FormononetinCAS# 485-72-3 |
Quality Control & MSDS
Number of papers citing our products
Chemical structure
3D structure
Cas No. | 485-72-3 | SDF | Download SDF |
PubChem ID | 5280378 | Appearance | White powder |
Formula | C16H12O4 | M.Wt | 268.27 |
Type of Compound | Flavonoids | Storage | Desiccate at -20°C |
Synonyms | Biochanin B; Flavosil; Formononetol | ||
Solubility | DMSO : ≥ 35 mg/mL (130.47 mM) H2O : < 0.1 mg/mL (insoluble) *"≥" means soluble, but saturation unknown. | ||
Chemical Name | 7-hydroxy-3-(4-methoxyphenyl)chromen-4-one | ||
SMILES | COC1=CC=C(C=C1)C2=COC3=C(C2=O)C=CC(=C3)O | ||
Standard InChIKey | HKQYGTCOTHHOMP-UHFFFAOYSA-N | ||
Standard InChI | InChI=1S/C16H12O4/c1-19-12-5-2-10(3-6-12)14-9-20-15-8-11(17)4-7-13(15)16(14)18/h2-9,17H,1H3 | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | Formononetin is a phytoestrogen, could be used in postmenopausal osteoporosis. It has antitumorigenic effects, suppressed the proliferation of human non-small cell lung cancer through induction of cell cycle arrest and apoptosis; it also exhibits antiviral activities against some members of Picornaviridae, could inhibit EV71-induced COX-2 expression and PGE2 production via MAPKs pathway including ERK, p38 and JNK. |
Targets | COX | PGE | ERK | p38MAPK | JNK | Caspase | Bcl-2/Bax | p53 | p21 | Akt | CDK | NF-kB | NOS | Calcium Channel | ATPase | Potassium Channel |
In vitro | Formononetin inhibits enterovirus 71 replication by regulating COX- 2/PGE2 expression.[Pubmed: 25890183]Virol J. 2015 Mar 1;12(1):35.The activation of ERK, p38 and JNK signal cascade in host cells has been demonstrated to up-regulate of enterovirus 71 (EV71)-induced cyclooxygenase-2 (COX-2)/ prostaglandins E2 (PGE₂) expression which is essential for viral replication. So, we want to know whether a compound can inhibit EV71 infection by suppressing COX-2/PGE₂ expression.
Formononetin promotes proliferation that involves a feedback loop of microRNA-375 and estrogen receptor alpha in estrogen receptor-positive cells.[Pubmed: 25663261]Mol Carcinog. 2016 Mar;55(3):312-9.Formononetin is an O-methylated isoflavone that is isolated from the root of Astragalus membranaceus, and it has antitumorigenic effects. Our previous studies found that Formononetin triggered growth-inhibitory and apoptotic activities in MCF-7 breast cancer cells.
Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways.[Pubmed: 19570671 ]J Nutr Biochem. 2010 Jul;21(7):613-20.We evaluated the vasorelaxation effects of Formononetin, an isoflavone/phytoestrogen found abundantly in Astragalus mongholicus Bunge, on rat isolated aorta and the underlying mechanisms involved.
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In vivo | Formononetin promotes cell cycle arrest via downregulation of Akt/Cyclin D1/CDK4 in human prostate cancer cells.[Pubmed: 25301361]Cell Physiol Biochem. 2014;34(4):1351-8.Formononetin is an O-methylated isoflavone isolated from the root of Astragalus membranaceus. It has already been reported that Formononetin could inhibit cell proliferation and induce cell apoptosis in several cancers, including prostate cancer. This study aimed to further investigate whether cell cycle arrest is involved in Formononetin-mediated antitumor effect in human prostate cancer cells, along with the underlying molecular mechanism. |
Cell Research | Formononetin attenuates hydrogen peroxide (H2O2)-induced apoptosis and NF-κB activation in RGC-5 cells.[Pubmed: 25070826]Formononetin suppresses the proliferation of human non-small cell lung cancer through induction of cell cycle arrest and apoptosis.[Pubmed: 25674209]Int J Clin Exp Pathol. 2014 Dec 1;7(12):8453-61.Formononetin is a novel herbal isoflavonoid isolated from Astragalus membranaceus and possesses antitumorigenic properties.
Eur Rev Med Pharmacol Sci. 2014;18(15):2191-7.Diabetic retinopathy is a common diabetic eye disease caused by changes in retinal ganglion cells (RGCs). Several studies suggest that the oxidative stress plays a role in the pathogenesis of diabetic retinopathy in adults. Formononetin is a flavone with powerful antioxidant properties that exists naturally in various plants and Chinese medicine. In the present study, an attempt has been made to investigate the antioxidative effects of Formononetin on H2O2-induced apoptosis of RGC-5 cells.
