CK 666Arp2/3 inhibitor; inhibits actin polymerization CAS# 442633-00-3 |
- Narciclasine
Catalog No.:BCN4732
CAS No.:29477-83-6
Quality Control & MSDS
Number of papers citing our products
Chemical structure
3D structure
Cas No. | 442633-00-3 | SDF | Download SDF |
PubChem ID | 589075 | Appearance | Powder |
Formula | C18H17FN2O | M.Wt | 296.34 |
Type of Compound | N/A | Storage | Desiccate at -20°C |
Solubility | DMSO : 125 mg/mL (421.81 mM; Need ultrasonic) | ||
Chemical Name | 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide | ||
SMILES | CC1=C(C2=CC=CC=C2N1)CCNC(=O)C3=CC=CC=C3F | ||
Standard InChIKey | UXRKUKRXVWJFER-UHFFFAOYSA-N | ||
Standard InChI | InChI=1S/C18H17FN2O/c1-12-13(14-6-3-5-9-17(14)21-12)10-11-20-18(22)15-7-2-4-8-16(15)19/h2-9,21H,10-11H2,1H3,(H,20,22) | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | Inhibitor of the Arp2/3 complex; inhibits actin polymerization (IC50 = 4 μM). |
CK 666 Dilution Calculator
CK 666 Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 3.3745 mL | 16.8725 mL | 33.745 mL | 67.49 mL | 84.3626 mL |
5 mM | 0.6749 mL | 3.3745 mL | 6.749 mL | 13.498 mL | 16.8725 mL |
10 mM | 0.3375 mL | 1.6873 mL | 3.3745 mL | 6.749 mL | 8.4363 mL |
50 mM | 0.0675 mL | 0.3375 mL | 0.6749 mL | 1.3498 mL | 1.6873 mL |
100 mM | 0.0337 mL | 0.1687 mL | 0.3375 mL | 0.6749 mL | 0.8436 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
- CK-636
Catalog No.:BCC5107
CAS No.:442632-72-6
- Aspertine C
Catalog No.:BCN2153
CAS No.:442155-62-6
- Clemizole
Catalog No.:BCC1485
CAS No.:442-52-4
- Harmine
Catalog No.:BCN5494
CAS No.:442-51-3
- Macitentan
Catalog No.:BCC1142
CAS No.:441798-33-0
- 6-Acetylacteoside
Catalog No.:BCC8109
CAS No.:441769-43-3
- 2-(Cyanomethyl)benzimidazole
Catalog No.:BCC8483
CAS No.:4414-88-4
- Eribulin mesylate
Catalog No.:BCC5173
CAS No.:441045-17-6
- Benzoin oxime
Catalog No.:BCC8858
CAS No.:441-38-3
- 3-Phenyl-2-propen-1-ol
Catalog No.:BCN5493
CAS No.:4407-36-7
- WAY 200070
Catalog No.:BCC7669
CAS No.:440122-66-7
- K 579
Catalog No.:BCC2364
CAS No.:440100-64-1
- JW 67
Catalog No.:BCC6251
CAS No.:442644-28-2
- Anguizole
Catalog No.:BCC1365
CAS No.:442666-98-0
- Methyl isodrimeninol
Catalog No.:BCN5495
CAS No.:442851-27-6
- Tabersonine
Catalog No.:BCN5496
CAS No.:4429-63-4
- Metronidazole
Catalog No.:BCC9046
CAS No.:443-48-1
- Ligustilide
Catalog No.:BCN1031
CAS No.:4431-01-0
- Isopedicin
Catalog No.:BCN4633
CAS No.:4431-42-9
- SF 11
Catalog No.:BCC7805
CAS No.:443292-81-7
- Z-DL-Met-OH
Catalog No.:BCC2759
CAS No.:4434-61-1
- BC 11 hydrobromide
Catalog No.:BCC2381
CAS No.:443776-49-6
- JNJ-7706621
Catalog No.:BCC2171
CAS No.:443797-96-4
- 3-Chloro-4-(3-fluorobenzyloxy)nitrobenzene
Catalog No.:BCC8626
CAS No.:443882-99-3
Small molecules CK-666 and CK-869 inhibit actin-related protein 2/3 complex by blocking an activating conformational change.[Pubmed:23623350]
Chem Biol. 2013 May 23;20(5):701-12.
Actin-related protein 2/3 (Arp2/3) complex is a seven-subunit assembly that nucleates branched actin filaments. Small molecule inhibitors CK-666 and CK-869 bind to Arp2/3 complex and inhibit nucleation, but their modes of action are unknown. Here, we use biochemical and structural methods to determine the mechanism of each inhibitor. Our data indicate that CK-666 stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation, while CK-869 binds to a serendipitous pocket on Arp3 and allosterically destabilizes the short pitch Arp3-Arp2 interface. These results provide key insights into the relationship between conformation and activity in Arp2/3 complex and will be critical for interpreting the influence of the inhibitors on actin filament networks in vivo.
Characterization of two classes of small molecule inhibitors of Arp2/3 complex.[Pubmed:19648907]
Nature. 2009 Aug 20;460(7258):1031-4.
Polymerization of actin filaments directed by the actin-related protein (Arp)2/3 complex supports many types of cellular movements. However, questions remain regarding the relative contributions of Arp2/3 complex versus other mechanisms of actin filament nucleation to processes such as path finding by neuronal growth cones; this is because of the lack of simple methods to inhibit Arp2/3 complex reversibly in living cells. Here we describe two classes of small molecules that bind to different sites on the Arp2/3 complex and inhibit its ability to nucleate actin filaments. CK-0944636 binds between Arp2 and Arp3, where it appears to block movement of Arp2 and Arp3 into their active conformation. CK-0993548 inserts into the hydrophobic core of Arp3 and alters its conformation. Both classes of compounds inhibit formation of actin filament comet tails by Listeria and podosomes by monocytes. Two inhibitors with different mechanisms of action provide a powerful approach for studying the Arp2/3 complex in living cells.