25S-InokosteroneCAS# 19595-18-7 |
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| Cas No. | 19595-18-7 | SDF | Download SDF |
| PubChem ID | 12358616 | Appearance | Powder |
| Formula | C27H44O7 | M.Wt | 480.6 |
| Type of Compound | Steroids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| Chemical Name | (2S,3R,5R,9R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-[(2R,3R,6S)-2,3,7-trihydroxy-6-methylheptan-2-yl]-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one | ||
| SMILES | CC(CCC(C(C)(C1CCC2(C1(CCC3C2=CC(=O)C4C3(CC(C(C4)O)O)C)C)O)O)O)CO | ||
| Standard InChIKey | JQNVCUBPURTQPQ-BMZRUTLMSA-N | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
The roots of Achyranthes bidentata Blume.
| Description | 1. 25S-Inokosterone and 25R-inokosterone exhibit potent inhibition (80-95% at ) against TNF-expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells, they have excellent anti-atopy activity, thus they could be used to a large range of functional anti-atopy cosmetics. |
| Targets | TNF-α | IFN-γ |
25S-Inokosterone Dilution Calculator
25S-Inokosterone Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.0807 mL | 10.4037 mL | 20.8073 mL | 41.6146 mL | 52.0183 mL |
| 5 mM | 0.4161 mL | 2.0807 mL | 4.1615 mL | 8.3229 mL | 10.4037 mL |
| 10 mM | 0.2081 mL | 1.0404 mL | 2.0807 mL | 4.1615 mL | 5.2018 mL |
| 50 mM | 0.0416 mL | 0.2081 mL | 0.4161 mL | 0.8323 mL | 1.0404 mL |
| 100 mM | 0.0208 mL | 0.104 mL | 0.2081 mL | 0.4161 mL | 0.5202 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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High performance liquid chromatography used for quality control of Achyranthis Radix.[Pubmed:22941488]
Arch Pharm Res. 2012 Aug;35(8):1449-55.
To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-Inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J'sphere ODS C(18) column (250 mm x 4.6 mm, 4 mum) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix.
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