Cornin

CAS# 548-37-8

Cornin

Catalog No. BCN5008----Order now to get a substantial discount!

Product Name & Size Price Stock
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Quality Control of Cornin

Number of papers citing our products

Chemical structure

Cornin

3D structure

Chemical Properties of Cornin

Cas No. 548-37-8 SDF Download SDF
PubChem ID 73467 Appearance White powder
Formula C17H24O10 M.Wt 388.37
Type of Compound Iridoids Storage Desiccate at -20°C
Synonyms Cornin; Verbenaloside
Solubility DMSO : 250 mg/mL (643.72 mM; Need ultrasonic)
Chemical Name methyl (1S,4aS,7S,7aR)-7-methyl-5-oxo-1-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4a,6,7,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylate
SMILES CC1CC(=O)C2C1C(OC=C2C(=O)OC)OC3C(C(C(C(O3)CO)O)O)O
Standard InChIKey HLXRWTJXGMHOFN-XJSNKYLASA-N
Standard InChI InChI=1S/C17H24O10/c1-6-3-8(19)11-7(15(23)24-2)5-25-16(10(6)11)27-17-14(22)13(21)12(20)9(4-18)26-17/h5-6,9-14,16-18,20-22H,3-4H2,1-2H3/t6-,9+,10+,11-,12+,13-,14+,16-,17-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Cornin

1 Verbena sp.

Biological Activity of Cornin

DescriptionVerbenalin has been reported to exhibit uterine stimulant activity and demonstrated cardioprotection against experimental myocardial ischemic injury and cerebral ischemia injury. Cornin induces angiogenesis in vitro via a programmed PI3K/Akt/eNOS/VEGF signaling axis, it also has antimitotic action on dividing cell.
TargetsPI3K | Akt | NOS | VEGFR | ROS
In vitro

Purification and some characteristics of liver cytosol cornin, an antimitotic substance from rat liver cytosol.[Pubmed: 144419]

Acta Med Okayama. 1977 Jun;31(3):203-9.

Further purification and characterization are reported on rat cytosol Cornin (RLCC), an antimitotic substance.
METHODS AND RESULTS:
Fraction I (purified RLCC) was purified more than 10-fold from crude RLCC with Sephadex G-50 column chromatography and showed a remarkable inhibitory effect on division of inseminated sea urchin eggs and mouse fibroblast cells. Fraction I was observed as one spot, and the molecular weight was estimated to be about 25,000 by thin layer gel filtration. Fraction I contained protein (92%) and RNA (8%), but the antimitotic activity was scarcely affected by treatment by pancreatic RNase. The protein of Fraction I was separated into two bands by SDS-polyacrylamide gel electrophoresis, and the molecular weight was estimated as 10,000 and 15,000, respectively.
CONCLUSIONS:
The 50% inhibition dose of Fraction I on the first division of inseminated sea urchin eggs and on proliferation of mouse L cells was about 2.5 X 10(-5) g/ml and 5 X 10(-4) g/ml, respectively. The yield of fraction I was about 35 mg from 100 g rat liver.

Antimitotic action of cornin as a biologically active polypeptide. I. Biochemical properties of cornin.[Reference: WebLink]

Acta Medicinae Okayama, 1965, 19(1):11-8.

Antimitotic action of Cornin as a biologically active polypeptide. I. Biochemical properties of Cornin.

In vivo

Cornin ameliorates cerebral infarction in rats by antioxidant action and stabilization of mitochondrial function.[Pubmed: 20041427]

Phytother Res. 2010 Apr;24(4):547-52.

This study was conducted to investigate the efficacy of Cornin, an iridoid glycoside, in an experimental cerebral ischemia induced by middle cerebral artery occlusion (MCAO) and reperfusion (I/R), and to elucidate the potential mechanism.
METHODS AND RESULTS:
Adult male Sprague-Dawley rats were subjected to MCAO for 1 h, then reperfusion for 23 h. Behavioral tests were used to evaluate the damage to central nervous system. The cerebral infarct volume and histopathological damage were assessed to evaluate the brain pathophysiological changes. Spectrophotometric assay methods were used to determine the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GPx). Contents of malondialdehyde (MDA), the generation of reactive oxygen species (ROS) as well as respiratory control ratio and respiratory enzymes of the brain mitochondria were also determined. The results showed that Cornin significantly decreased neurological deficit scores, and reduced cerebral infarct volume and degenerative neurons. Meanwhile, Cornin significantly increased the brain ATP content, improved mitochondrial energy metabolism, inhibited the elevation of MDA content and ROS generation, and attenuated the decrease of SOD and GPx activities in brain mitochondria.
CONCLUSIONS:
These findings indicate that Cornin has protective potential against cerebral ischemia injury and its protective effects may be due to amelioration of cerebral mitochondrial function and its antioxidant property.