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Animal Research | Formononetin reverses established osteopenia in adult ovariectomized rats.[Pubmed: 22781783]Menopause. 2012 Aug;19(8):856-63.Formononetin (Formo) prevents ovariectomy (Ovx)-induced bone loss in rats. However, there are no reports on the curative effects of Formo. The objective of this study was to investigate the ability of Formo in restoring trabecular microarchitecture and promoting new bone formation in osteopenic rats.
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Formononetin Dilution Calculator
Formononetin Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 3.7276 mL | 18.6379 mL | 37.2759 mL | 74.5518 mL | 93.1897 mL |
5 mM | 0.7455 mL | 3.7276 mL | 7.4552 mL | 14.9104 mL | 18.6379 mL |
10 mM | 0.3728 mL | 1.8638 mL | 3.7276 mL | 7.4552 mL | 9.319 mL |
50 mM | 0.0746 mL | 0.3728 mL | 0.7455 mL | 1.491 mL | 1.8638 mL |
100 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.7455 mL | 0.9319 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
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Formononetin (Formononetol; Flavosil) is a bioactive component extracted from the red clover; inhibits the proliferation of DU-145/PC-3 cells in a dose-dependent manner. IC50 value: Target: anti-cancer in vitro: formononetin inhibited the proliferation of DU-145 cells in a dose-dependent manner. DU-145 cells treated with different concentrations of formononetin displayed obvious morphological changes of apoptosis under fluorescence microscopy. In addition, formononetin increased the proportion of early apoptotic DU-145 cells, down-regulated the protein levels of Bcl-2 and up-regulated those of RASD1 and Bax [1]. Formononetin significantly inhibited the cell growth of PC-3 in a dose-dependent manner, but no such effect was observed in RWPE1 cells. Formononetin treatment contributed to the reduced Bcl-2 protein level and the elevated Bax expression in PC-3 cells, thereby resulting in the increasing Bax/Bcl-2 ratios. Furthermore, the phosphorylated level of p38 in PC-3 cells was activated through the FN treatment, whereas the endogenous Akt phosphorylation was blocked [2]. Compared with the control, formononetin inhibited the proliferation of MCF-7 cells and effectively induced cell cycle arrest. The levels of p-IGF-1?R, p-Akt, cyclin D1 protein expression, and cyclin D1 mRNA expression were also downregulated [3]. in vivo: formononetin also prevented the tumor growth of human breast cancer cells in nude mouse xenografts [3].
References:
[1]. Liu XJ, et al. Up-regulating of RASD1 and apoptosis of DU-145 human prostate cancer cells induced by formononetin in vitro. Asian Pac J Cancer Prev. 2014;15(6):2835-9.
[2]. Zhang X, et al. Formononetin induces apoptosis in PC-3 prostate cancer cells through enhancing the Bax/Bcl-2 ratios and regulating the p38/Akt pathway. Nutr Cancer. 2014;66(4):656-61.
[3]. Chen J, et al. Formononetin induces cell cycle arrest of human breast cancer cells via IGF1/PI3K/Akt pathways in vitro and in vivo. Horm Metab Res. 2011 Sep;43(10):681-6.
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Formononetin promotes cell cycle arrest via downregulation of Akt/Cyclin D1/CDK4 in human prostate cancer cells.[Pubmed:25301361]
Cell Physiol Biochem. 2014;34(4):1351-8.
BACKGROUND: Formononetin is an O-methylated isoflavone isolated from the root of Astragalus membranaceus. It has already been reported that Formononetin could inhibit cell proliferation and induce cell apoptosis in several cancers, including prostate cancer. This study aimed to further investigate whether cell cycle arrest is involved in Formononetin-mediated antitumor effect in human prostate cancer cells, along with the underlying molecular mechanism. METHODS: Human prostate cancer cells PC-3 and DU145 were respectively treated with various concentrations of Formononetin. The inhibitory effect of Formononetin on proliferation of prostate cancer cells was determined using MTT assays and flow cytometry. Next, Formononetin-induced alterations in cyclin D1, CDK4 and Akt expression in PC-3 cells were detected by real-time PCR and western blot. RESULTS: Formononetin dose-dependently inhibited prostate cancer cell proliferation via the induction of cell cycle arrest at G0/G1 phase in vitro, which was more evident in PC-3 cells. Meanwhile, concomitant with reduced phosphorylation of Akt in PC-3 cells, Formononetin remarkably downregulated expression levels of cyclin D1 and CDK4 in a dose-dependent manner. More interestingly, in the in vivo studies, Formononetin showed a noticeable inhibition of tumor growth in recipient mice. CONCLUSION: Formononetin could exhibit inhibitory activity against human prostate cancer cells in vivo and in vitro, which is associated with G1 cell cycle arrest by inactivation of Akt/cyclin D1/CDK4. Therefore, Formononetin may be used as a candidate agent for clinical treatment of prostate cancer in the future.
