NHS-Biotin

Biotinyltation reagent CAS# 35013-72-0

NHS-Biotin

Catalog No. BCC3577----Order now to get a substantial discount!

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Chemical structure

NHS-Biotin

3D structure

Chemical Properties of NHS-Biotin

Cas No. 35013-72-0 SDF Download SDF
PubChem ID 434213 Appearance Powder
Formula C14H19N3SO5 M.Wt 341.38
Type of Compound N/A Storage Desiccate at -20°C
Synonyms Biotin-NHS; Biotin N-hydroxysuccinimide ester; NHS-Biotin
Solubility >17.1mg/mL in DMSO
Chemical Name (2,5-dioxopyrrolidin-1-yl) 5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoate
SMILES C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
Standard InChIKey YMXHPSHLTSZXKH-UHFFFAOYSA-N
Standard InChI InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of NHS-Biotin

DescriptionNHS-Biotin is the simplest NHS-ester activated biotinylation reagent used for intracellular labeling.
Targetsintracellular proteins    

Protocol

Cell experiment: [1]

Cell lines

Bone marrow macrophages (BMMs)

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Reacting condition

180 μg/ml, 45 min

Applications

Cells were washed in PBS-0.1M HEPES, and cell surface proteins were biotinylated using NHS-Biotin. (450 µg NHS-biotin in 2.5 mL PBS-0.1 M HEPES per 150 mm2 plate, and then incubated for 45 minutes at room temperature). Cells were washed gently 3 times with cold PBS and then lysed in lysis buffer (10 mM Tris, 150 mM NaCl, 1.0 mM CaCl, 0.02% NaN3 2×protease inhibitor cocktail, and 2% Renex). Protein concentrations in the lysates were determined by bicinchoninic acid reaction, and equal amounts of total protein were immunoprecipitated.

Animal experiment:

Animal models

Dosage form

Application

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] McHugh K P, Hodivala-Dilke K, Zheng M H, et al. Mice lacking β3 integrins are osteosclerotic because of dysfunctional osteoclasts. Journal of Clinical Investigation, 2000, 105(4): 433-440.

NHS-Biotin Dilution Calculator

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NHS-Biotin Molarity Calculator

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Preparing Stock Solutions of NHS-Biotin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.9293 mL 14.6464 mL 29.2929 mL 58.5857 mL 73.2322 mL
5 mM 0.5859 mL 2.9293 mL 5.8586 mL 11.7171 mL 14.6464 mL
10 mM 0.2929 mL 1.4646 mL 2.9293 mL 5.8586 mL 7.3232 mL
50 mM 0.0586 mL 0.2929 mL 0.5859 mL 1.1717 mL 1.4646 mL
100 mM 0.0293 mL 0.1465 mL 0.2929 mL 0.5859 mL 0.7323 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on NHS-Biotin

NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.

NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

 Ref:

  1. Fouassier, L., Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.
  2. Nunomura, W., Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.
  3. Chiu, N.H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954–1959..
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References on NHS-Biotin

Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate.[Pubmed:17968972]

J Mass Spectrom. 2008 Mar;43(3):360-70.

NHS-Biotin modification as a specific lysine probe coupled to mass spectrometry detection is increasingly used over the past years for assessing amino acid accessibility of proteins or complexes as an alternative when well-established methods are challenged. We present a strategy based on usage in parallel of three commercially available reagents (Sulfo-NHS-Biotin, Sulfo-NHS-LC-biotin, and Sulfo-NHS-LC-LC-biotin) to efficiently assess the solvent accessibility of amino acids using MALDI-TOF mass spectrometry. The same qualitative pattern of reactivity was observed for these three reagents on the THUMPalpha protein at four reagent/polypeptide molar ratios (2 : 1, 6 : 1, 13 : 1, and 26 : 1). Peptide assignment of the detected ions gains in accuracy because of the triple redundancy due to specific increments of monoisotopic mass. These reagents are a good alternative to isotope labeling when using only a single MALDI-TOF mass spectrometer. We observed that hydroxyl groups of serine and tyrosine residues were also modified by these Sulfo-NHS-Biotin reagents. The low amount of protein required and the method's simplicity make this procedure accessible and affordable in order to obtain topological information on proteins difficult to purify. This method was used to identify two lysine residues of the TrmG10 methyltransferase from Pyrococcus abyssi that were differentially reactive, modified in the protein but not in the tRNA-protein complex.

The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method.[Pubmed:8578739]

Vox Sang. 1995;69(3):248-9.

Glycoprotein (Gp)IIb/IIIa molecules have been immunoprecipitated from platelets and leukocytes of cattle, goat, horse, human, sheep and swine using specific monoclonal antibodies. The sulfo-NHS-Biotin (sulfosuccinimidobiotin) method used to label the proteins has been found unsuitable for labelling the beta chain of the ruminant GPIIb/IIIa molecule. The beta chain was present on ruminant leukocytes and platelets when immunoprecipitates were silver stained.

Description

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

Keywords:

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