Cardioprotection against experimental myocardial ischemic injury using cornin.[Pubmed: 26871971 ]

Braz J Med Biol Res. 2016 Feb;49(2):e5039.

Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside Cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro.
METHODS AND RESULTS:
Effects of Cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with Cornin (10 µM) blocked the reduction of expression of phospho-CREB and phospho-Akt in a hypoxic condition. Treatment of rats with Cornin (30 mg/kg, iv) protected them from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics, and reduction of severity of myocardial damage. Cornin treatment also attenuated the reduction of expression of phospho-CREB and phospho-Akt in ischemic myocardial tissue.
CONCLUSIONS:
These data suggest that Cornin exerts protective effects due to an increase in expression of phospho-CREB and phospho-Akt.

Protocol of Cornin

Kinase Assay

Cornin induces angiogenesis through PI3K-Akt-eNOS-VEGF signaling pathway.[Pubmed: 23702325]

Food Chem Toxicol. 2013 Aug;58:340-6.

In the present study, we sought to elucidate whether Cornin contributes to induce angiogenesis and its mechanisms. To this end, we examined the role of Cornin on human brain microvascular endothelial cell line (HBMEC) proliferation, invasion, and tube formation in in vitro.
METHODS AND RESULTS:
For study of mechanism, the phosphoinositide 3 kinase (PI3K)-Akt inhibitor LY294002, endothelial nitric oxide synthase (eNOS) inhibitor L-NAME, vascular endothelial growth factor (VEGF) antagonist sFlt-1 and VEGF receptor blocker SU-1498 were used. HMBEC proliferation was tested by MTT. Scratch adhesion test was used to assess the ability of invasion. A matrigel tube formation assay was performed to test capillary tube formation ability. PI3K-Akt-eNOS-VEGF pathway activation in HMBEC was tested by Western blot.
CONCLUSIONS:
Our data suggested that Cornin induces angiogenesis in vitro by increasing proliferation, invasion and tube formation. VEGF expression was increasing by Cornin and counteracted by VEGF antagonist sFlt-1, LY294002 and L-NAME in HMBEC. Tube formation was increased by Cornin and counteracted by VEGF receptor blocker-SU1498, LY294002 and L-NAME. It may be suggested that Cornin induces angiogenesis in vitro via a programmed PI3K/Akt/eNOS/VEGF signaling axis.

Cornin Dilution Calculator

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Cornin Molarity Calculator

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Preparing Stock Solutions of Cornin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.5749 mL 12.8743 mL 25.7486 mL 51.4973 mL 64.3716 mL
5 mM 0.515 mL 2.5749 mL 5.1497 mL 10.2995 mL 12.8743 mL
10 mM 0.2575 mL 1.2874 mL 2.5749 mL 5.1497 mL 6.4372 mL
50 mM 0.0515 mL 0.2575 mL 0.515 mL 1.0299 mL 1.2874 mL
100 mM 0.0257 mL 0.1287 mL 0.2575 mL 0.515 mL 0.6437 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Cornin

Purification and some characteristics of liver cytosol cornin, an antimitotic substance from rat liver cytosol.[Pubmed:144419]

Acta Med Okayama. 1977 Jun;31(3):203-9.

