Magnoloside B

[Chemical components of Magnoliae Officinalis Cortex of different origins and with different tree ages before and after being processed with ginger juice:a qualitative and quantitative analysis].[Pubmed:37282873]

Zhongguo Zhong Yao Za Zhi. 2023 May;48(9):2435-2454.

This study aimed to investigate the impact of ginger juice on chemical profile of Magnoliae Officinalis Cortex(MOC) when they were processed together. Ultra-high-performance liquid chromatography coupled to quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS) was used for qualitative analysis of the chemical component of MOC samples before and after being processed with ginger juice. UPLC was performed to observe the content variation of eight main components in processed MOC. A total of 174 compounds were identified or tentatively deduced from processed and unprocessed MOC samples according to MS data obtained in positive and negative ion mode. After MOC was processed with ginger juice, the peak areas of most phenolics increased, while the peak areas of most phenylethanoid glycosides decreased; as for neolignans, oxyneolignans, other lignans and alkaloids, changes in the peak area were variable, and the peak areas of terpenoid-lignans varied little. Additionally, gingerols and diarylheptanoids were only detected in the processed MOC sample. The contents of syringin, magnoloside A, and Magnoloside B decreased significantly in the processed MOC sample while no significant difference was observed in the contents of magnoflorine, magnocurarine, honokiol, obovatol, and magnolol. This study comprehensively explored the content variation of chemical components in processed and unprocessed MOC samples derived from different regions and with different tree ages using UPLC and UHPLC-Q-Orbitrap HRMS, and summarized the variation characteristics of various compounds. The results provide a data foundation for further research on pharmacodynamic substances of MOC processed with ginger juice.

Pharmacokinetics and metabolites of glycosides and lignans of the stem bark of Magnolia officinalis in functional dyspepsia and normal rats using liquid chromatography-tandem mass spectrometry.[Pubmed:35908283]

J Sep Sci. 2022 Oct;45(19):3663-3678.

The stem bark of Magnolia officinalis is a traditional Chinese medicine for the treatment of abdominal distention and functional dyspepsia. The pharmacokinetics of three glycosides (magnoloside A, Magnoloside B, and syringin) and two lignans (honokiol and magnolol) in both normal and functional dyspepsia rats were firstly investigated by ultra-performance liquid chromatography-triple quadrupole mass spectrometry method and the influences of the coexisting compounds on the pharmacokinetic parameters of honokiol and magnolol were also studied. It was found that all of the five target compounds were quickly absorbed and eliminated in both normal and functional dyspepsia rats, while, their residence time was significantly decreased in pathological states except magnoloside A. The coexisting compounds in the stem bark of M. officinalis significantly reduced absorption and increased elimination of honokiol in vivo. It's worth noticing that the volume of distribution of lignan was quite lower than that of a glycoside. Moreover, the metabolic profiling of magnoloside A, honokiol, and magnolol in vivo was analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method, from which three prototypes were identified and 35 metabolites were putatively characterized, and 18 unknown metabolites were reasonably characterized for the first time. The results indicated that sulfation and glucuronidation were the main metabolic pathways of honokiol and magnolol.

[Geo-herbalism study of Magnoliae Officinalis Cortex].[Pubmed:31602929]

Zhongguo Zhong Yao Za Zhi. 2019 Sep;44(17):3601-3607.

Magnoliae Officinalis Cortex( MOC),the stem bark of Magnolia officinalis( MO) and M. officinalis var. biloba( MOB),is a main ingredient in more than 200 types of Chinese formulae commonly used in clinics. MO and MOB are widely distributed in China,from Sichuan of the west to Zhejiang province of the east and from Shannxi province in the north to Guangxi province in the south. This review summarizes new findings on geo-heralism of MOC concerning textual research,plants taxonomy,genetic study,chemical study,and pharmacological activity,resulting in the following views. 1 in circleThe original plants of MOC are suggested to be divided into three geographic clans according to the form of leave and the result of genetic research; 2 in circleConcentrations of magnolol,honokiol,magnoloside A,Magnoloside B,magnoflorine,and beta-eudesmol in samples collected from different geographic areas are varied;3 in circleSamples of MOC produced in Hubei and Sichuan were traditionally regarded as Dao-di herbs,which were called Chuanpo,and the pure haplotype of MOC produced in Hubei may become a genetic index.

[Quality evaluation of Magnoliae Officinalis Cortex based on combinative method of fingerprint,quantitative analysis of multicomponents and chemometrics].[Pubmed:30989858]

Zhongguo Zhong Yao Za Zhi. 2019 Mar;44(5):975-982.