Formononetin attenuates hydrogen peroxide (H2O2)-induced apoptosis and NF-kappaB activation in RGC-5 cells.[Pubmed:25070826]
Eur Rev Med Pharmacol Sci. 2014;18(15):2191-7.
OBJECTIVES: Diabetic retinopathy is a common diabetic eye disease caused by changes in retinal ganglion cells (RGCs). Several studies suggest that the oxidative stress plays a role in the pathogenesis of diabetic retinopathy in adults. Formononetin is a flavone with powerful antioxidant properties that exists naturally in various plants and Chinese medicine. In the present study, an attempt has been made to investigate the antioxidative effects of Formononetin on H2O2-induced apoptosis of RGC-5 cells. MATERIALS AND METHODS: Exposure of retinal ganglion cells (RGCs) to the indicated concentrations of Formononetin and H2O2 for 24 h, analyzed by MTT assay. Cells were stained with Annexin V-FITC and PI, analyzed by flow cytometry. And the level of superoxide anions, malondialdehyde (MDA, a marker of lipid peroxidation), 8-hydroxy-2-deoxyguanosine (8-OHdG, indicator of oxidative DNA damage) and MnSOD (manganese superoxide dismutase) activity were measured by kits. RESULTS: Formononetin reduced hydrogen peroxide (H2O2)-induced apoptosis and improved the levels or activity of indicators of oxidative stress. Formononetin also inhibited the activation of nuclear factor-kappaB (NF-kappaB), which is a significant transcription factor for RGC-5 apoptosis. CONCLUSIONS: Formononetin may be developed as a antioxidant drug to treat diabetic retinopathy.
Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways.[Pubmed:19570671]
J Nutr Biochem. 2010 Jul;21(7):613-20.
We evaluated the vasorelaxation effects of Formononetin, an isoflavone/phytoestrogen found abundantly in Astragalus mongholicus Bunge, on rat isolated aorta and the underlying mechanisms involved. Cumulative administration of Formononetin, genistein, daidzein and biochanin A relaxed phenylephrine-preconstricted aorta. Formononetin and biochanin A caused a similar magnitude of relaxation whereas daidzein was least potent. Mechanical removal of endothelium, L-NAME (100 microM) and methylene blue (10 microM) suppressed Formononetin-induced relaxation. Formononetin increased endothelial nitric oxide (NO) synthase (eNOS), but not inducible NO synthase, activity with an up-regulation of eNOS mRNA and p-eNOS(Ser1177) protein expression. In endothelium-denuded preparations, Formononetin-induced vasorelaxation was significantly reduced by glibenclamide (3 microM) and iberiotoxin (100 nM), and a combination of glibenclamide (3 microM) plus iberiotoxin (100 nM) abolished the relaxation. In contrast, Formononetin-elicited endothelium-independent relaxation was not altered by ICI 182,780 (10 microM, an estrogen receptor (ER alpha/ER beta) antagonist) or mifepristone (10 microM, a progesterone receptor antagonist). In single aortic smooth muscle cells, Formononetin caused opening of iberiotoxin-sensitive Ca(2+)-activated K(+) (BK(Ca)) channels and glibenclamide-sensitive adenosine triphosphate (ATP)-dependent K(+) (K(ATP)) channels. Thus, our results suggest that Formononetin caused vascular relaxation via endothelium/NO-dependent mechanism and endothelium-independent mechanism which involves the activation of BK(Ca) and K(ATP) channels.
Formononetin inhibits enterovirus 71 replication by regulating COX- 2/PGE(2) expression.[Pubmed:25890183]
Virol J. 2015 Mar 1;12:35.
