Endothelin 3 (human, rat)

The role of endothelin in the control of adrenocortical function: stimulation of endothelin release by ACTH and the effects of endothelin-1 and endothelin-3 on steroidogenesis in rat and human adrenocortical cells.[Pubmed:1848586]

J Endocrinol. 1991 Feb;128(2):275-80.

The rate of blood flow through the intact adrenal gland is closely linked to steroid hormone secretion, and although the mechanism involved is unknown, it is thought to involve secretory products of the vascular endothelium. In dispersed cell preparations, endothelin-1 and -3 both caused a dose-dependent and highly sensitive increase in steroid secretion by zona glomerulosa and zona fasciculata cells of the rat and human adrenal cortex. In addition, when the perfused rat adrenal was stimulated with ACTH, significant increases in steroid secretion and perfusion medium flow rate were accompanied by significantly increased secretion of immunoreactive endothelin into the adrenal vein. It is proposed that endothelin has a role in mediating the adrenocortical response to ACTH stimulation.

Involvement of ET(A) and ET(B) receptors in the activation of phospholipase D by endothelins in cultured rat cortical astrocytes.[Pubmed:9756390]

Br J Pharmacol. 1998 Aug;124(8):1728-34.

This study was performed to characterize the receptor subtypes involved in the endothelin stimulation of phospholipase D (PLD) in rat cortical astrocytes in primary culture. PLD activity was determined by measuring the formation of [32P]phosphatidylbutanol in [32P]orthophosphate prelabelled cells stimulated in the presence of 25 mM butanol. The agonists endothelin-1 (ET-1), endothelin-3 (ET-3), sarafotoxin 6c (S6c) and IRL 1620 elicited PLD activation in a concentration-dependent manner. The potencies of ET-1, ET-3 and S6c were similar. The maximal effects evoked by the ET(B)-preferring agonists, ET-3, S6c and IRL 1620, were significantly lower than the maximal response to the non-selective agonist ET-1. The response to 1 nM ET-1 was inhibited by increasing concentrations of the ET(A) receptor antagonist BQ-123 in a biphasic manner. A high potency component of the inhibition curve (24.2+/-3.5% of the ET-1 response) was defined at low (up to 1 microM) concentrations of BQ-123, yielding an estimated Ki value for BQ-123 of 21.3+/-2.5 nM. In addition, the presence of 1 microM BQ-123 significantly reduced the maximal response to ET-1 but did not change the pD2 value. Increasing concentrations of the ET(B) selective antagonist BQ-788 inhibited the S6c response with a Ki of 17.8+/-0.8 nM. BQ-788 also inhibited the effect of ET-1, although, in this case, two components were defined, accounting for approximately 50% of the response, and showing Ki values of 20.9+/-5.1 nM and 439+/-110 nM, respectively. The ET-1 concentration-response curve was shifted to the right by 1 microM BQ-788, also revealing two components. Only one of them, corresponding to 69.8+/-4.4% of the response, was sensitive to BQ-788 which showed a Ki value of 28.8+/-8.9 nM. Rapid desensitization was achieved by preincubation with ET-1 or S6c. In cells pretreated with S6c neither ET-3 nor S6c activated PLD, but ET-1 still induced approximately 40% of the response shown by non-desensitised cells. This remaining response was insensitive to BQ-788, but fully inhibited by BQ-123. In conclusion, endothelins activate PLD in rat cortical astrocytes acting through both ET(A) and ET(B) receptors, and this response desensitizes rapidly in an apparently homologous fashion. The percentage contribution of ET(A) and ET(B) receptors to the ET-1 response was found to be approximately 20% and 80%, respectively, when ET(B) receptors were not blocked, and 30-50% and 50-70%, respectively, when ET(B) receptors were inhibited or desensitized. These results may be relevant to the study of a possible role of PLD in the proliferative effects shown by endothelins on cultured and reactive astrocytes.

The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes.[Pubmed:2649896]

Proc Natl Acad Sci U S A. 1989 Apr;86(8):2863-7.

Three distinct human endothelin-related genes were cloned by screening a genomic DNA library under a low hybridization stringency with a synthetic oligonucleotide probe encoding a portion of the endothelin sequence. Genomic Southern blot analysis with the same oligonucleotide probe showed three corresponding chromosomal loci not only in the human genome but also in porcine and rat genomes. The nucleotide sequences of the three human genes were highly conserved within the regions encoding the 21-residue (mature) endothelins, in spite of the fact that the immediately upstream exon sequences, which encode a part of the propeptides, retained little similarity. Moreover, each of the human genes predicted a putative 21-residue peptide, similar to but distinct from each other: (i) the "classical" endothelin (ET-1), (ii) [Trp6,Leu7]endothelin (ET-2), and (iii) [Thr2,Phe4,Thr5,Tyr6, Lys7,Tyr14]endothelin (ET-3). Synthetic ET-1, ET-2, and ET-3 were prepared according to the deduced amino acid sequences, and the biological activities were assayed by contraction of isolated porcine coronary artery strips and by intravenous injection to anesthetized rats. All these synthetic peptides produced strong vasoconstrictor and pressor responses. However, the quantitative profiles of the pharmacological activities were considerably different among the three isopeptides, suggesting the possible existence of endothelin receptor subtypes.

Endothelin-3, human, mouse, rabbit, rat is a 21-amino acid vasoactive peptide that binds to G-protein-linked transmembrane receptors, ET-RA and ET-RB.

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