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Epistephamiersine

CAS# 52389-15-8

Epistephamiersine

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Quality Control of Epistephamiersine

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Chemical structure

Epistephamiersine

3D structure

Chemical Properties of Epistephamiersine

Cas No. 52389-15-8 SDF Download SDF
PubChem ID 91895297 Appearance Powder
Formula C21H27NO6 M.Wt 389.5
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (1S,8S,10S,11R,12S)-3,4,11,12-tetramethoxy-17-methyl-18-oxa-17-azapentacyclo[8.4.3.18,11.01,10.02,7]octadeca-2(7),3,5-trien-13-one
SMILES CN1CCC23C14CC(C5=C2C(=C(C=C5)OC)OC)OC4(C(C(=O)C3)OC)OC
Standard InChIKey UTTZNWQGZHNUIG-JMMIECQRSA-N
Standard InChI InChI=1S/C21H27NO6/c1-22-9-8-19-10-13(23)18(26-4)21(27-5)20(19,22)11-15(28-21)12-6-7-14(24-2)17(25-3)16(12)19/h6-7,15,18H,8-11H2,1-5H3/t15-,18-,19-,20-,21-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Epistephamiersine

The roots of Stephania japonica

Biological Activity of Epistephamiersine

DescriptionStandard reference

Protocol of Epistephamiersine

Structure Identification
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(15):1973-7.

Hasubanan type alkaloids in Stephania hernandifolia.[Pubmed: 20931849]

To study the hasubanan type alkaloids in Stephania hernandifolia.
METHODS AND RESULTS:
The dried herbs of S. hernandifolia. were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatograghic methods with on silica gel, Rp-18, MCI CHP 20P, Sephadex LH-20 were applied for the isolation and purification of the crude alkaloid fraction. The structures were elucidated by their physicochemical properties and spectral data. Nine hasubanan type alkaloids were obtained and identified as aknadinine(1), longanone(2), stephasunoline (3), N-methylstephuline(4), Epistephamiersine(5), prostephabyssine(6), aknadilactam(7), dihydroEpistephamiersine(8), hasubanonine(9).
CONCLUSIONS:
Compounds 2-8 were isolated from this plant for the first time.

Epistephamiersine Dilution Calculator

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Epistephamiersine Molarity Calculator

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Preparing Stock Solutions of Epistephamiersine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.5674 mL 12.837 mL 25.6739 mL 51.3479 mL 64.1849 mL
5 mM 0.5135 mL 2.5674 mL 5.1348 mL 10.2696 mL 12.837 mL
10 mM 0.2567 mL 1.2837 mL 2.5674 mL 5.1348 mL 6.4185 mL
50 mM 0.0513 mL 0.2567 mL 0.5135 mL 1.027 mL 1.2837 mL
100 mM 0.0257 mL 0.1284 mL 0.2567 mL 0.5135 mL 0.6418 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Epistephamiersine

[Hasubanan type alkaloids in Stephania hernandifolia].[Pubmed:20931849]

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(15):1973-7.

OBJECTIVE: To study the hasubanan type alkaloids in Stephania hernandifolia. METHOD: The dried herbs of S. hernandifolia. were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatograghic methods with on silica gel, Rp-18, MCI CHP 20P, Sephadex LH-20 were applied for the isolation and purification of the crude alkaloid fraction. The structures were elucidated by their physicochemical properties and spectral data. RESULT: Nine hasubanan type alkaloids were obtained and identified as aknadinine(1), longanone(2), stephasunoline (3), N-methylstephuline(4), Epistephamiersine(5), prostephabyssine(6), aknadilactam(7), dihydroEpistephamiersine(8), hasubanonine(9). CONCLUSION: Compounds 2-8 were isolated from this plant for the first time.

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