Further purification and characterization are reported on rat cytosol Cornin (RLCC), an antimitotic substance. Fraction I (purified RLCC) was purified more than 10-fold from crude RLCC with Sephadex G-50 column chromatography and showed a remarkable inhibitory effect on division of inseminated sea urchin eggs and mouse fibroblast cells. Fraction I was observed as one spot, and the molecular weight was estimated to be about 25,000 by thin layer gel filtration. Fraction I contained protein (92%) and RNA (8%), but the antimitotic activity was scarcely affected by treatment by pancreatic RNase. The protein of Fraction I was separated into two bands by SDS-polyacrylamide gel electrophoresis, and the molecular weight was estimated as 10,000 and 15,000, respectively. The 50% inhibition dose of Fraction I on the first division of inseminated sea urchin eggs and on proliferation of mouse L cells was about 2.5 X 10(-5) g/ml and 5 X 10(-4) g/ml, respectively. The yield of fraction I was about 35 mg from 100 g rat liver.

Cornin ameliorates cerebral infarction in rats by antioxidant action and stabilization of mitochondrial function.[Pubmed:20041427]

Phytother Res. 2010 Apr;24(4):547-52.

This study was conducted to investigate the efficacy of Cornin, an iridoid glycoside, in an experimental cerebral ischemia induced by middle cerebral artery occlusion (MCAO) and reperfusion (I/R), and to elucidate the potential mechanism. Adult male Sprague-Dawley rats were subjected to MCAO for 1 h, then reperfusion for 23 h. Behavioral tests were used to evaluate the damage to central nervous system. The cerebral infarct volume and histopathological damage were assessed to evaluate the brain pathophysiological changes. Spectrophotometric assay methods were used to determine the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GPx). Contents of malondialdehyde (MDA), the generation of reactive oxygen species (ROS) as well as respiratory control ratio and respiratory enzymes of the brain mitochondria were also determined. The results showed that Cornin significantly decreased neurological deficit scores, and reduced cerebral infarct volume and degenerative neurons. Meanwhile, Cornin significantly increased the brain ATP content, improved mitochondrial energy metabolism, inhibited the elevation of MDA content and ROS generation, and attenuated the decrease of SOD and GPx activities in brain mitochondria. These findings indicate that Cornin has protective potential against cerebral ischemia injury and its protective effects may be due to amelioration of cerebral mitochondrial function and its antioxidant property.

Cardioprotection against experimental myocardial ischemic injury using cornin.[Pubmed:26871971]

Braz J Med Biol Res. 2016 Feb;49(2):e5039.

Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside Cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of Cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with Cornin (10 microM) blocked the reduction of expression of phospho-CREB and phospho-Akt in a hypoxic condition. Treatment of rats with Cornin (30 mg/kg, iv) protected them from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics, and reduction of severity of myocardial damage. Cornin treatment also attenuated the reduction of expression of phospho-CREB and phospho-Akt in ischemic myocardial tissue. These data suggest that Cornin exerts protective effects due to an increase in expression of phospho-CREB and phospho-Akt.

Cornin induces angiogenesis through PI3K-Akt-eNOS-VEGF signaling pathway.[Pubmed:23702325]

Food Chem Toxicol. 2013 Aug;58:340-6.

In the present study, we sought to elucidate whether Cornin contributes to induce angiogenesis and its mechanisms. To this end, we examined the role of Cornin on human brain microvascular endothelial cell line (HBMEC) proliferation, invasion, and tube formation in in vitro. For study of mechanism, the phosphoinositide 3 kinase (PI3K)-Akt inhibitor LY294002, endothelial nitric oxide synthase (eNOS) inhibitor L-NAME, vascular endothelial growth factor (VEGF) antagonist sFlt-1 and VEGF receptor blocker SU-1498 were used. HMBEC proliferation was tested by MTT. Scratch adhesion test was used to assess the ability of invasion. A matrigel tube formation assay was performed to test capillary tube formation ability. PI3K-Akt-eNOS-VEGF pathway activation in HMBEC was tested by Western blot. Our data suggested that Cornin induces angiogenesis in vitro by increasing proliferation, invasion and tube formation. VEGF expression was increasing by Cornin and counteracted by VEGF antagonist sFlt-1, LY294002 and L-NAME in HMBEC. Tube formation was increased by Cornin and counteracted by VEGF receptor blocker-SU1498, LY294002 and L-NAME. It may be suggested that Cornin induces angiogenesis in vitro via a programmed PI3K/Akt/eNOS/VEGF signaling axis.

Description

Verbenalin is Verbena glycoside, with anti-inflammatory, anti-fungal anti-virus activities. Verbenalin has a good effect on prostatitis. Verbenalin can reduce cerebral ischemia-reperfusion injury.

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