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6-Hydroxykaempferol 3-O-beta-D-glucoside

CAS# 145134-61-8

6-Hydroxykaempferol 3-O-beta-D-glucoside

2D Structure

Catalog No. BCN8836----Order now to get a substantial discount!

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6-Hydroxykaempferol 3-O-beta-D-glucoside: 5mg $322 In Stock
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Quality Control of 6-Hydroxykaempferol 3-O-beta-D-glucoside

3D structure

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6-Hydroxykaempferol 3-O-beta-D-glucoside

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Chemical Properties of 6-Hydroxykaempferol 3-O-beta-D-glucoside

Cas No. 145134-61-8 SDF Download SDF
PubChem ID 85469293 Appearance Powder
Formula C21H20O12 M.Wt 464.4
Type of Compound Flavonoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 5,6,7-trihydroxy-2-(4-hydroxyphenyl)-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one
SMILES C1=CC(=CC=C1C2=C(C(=O)C3=C(O2)C=C(C(=C3O)O)O)OC4C(C(C(C(O4)CO)O)O)O)O
Standard InChIKey DIYGQKBUNSAYQA-CZTZGLBASA-N
Standard InChI InChI=1S/C21H20O12/c22-6-11-14(26)17(29)18(30)21(32-11)33-20-16(28)12-10(5-9(24)13(25)15(12)27)31-19(20)7-1-3-8(23)4-2-7/h1-5,11,14,17-18,21-27,29-30H,6H2/t11-,14-,17+,18-,21+/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 6-Hydroxykaempferol 3-O-beta-D-glucoside

The herbs of Chrysactinia tinctorius

Biological Activity of 6-Hydroxykaempferol 3-O-beta-D-glucoside

Description6-Hydroxykaempferol 3-O-beta-D-glucoside has anti-thrombotic,and antioxidative activities.
In vitro

Separation and evaluation of antioxidant constituents from Carthamus tinctorius.[Pubmed: 25522615]

Zhongguo Zhong Yao Za Zhi. 2014 Sep;39(17):3295-300.

Bio-active components from Carthamus tinctorius were separated on the basis of antioxidant capacities in vitro.
METHODS AND RESULTS:
The antioxidant capacity was investigated on the basis of the ability to scavenge DPPH radical, ABTS radical and reduce Fe3+ of different polar fractions. Furthermore, the chemical compounds were isolated from bio-active fraction, and were evaluated for the antioxidative effects. Five major components were isolated and identified from water extract as 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside(1), 6-hydroxykaempferol 3-O-β-rutinoside-6-O-β-D-glucoside (2) 6-hydroxykaempferol 3-O-β-D-glucoside (6-Hydroxykaempferol 3-O-beta-D-glucoside,3), hydroxysafflor yellow A (4) and anhydrosafflor yellow B (5).
CONCLUSIONS:
By evaluating and comparing the antioxidative effects of different fractions and obtained compounds, the results showed that water extract displayed significantly high antioxidative activities and 6-hydroxykaempferol glycosides and quinochalcone C-glycosides were found as main contribution for antioxidant property.

Protocol of 6-Hydroxykaempferol 3-O-beta-D-glucoside

Structure Identification
Molecules. 2019 Sep 17;24(18). pii: E3381.

The Comprehensive Evaluation of Safflowers in Different Producing Areas by Combined Analysis of Color, Chemical Compounds, and Biological Activity.[Pubmed: 31533325 ]

In the present study, a new strategy including the combination of external appearance, chemical detection, and biological analysis was proposed for the comprehensive evaluation of safflowers in different producing areas.
METHODS AND RESULTS:
Firstly, 40 batches of safflower samples were classified into class I and II based on color measurements and K-means clustering analysis. Secondly, a rapid and sensitive analytical method was developed for simultaneous quantification of 16 chromaticity-related characteristic components (including characteristic components hydroxysafflor yellow A, anhydrosafflor yellow B, safflomin C, and another 13 flavonoid glycosides) in safflowers by ultra-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UPLC-QTRAP®/MS2). The results of the quantification indicate that hydroxysafflor yellow A, anhydrosafflor yellow B, kaempferol, quercetin, and safflomin C had significant differences between the two types of safflower, and class I of safflower had a higher content of hydroxysafflor yellow A, anhydrosafflor yellow B, and safflomin C as the main anti-thrombotic components in safflower. Thirdly, chemometrics methods were employed to illustrate the relationship in multivariate data of color measurements and chromaticity-related characteristic components. As a result, kaempferol-3-O-rutinoside and 6-hydroxykaempferol-3-O-β-d-glucoside(6-Hydroxykaempferol 3-O-beta-D-glucoside) were strongly associated with the color indicators. Finally, anti-thrombotic analysis was used to evaluate activity and verify the suitability of the classification basis of safflower based on the color measurements. It was shown that brighter, redder, yellower, more orange-yellow, and more vivid safflowers divided into class I had a higher content of characteristic components and better anti-thrombotic activity.
CONCLUSIONS:
In summary, the presented strategy has potential for quality evaluation of other flower medicinal materials.

6-Hydroxykaempferol 3-O-beta-D-glucoside Dilution Calculator

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6-Hydroxykaempferol 3-O-beta-D-glucoside Molarity Calculator

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Preparing Stock Solutions of 6-Hydroxykaempferol 3-O-beta-D-glucoside

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.1533 mL 10.7666 mL 21.5332 mL 43.0663 mL 53.8329 mL
5 mM 0.4307 mL 2.1533 mL 4.3066 mL 8.6133 mL 10.7666 mL
10 mM 0.2153 mL 1.0767 mL 2.1533 mL 4.3066 mL 5.3833 mL
50 mM 0.0431 mL 0.2153 mL 0.4307 mL 0.8613 mL 1.0767 mL
100 mM 0.0215 mL 0.1077 mL 0.2153 mL 0.4307 mL 0.5383 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 6-Hydroxykaempferol 3-O-beta-D-glucoside

[Separation and evaluation of antioxidant constituents from Carthamus tinctorius].[Pubmed:25522615]

Zhongguo Zhong Yao Za Zhi. 2014 Sep;39(17):3295-300.

Bio-active components from Carthamus tinctorius were separated on the basis of antioxidant capacities in vitro. The antioxidant capacity was investigated on the basis of the ability to scavenge DPPH radical, ABTS radical and reduce Fe3+ of different polar fractions. Furthermore, the chemical compounds were isolated from bio-active fraction, and were evaluated for the antioxidative effects. Five major components were isolated and identified from water extract as 6-hydroxykaempferol 3,6,7-tri-O-beta-D-glucoside(1), 6-hydroxykaempferol 3-O-beta-rutinoside-6-O-beta-D-glucoside (2), 6-Hydroxykaempferol 3-O-beta-D-glucoside (3), hydroxysafflor yellow A (4) and anhydrosafflor yellow B (5). By evaluating and comparing the antioxidative effects of different fractions and obtained compounds, the results showed that water extract displayed significantly high antioxidative activities and 6-hydroxykaempferol glycosides and quinochalcone C-glycosides were found as main contribution for antioxidant property.

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