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Glycerol

CAS# 56-81-5

Glycerol

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Quality Control of Glycerol

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Chemical structure

Glycerol

3D structure

Chemical Properties of Glycerol

Cas No. 56-81-5 SDF Download SDF
PubChem ID 753 Appearance Powder
Formula C3H8O3 M.Wt 92
Type of Compound N/A Storage Desiccate at -20°C
Synonyms Glycerin
Solubility DMSO : ≥ 300 mg/mL (3257.68 mM)
*"≥" means soluble, but saturation unknown.
Chemical Name propane-1,2,3-triol
SMILES C(C(CO)O)O
Standard InChIKey PEDCQBHIVMGVHV-UHFFFAOYSA-N
Standard InChI InChI=1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Glycerol

DescriptionWidely used in sample buffer preparation.

Glycerol Dilution Calculator

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Glycerol Molarity Calculator

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Preparing Stock Solutions of Glycerol

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 10.8696 mL 54.3478 mL 108.6957 mL 217.3913 mL 271.7391 mL
5 mM 2.1739 mL 10.8696 mL 21.7391 mL 43.4783 mL 54.3478 mL
10 mM 1.087 mL 5.4348 mL 10.8696 mL 21.7391 mL 27.1739 mL
50 mM 0.2174 mL 1.087 mL 2.1739 mL 4.3478 mL 5.4348 mL
100 mM 0.1087 mL 0.5435 mL 1.087 mL 2.1739 mL 2.7174 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on Glycerol

Glycerol is a clear, colourless, viscous, sweet-tasting liquid. Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

In Vitro:Glycerol is often included in polyacrylamide gels to prevent dissociation of nucleosomes and other protein-DNA complexes during electrophoresis. With glycerol included, fractionation seems to be largely based on particle mass and charge. The concentration of glycerol during electrophoresis strongly affects the separation characteristics of polyacrylamide gels[1]. Glycerol is an inevitable by-product of oils/fats processing, regardless of the pathway. Fermentative metabolism of glycerol has been studied in great detail in several species of the Enterobacteriaceae family, such as Citrobacter freundii and Klebsiella pneumoniae. The use of anaerobic fermentation to convert abundant and low-priced glycerol streams generated in the production of biodiesel into higher value products represents a promising route to achieve economic viability in the biofuels industry[2].

In Vivo:Glycerol can induce acute renal failure in rat models. Acute renal failure induced by glycerol or uranyl nitrate reduces the hepato-biliary transport of some drugs, modulates the distribution of drugs into the central nervous system and affects the activity of various hepatic microsomal enzymes [3].

References:
[1]. Pennings S, et al. Effect of glycerol on the separation of nucleosomes and bent DNA in low ionic strengthpolyacrylamide gel electrophoresis. Nucleic Acids Res. 1992 Dec 25;20(24):6667-72. [2]. Yazdani SS, et al. Anaerobic fermentation of glycerol: a path to economic viability for the biofuelsindustry. Curr Opin Biotechnol. 2007 Jun;18(3):213-9. [3]. Huang ZH, et al. Expression and function of P-glycoprotein in rats with glycerol-induced acute renal failure. Eur J Pharmacol. 2000 Oct 20;406(3):453-60.

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References on Glycerol

How Glycerol and Water Contents Affect the Structural and Functional Properties of Starch-Based Edible Films.[Pubmed:30966447]

Polymers (Basel). 2018 Apr 8;10(4). pii: polym10040412.

As starch is an inexpensive, filmogenic, easily processable and a widely available material, it is a material that can be utilized in the creation of biodegradable films and containers, presenting as a viable alternative to polymers derived from petrol. Moreover, starch could also be used to create edible coatings for fresh foods in order to extend shelf life. As such, wheat starch films with two Glycerol contents were formulated to mimic the effects of compounds currently used to coat fruit. Their structural and functional properties were characterized. This study found that the transfer properties of starch films containing 33% of plasticizer was less effective than film comprised of 50% Glycerol. Water diffusivity, oxygen permeability, and water vapor permeability at two different humidity gradients, surface tension, works of surface adhesion and cohesion, and moisture sorption were tested. Glycerol content does not play a significant role on the color or mechanical properties. This work shows that Glycerol can strongly affect the functional properties of starch-based coatings and films.

Effect of Cholesterol-loaded Cyclodextrin on Membrane and Acrosome Status of Hariana Bull Sperm during Cryopreservation.[Pubmed:30963156]

Cryo Letters. 2018 Nov/Dec;39(6):386-390.

BACKGROUND: The membrane and acrosomal integrity of sperm play a vital role in fertilization process; however they are compromised upon cryopreservation. OBJECTIVE: To study the effect of cholesterol-loaded cyclodextrin (CLC) on membrane and acrosome status of Hariana bull sperm during cryopreservation. MATERIALS AND METHODS: Semen samples collected from Hariana bulls with mass motility >/= 3+ and individual progressive motility >/= 70% were utilized in the study. Each ejaculate was split into two parts, one part being evaluated freshly for various seminal attributes and the other part being diluted in Tris diluent (without egg yolk and Glycerol) to obtain a final concentration of 120x10(6) sperm/mL. The diluted semen was divided into four treatments: Group I, without CLC (control); Group II, with CLC at 0.5 mg per 120 million sperm; Group III, at 1.0 mg per 120 million sperm; Group IV, at 2.0 mg per 120 million sperm. All aliquots were incubated for 15 min at 37 degrees C and each sample was diluted with Egg yolk-Tris-Glycerol (EYTG) extender up to 80x10(6) sperm/mL. The diluted semen samples were packed in French mini straws (0.25 mL), sealed and equilibrated at 4 degrees C for 4 h followed by cryopreservation. The samples at pre-freeze and post-thaw stage were evaluated for membrane and acrosomal integrity, as well as primary, secondary and tertiary acrosomal damages. RESULTS: The membrane and acrosomal integrity was significantly higher in group II as compared to groups I, III, and IV, at pre-freeze and post-thaw stage (P<0.05). The primary and secondary acrosomal damage were significantly reduced in group II compared to other groups (P<0.05). No significant difference in tertiary acrosomal damage was found among different groups. CONCLUSION: CLC improves the membrane and acrosomal integrity, and reduces primary and secondary acrosomal damages during cryopreservation of Hariana bull sperm.

Description

Glycerol is a clear, colourless, viscous, sweet-tasting liquid. Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

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