNA from metal-induced damage [4]. Blasticidin S enhanced the binding of tRNA to the P site of the large ribosomal subunit and slowed spontaneous intersubunit rotation. Blasticidin S inhibited peptide bond formation and peptidyl-tRNA hydrolysis [5].
References:
[1]. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochim Biophys Acta, 1978, 521(1): 295-307.
[2]. Stathopoulos C, Tsagla A, Tekos A, et al. Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Effect of antibiotics on RNase P. Mol Biol Rep, 2000, 27(2): 107-111.
[3]. Yoshinari T, Noda Y, Yoda K, et al. Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors. J Antibiot (Tokyo), 2010, 63(6): 309-314.
[4]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem, 2013, 127: 73-78.
[5]. Svidritskiy E, Ling C, Ermolenko DN, et al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A, 2013, 110(30): 12283-12288.
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Effects of miR-19b overexpression on proliferation, differentiation, apoptosis and Wnt/beta-catenin signaling pathway in P19 cell model of cardiac differentiation in vitro.[Pubmed:23443808]
Cell Biochem Biophys. 2013 Jul;66(3):709-22.
MicroRNA (miR)-19b is part of the miR-17-92 cluster associated with cardiac development. Here, we investigated the effects of overexpressing miR-19b on proliferation, differentiation, apoptosis, and regulation of the Wnt/beta-catenin signaling pathway in the multipotent murine P19 cell line that can be induced to undergo cardiogenesis. P19 cells were transfected with the miR-19b plasmid or empty vector, and miR-19b overexpression was verified by Quantitative Real-Time PCR (qPCR). The miR-19b or vector control stable cell lines were selected using Blasticidin S HCl, and their proliferation, cell cycle, and apoptosis levels were analyzed using the Cell Counting Kit-8 and flow cytometry. P19 cell differentiation markers, apoptosis-related genes (bax, bcl-2), and Wnt/beta-catenin signaling pathway-related genes were detected by qPCR, the corresponding proteins by Western blot. Expression of the Wnt pathway and differentiation marker proteins was also verified by immunofluorescence. Morphological changes associated with apoptosis were observed by electron microscopy and Hoechst staining. On the basis of these results, we demonstrated that miR-19b overexpression promoted proliferation and differentiation but inhibited apoptosis in P19 cells; Wnt and beta-catenin expressions were decreased, while that of GSK3beta was increased with miR-19b overexpression. Overexpression of miR-19b inhibited activation of the Wnt/beta-catenin signaling pathway in P19 cells, which may regulate cardiomyocyte differentiation. Our findings may bring new insights into the mechanisms underlying cardiac diseases and suggest that miR-19b is a potential new therapeutic target for cardiovascular diseases.