This study aims to establish a combinative method based on fingerprint,assay of multi-component and chemometrics for quality evaluation of Magnoliae Officinalis Cortex. Twenty batches of samples were determined by UPLC and a common mode of fingerprint was established. The similarities between fingerprints of 20 batches of samples were over 0. 90 and the common mode were evaluated. Eight components were identified as syringing, magnocurarine, magnoflorine, Magnoloside B, magnoloside A, honokiol,magnolol,and piperitylmagnolol by comparison with reference substances and their content in samples were simultaneously determined.Based on the results,the fingerprint had good consistency between the same origin and minor diversity between the different sources.Piperitylmagnolol and peak 13 could be used as a distinction with the different sources. According to content of 8 components,Fisher discriminant analysis model was established and different source sample was classified pursuant to the discriminant fraction. It is indicated that simultaneous quantification of multi components coupled with chemometrics analysis could be a well-acceptable strategy to identify and evaluate the quality of Magnoliae Officinalis Cortex.

Rapid purification of antioxidants from Magnolia officinalis by semi-prep-HPLC with a two-step separation strategy guided by on-line HPLC-radical scavenging detection.[Pubmed:30316138]

J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Nov 15;1100-1101:140-147.

A rapid and accurate strategy of screening, identification, and purification of antioxidants from natural medicines was put forward in this work, and it was applied to discover the antioxidants from magnolia officinals Rahd. et Wils. On-line HPLC-DPPH combined with electrospray ionization time-of-flight mass spectrometry was employed for the rapid screening and potential identification of the antioxidants. Semi-prep-HPLC with two-step separation procedure was employed for the separation and purification of the representative compounds (the first step: compounds 1-4 were obtained by gradient elution; the second step: compounds 5-7 were obtained by isocratic elution). NMR spectroscopy, mass spectrometry, and UV spectroscopy were used for further structural identification, and the activity of the compounds was verified by in vitro assay. With this method, 28 compounds with antioxidant activity were discovered in the extraction. After the preparation and purification, seven compounds with the purity over 95% were get, which were identified as syringing, Magnoloside B, magnoloside A, magnoloside F, magnolol, obvatol and honokiol. The results of in vitro assay showed that these seven compounds all had higher DPPH scavenging activity. Thus, all the results suggested that this work provide a more rapid, accurate and efficient methodology to achieve the screening, characterization and preparation of antioxidative constituents from complex natural products under active guidance.

An HPLC-DAD method for simultaneous quantitative determination of four active hydrophilic compounds in Magnoliae officinalis cortex.[Pubmed:25085894]

J Chromatogr Sci. 2015 Apr;53(4):598-602.

Magnoliae officinalis cortex (MOC), derived from Magnolia officinalis and its variation M. officinalis var. biloba, is an important traditional Chinese medicine. In our previous work, 11 hydrophilic ingredients of MOC were isolated and structurally elucidated and four, namely syringin (SG), magnoloside A (MA), Magnoloside B (MB) and magnoflorine (MF), showed bioactive effects. Herein, we describe an HPLC-DAD method for the simultaneous quantitative determination of MA, MB, MF and SG in MOC for the first time. The chromatographic separation of samples was performed on an Agilent Zorbax SB-C18 column (250 x 4.6 mm i.d., 5 microm) by gradient elution with water-acetic acid (pH 3.0) and methanol at a flow rate of 1.0 mL/min. The wavelengths were set at 265 nm for MF and SG, and 328 nm for MA and MB. The average recovery of the four compounds was from 97.63 to 103.84%. Nearly 100 MOC samples harvested from eight habitats were analyzed in which the contents of the tested compound varied in the range of 0.016-0.350% (MF), 0.010-0.337% (SG), 0.017-3.009% (MB) and 0.077-2.529% (MA). The analysis also indicated that MOC contains a significant amount of phenylethanoid glycosides. This was an unexpected finding because previously lignan was considered to be the main component of MOC.

Chemical differentiation of Da-Cheng-Qi-Tang, a Chinese medicine formula, prepared by traditional and modern decoction methods using UPLC/Q-TOFMS-based metabolomics approach.[Pubmed:23685412]

J Pharm Biomed Anal. 2013 Sep;83:34-42.

In order to evaluate chemical consistency between traditional and modern decoctions of Da-Cheng-Qi-Tang (DCQT), a classical Chinese medicine formula commonly used in the treatment of digestive diseases, an ultra performance liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOFMS) combined with multivariate statistical analysis was established to globally characterize the chemical profile and discover differentiating chemical markers. Two kinds of decoctions, namely traditional decoction (multi-step decoction of constituent herbs), and modern decoction (one-step decoction of all herbs), were prepared and subjected to UPLC-MS analysis, the datasets of tR-m/z pairs, ion intensities and sample codes were processed with supervised orthogonal partial least squared discriminant analysis (OPLS-DA) to comprehensively compare the chemical difference between these two kinds of decoction samples. The global chemical difference was found between traditional and modern decoctions, and rhein, sennoside A/B, diosmetin, Magnoloside B and naringin were the components contributing most to these differences. Based on the fact that traditional decoction of DCQT presents the higher concentration of rhein and sennoside A/B, mainly contributed to laxative activity of DCQT, the purgative effect of traditional decoction might be more potent, compared with modern decoction. However, the comparative study on purgative effect of traditional and modern DCQT remains to be further investigated using pharmacological approaches. Our findings also provide the early scientific evidence of traditional decoction method of DCQT.