(-)-Sativan

The Chemical Components of Sesbania grandiflora Root and Their Antituberculosis Activity.[Pubmed:24280680]

Pharmaceuticals (Basel). 2012 Aug 23;5(8):882-9.

Three isoflavanoids, isovestitol (1), medicarpin (2), and (-)-Sativan (3), along with another known compound, betulinic acid (4), were isolated from the root of Sesbania grandiflora. The structures of the isolated compounds were characterised by means of spectroscopic techniques (UV, IR, MS, 1H- and 13C-NMR, DEPT, COSY, HMQC, HMBC, and MS analysis). All the tested compounds 1-4 exhibited antituberculosis activity against Mycobacterium tuberculosis H37Rv, with MIC values of 50 microg/mL for compounds 1-3, and 100 microg/mL for compound 4, whereas, the methanol extract exhibited antituberculosis activity of 625 microg/mL. This is the first report on the occurrence of isoflavonoids in this plant and their antituberculosis activity.

The effect of sativan from Viola verecunda A. Gray on the expressions of matrix metalloproteinase-1 caused by ultraviolet irradiated cultured primary human skin fibroblasts.[Pubmed:16207520]

J Ethnopharmacol. 2006 Mar 8;104(1-2):12-7.

Matrix metalloproteinases (MMPs) are a family of enzymes whose main function is degradation of the extracellular matrix. Matrix metalloproteinase-1 (MMP-1) degrades type I procollagen constituting the major structural component of the basement membrane and extra cellular membrane. The enzymatic activity is found to be elevated in photoaging. With the aim of finding novel MMP-1 inhibitors from natural products, 15 extracts of the Viola taxa, which are used as prescriptions for skin eruption treatment in traditional medicine, were screened for their inhibitory activities towards MMP-1. Among the 15 tested materials, the methanol extracts of Viola hondoensis W. BECKER et H. BOISS, Viola ibukiana MAKINO, Viola lactiflora NAKAI and Viola verecunda A. GRAY were potential inhibitory to MMP-1, and other Viola taxa showed a weak inhibitory effect at a concentration of 100 microg/ml. We investigated the effect of MMP-1 inhibitory of the solvent fractions of the same plants (Viola hondoensis, Viola ibukiana and Viola verecunda). Therefore, a strong inhibition was found in the ethylacetate fractions of Viola verecunda with inhibitory activity (>90%) at a concentration of 10 microg/ml. Here we investigated the effect of (-)-Sativan isolated from the ethylacetate fractions of Viola verecunda on the expression of MMPs in UV-irradiated human skin fibroblasts in vitro. (-)-Sativan markedly reduced UV-induced MMP-1 expression at the protein levels in a dose-dependent manner. Our report is the first description for the ability of (-)-Sativan to regulate UV-induced MMP-1 expression.

Susceptibility of zoopathogenic fungi to phytoalexins.[Pubmed:7387136]

Antimicrob Agents Chemother. 1980 Feb;17(2):120-3.

Phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. They are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. Employing a broth dilution technique, we have found the zoopathogens Petriellidium boydii, Aspergillus flavus, A. fumigatus, Candida albicans, Coccidioides immitis, Cryptococcus neoformans, Histoplasma capsulatum, Rhizopus oryzae, Sporothrix schenckii, and Trichophyton rubrum to be inhibited by one or more of the phytoalexins maackiain, medicarpin, phaseollin, phaseollin isoflavan, pisatin, (-)-Sativan, and vestitol in concentrations of from 12.5 to 50 microgram/ml. Phaseollin isofalvan was the most effective of these. In agar cup diffusion tests growth of the yeast form of Blastomyces dermatitidis was inhibited by phaseollin at 50 microgram/ml and by phaseollin isoflavan at 25 microgram/ml. Susceptibility of the mycelial form of B. dermatitidis to these two compounds was demonstrated by inhibition of radial extension on agar plates.