TBTUpeptide coupling reagent CAS# 125700-67-6 |
- Nadifloxacin
Catalog No.:BCC4804
CAS No.:124858-35-1
- Calcipotriol monohydrate
Catalog No.:BCC1445
CAS No.:147657-22-5
- Halobetasol Propionate
Catalog No.:BCC4664
CAS No.:66852-54-8
- Dihydroartemisinin
Catalog No.:BCN6264
CAS No.:71939-50-9
Quality Control & MSDS
3D structure
Package In Stock
Number of papers citing our products
| Cas No. | 125700-67-6 | SDF | Download SDF |
| PubChem ID | 2733207 | Appearance | Powder |
| Formula | C11H16BF4N5O | M.Wt | 321.1 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Solubility | Soluble in water or 1% acetic acid | ||
| Chemical Name | [benzotriazol-1-yloxy(dimethylamino)methylidene]-dimethylazanium;tetrafluoroborate | ||
| SMILES | [B-](F)(F)(F)F.CN(C)C(=[N+](C)C)ON1C2=CC=CC=C2N=N1 | ||
| Standard InChIKey | JKEKMBGUVUKMQB-UHFFFAOYSA-N | ||
| Standard InChI | InChI=1S/C11H16N5O.BF4/c1-14(2)11(15(3)4)17-16-10-8-6-5-7-9(10)12-13-16;2-1(3,4)5/h5-8H,1-4H3;/q+1;-1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
TBTU Dilution Calculator
TBTU Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 3.1143 mL | 15.5715 mL | 31.1429 mL | 62.2859 mL | 77.8574 mL |
| 5 mM | 0.6229 mL | 3.1143 mL | 6.2286 mL | 12.4572 mL | 15.5715 mL |
| 10 mM | 0.3114 mL | 1.5571 mL | 3.1143 mL | 6.2286 mL | 7.7857 mL |
| 50 mM | 0.0623 mL | 0.3114 mL | 0.6229 mL | 1.2457 mL | 1.5571 mL |
| 100 mM | 0.0311 mL | 0.1557 mL | 0.3114 mL | 0.6229 mL | 0.7786 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
IC50: Not available.
The utilization of new peptide coupling reagents in organic synthesis has greatly flourished the development of peptide synthesis. TBTU, 2-(1H-Benzotriazol-1-yl)-1,1,3,3-tetramethyluronoium hexafluorphosphate, serves as a typical peptide coupling reagent which has a relatively lower racemization. In normal condition, coupling reactions mediated by TETU take only six minutes to complete when HOBt is added. Moreover, racemization in this reaction could be reduced to insignificant levels. Due to these features, TBTU is regarded as one of the key reagents of choice in both manufactory and lab. [1]
In vitro: It was reported that during synthesis of the macrocyclic peptide cyclotheonamide B, TBTU played an important role in coupling steps. Studies showed that TBTU had been successfully used in several coupling reactions, for instance, this reagent was suitable for couplings involving proline nitrogen, and therefore served as a crucial reagent for the macrolactamization. Although TBTU normally proceeded with little racemization, to suppress the racemization completely, HOBt was also required. Thus, in a typical reaction system, TBTU was added into 0.5 mM solution of CH2Cl2, followed by addition of HOBt and pyridine. Finally, cyclopentapeptide was obtained with a yield of 61%. [1]
In vivo: So far, no in vivo data has been reported.
Clinical trial: So far, no clinical trial has been conducted.
Reference:
[1]Bastiaans HM, van der Baan JL and Ottenheijm HC. Flexible and convergent total synthesis of cyclotheonamide B. J. Org. Chem. 1997; 62: 3880-9.
- (-)-Epicatechin gallate
Catalog No.:BCN6327
CAS No.:1257-08-5
- Lavendustin A
Catalog No.:BCN1808
CAS No.:125697-92-9
- Lavendustin B
Catalog No.:BCN1809
CAS No.:125697-91-8
- CDK4 inhibitor
Catalog No.:BCC4242
CAS No.:1256963-02-6
- 2''-O-acetyl-platyconic acid A
Catalog No.:BCN3318
CAS No.:1256935-30-4
- 3''-O-acetyl-platyconic acid A
Catalog No.:BCN3319
CAS No.:1256935-28-0
- Blinin
Catalog No.:BCN8455
CAS No.:125675-09-4
- CH5424802
Catalog No.:BCC3749
CAS No.:1256580-46-7
- Ledipasvir
Catalog No.:BCC1696
CAS No.:1256388-51-8
- RuBi-Nicotine
Catalog No.:BCC7793
CAS No.:1256362-30-7
- AI-10-49
Catalog No.:BCC3973
CAS No.:1256094-72-0
- TRX818
Catalog No.:BCC6458
CAS No.:1256037-58-7
- TDBTU
Catalog No.:BCC2825
CAS No.:125700-69-8
- TPTU
Catalog No.:BCC2827
CAS No.:125700-71-2
- ABT-199
Catalog No.:BCC3614
CAS No.:1257044-40-8
- TC-SP 14
Catalog No.:BCC7926
CAS No.:1257093-40-5
- TC-G 24
Catalog No.:BCC6146
CAS No.:1257256-44-2
- NPEC-caged-noradrenalin
Catalog No.:BCC7835
CAS No.:1257323-83-3
- NPEC-caged-(S)-AMPA
Catalog No.:BCC7789
CAS No.:1257323-84-4
- NPEC-caged-(S)-3,4-DCPG
Catalog No.:BCC7652
CAS No.:1257323-85-5
- NPEC-caged-serotonin
Catalog No.:BCC7836
CAS No.:1257326-22-9
- NPEC-caged-dopamine
Catalog No.:BCC7837
CAS No.:1257326-23-0
- GSK 789472 hydrochloride
Catalog No.:BCC7818
CAS No.:1257326-24-1
- TC-E 5006
Catalog No.:BCC7981
CAS No.:1257395-14-4
Occupational rhinitis and bronchial asthma due to TBTU and HBTU sensitization.[Pubmed:12968399]
J Investig Allergol Clin Immunol. 2003;13(2):133-4.
Exposure to an increasing amount of products in the work environment is leading to new cases of occupational asthma among workers. We report the case of a worker at a pharmaceutical plant who developed occupational rhinitis and bronchial asthma due to HBTU: 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate and TBTU: 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate sensitization, two chemical products widely used in peptide synthesis and coupling. Skin tests (prick test) with HBTU and TBTU solutions in PBS were positive at a concentration of 1 mg/ml. Skin tests with the same solutions in 10 atopic controls yielded a negative result. Nasal challenge tests with these products were positive with HBTU at a concentration of 0.01 mg/ml and TBTU at a concentration of 1 mg/ml. In both cases PNIF (peak nasal inspiratory flow) decreased by more than 60% and severe sneezing and rhinorrhea were induced. Nasal challenge tests performed on 10 atopic controls with TBTU and HBTU at a concentration of 1 mg/ml were negative. We conclude that the patient presents occupational rhinitis and bronchial asthma due to TBTU and HBTU; the operational mechanism is probably immunological IgE-mediated given the positive prick tests and nasal challenge with these products.
