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Pieceid-2''-O-gallate

CAS# 105304-51-6

Pieceid-2''-O-gallate

2D Structure

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Pieceid-2''-O-gallate: 5mg $173 In Stock
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Quality Control of Pieceid-2''-O-gallate

3D structure

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Pieceid-2''-O-gallate

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Chemical Properties of Pieceid-2''-O-gallate

Cas No. 105304-51-6 SDF Download SDF
PubChem ID 6444105 Appearance Powder
Formula C27H26O12 M.Wt 542.5
Type of Compound Phenols Storage Desiccate at -20°C
Synonyms 3,4',5-trihydroxystilbene-4'-O-(2''-O-galloyl)glucopyranoside
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name [(2S,3R,4R,5S,6R)-5-[(E)-2-(3,5-dihydroxyphenyl)ethenyl]-4,5-dihydroxy-6-(hydroxymethyl)-2-phenoxyoxan-3-yl] 3,4,5-trihydroxybenzoate
SMILES C1=CC=C(C=C1)OC2C(C(C(C(O2)CO)(C=CC3=CC(=CC(=C3)O)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
Standard InChIKey YVAPPPYBCIJACC-UBFVITNGSA-N
Standard InChI InChI=1S/C27H26O12/c28-13-21-27(36,7-6-14-8-16(29)12-17(30)9-14)24(34)23(26(38-21)37-18-4-2-1-3-5-18)39-25(35)15-10-19(31)22(33)20(32)11-15/h1-12,21,23-24,26,28-34,36H,13H2/b7-6+/t21-,23-,24-,26-,27-/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Pieceid-2''-O-gallate

The roots of Pleuropterus ciliinervis

Biological Activity of Pieceid-2''-O-gallate

DescriptionPieceid-2''-O-gallate has antioxidant activity.
In vitro

Stilbenes from the roots of Pleuropterus ciliinervis and their antioxidant activities.[Reference: WebLink]

Phytochemistry, 2003, 64(3):759-763.


METHODS AND RESULTS:
Two stilbene glycosides, Pieceid-2''-O-gallate and pieceid-2″-O-coumarate, were isolated from the MeOH extract of the roots of Pleuropterus ciliinervis Nakai (Polygonaceae), together with two known compounds, resveratrol and pieceid. Their structures were determined spectroscopically, particularly by 2D NMR spectroscopic analysis. The antioxidant activities of stilbenes isolated were determined in vitro against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and by determining their lipid peroxidation inhibitory activities. Among the compounds isolated, pieceid-2″-O-gallate had the most potent inhibitory scavenging effect on DPPH, superoxide radicals and upon lipid peroxidation inhibition with IC50 values of 16.5, 23.9 and 5.1 μM, respectively.
CONCLUSIONS:
Two stilbene glycisides, (E)-pieceid-2″-O-gallate and (E)-pieceid 2″-O-cumarate, were isolated from the roots of P. ciliinervis, together with resveratrol and pieceid. They showed antioxidant activities.

Protocol of Pieceid-2''-O-gallate

Structure Identification
Química Nova,2014, 37(9):1465-1468.

Separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography.[Reference: WebLink]

Recebido em 26/03/2014; aceito em 08/07/2014; publicado na web em 22/09/2014 An effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established.
METHODS AND RESULTS:
In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of Pieceid-2''-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC).
CONCLUSIONS:
The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.

Pieceid-2''-O-gallate Dilution Calculator

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Pieceid-2''-O-gallate Molarity Calculator

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Preparing Stock Solutions of Pieceid-2''-O-gallate

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.8433 mL 9.2166 mL 18.4332 mL 36.8664 mL 46.0829 mL
5 mM 0.3687 mL 1.8433 mL 3.6866 mL 7.3733 mL 9.2166 mL
10 mM 0.1843 mL 0.9217 mL 1.8433 mL 3.6866 mL 4.6083 mL
50 mM 0.0369 mL 0.1843 mL 0.3687 mL 0.7373 mL 0.9217 mL
100 mM 0.0184 mL 0.0922 mL 0.1843 mL 0.3687 mL 0.4608 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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