BACKGROUND: The activation of ERK, p38 and JNK signal cascade in host cells has been demonstrated to up-regulate of enterovirus 71 (EV71)-induced cyclooxygenase-2 (COX-2)/ prostaglandins E2 (PGE(2)) expression which is essential for viral replication. So, we want to know whether a compound can inhibit EV71 infection by suppressing COX-2/PGE(2) expression. METHODS: The antiviral effect of Formononetin was determined by cytopathic effect (CPE) assay and the time course assays. The influence of Formononetin for EV71 replication was determined by immunofluorescence assay, western blotting assay and qRT-PCR assay. The mechanism of the antiviral activity of Formononetin was determined by western blotting assay and ELISA assay. RESULTS: Formononetin could reduce EV71 RNA and protein synthesis in a dose-dependent manner. The time course assays showed that Formononetin displayed significant antiviral activity both before (24 or 12 h) and after (0-6 h) EV71 inoculation in SK-N-SH cells. Formononetin was also able to prevent EV71-induced cytopathic effect (CPE) and suppress the activation of ERK, p38 and JNK signal pathways. Furthermore, Formononetin could suppress the EV71-induced COX-2/PGE(2) expression. Also, Formononetin exhibited similar antiviral activities against other members of Picornaviridae including coxsackievirus B2 (CVB2), coxsackievirus B3 (CVB3) and coxsackievirus B6 (CVB6). CONCLUSIONS: Formononetin could inhibit EV71-induced COX-2 expression and PGE(2) production via MAPKs pathway including ERK, p38 and JNK. Formononetin exhibited antiviral activities against some members of Picornaviridae. These findings suggest that Formononetin could be a potential lead or supplement for the development of new anti-EV71 agents in the future.
Formononetin suppresses the proliferation of human non-small cell lung cancer through induction of cell cycle arrest and apoptosis.[Pubmed:25674209]
Int J Clin Exp Pathol. 2014 Dec 1;7(12):8453-61. eCollection 2014.
Formononetin is a novel herbal isoflavonoid isolated from Astragalus membranaceus and possesses antitumorigenic properties. In the present study, we investigated the anti-proliferative effects of Formononetin on human non-small cell lung cancer (NSCLC), and further elucidated the molecular mechanism underlying the anti-tumor property. MTT assay showed that Formononetin treatment significantly inhibited the proliferation of two NSCLC cell lines including A549 and NCI-H23 in a time- and dose-dependent manner. Flow cytometric analysis demonstrated that Formononetin induced G1-phase cell cycle arrest and promoted cell apoptosis in NSCLC cells. On the molecular level, we observed that exposure to Formononetin altered the expression levels of cell cycle arrest-associated proteins p21, cyclin A and cyclin D1. Meanwhile, the apoptosis-related proteins cleaved caspase-3, bax and bcl-2 were also changed following treatment with Formononetin. In addition, the expression level of p53 was dose-dependently upregulated after administration with Formononetin. We also found that Formononetin treatment increased the phosphorylation of p53 at Ser15 and Ser20 and enhances its transcriptional activity in a dose-dependent manner. Collectively, these results demonstrated that Formononetin might be a potential chemopreventive drug for lung cancer therapy through induction of cell cycle arrest and apoptosis in NSCLC cells.
Formononetin promotes proliferation that involves a feedback loop of microRNA-375 and estrogen receptor alpha in estrogen receptor-positive cells.[Pubmed:25663261]
Mol Carcinog. 2016 Mar;55(3):312-9.
Formononetin is an O-methylated isoflavone that is isolated from the root of Astragalus membranaceus, and it has antitumorigenic effects. Our previous studies found that Formononetin triggered growth-inhibitory and apoptotic activities in MCF-7 breast cancer cells. To further investigate the potential effect of Formononetin in promoting cell proliferation in estrogen receptor (ER)-positive cells, we used in vivo and in vitro studies to elucidate the possible mechanism. ERalpha-positive cells (HUVEC, MCF-7) were treated with Formononetin. The CCK8 assay, Hoechst 33258, and flow cytometry were used to assess cell proliferation and apoptosis. mRNA levels of ERalpha, Bcl-2, and miR-375 were quantified using real-time polymerase chain reaction. ERalpha, p-Akt, and Bcl-2 expression was determined using Western blot. Compared with the control, low Formononetin concentrations (2-6 muM) stimulated ERalpha-positive cell proliferation (HUVEC, MCF-7). The more sensitive HUVEC cells were used to study the relevant signaling pathway. After treatment with Formononetin, ERalpha, miR-375, p-Akt, and Bcl-2 expression was significantly upregulated. The proliferative effect of Formononetin was also blocked by a miR-375 inhibitor or raloxifene pretreatment. Additionally, in the in vivo studies, uterine weight in ovariectomized mice treated with Formononetin increased significantly, but the weight dramatically decreased with raloxifene or miR-375 inhibitor pretreatment before Formononetin. This study demonstrated that Formononetin promoted ERalpha-positive cell proliferation through miR-375 activation and this mechanism is possibly involving in a miR-375 and ERalpha feedback loop.
Formononetin reverses established osteopenia in adult ovariectomized rats.[Pubmed:22781783]
Menopause. 2012 Aug;19(8):856-63.
OBJECTIVE: Formononetin (Formo) prevents ovariectomy (Ovx)-induced bone loss in rats. However, there are no reports on the curative effects of Formo. The objective of this study was to investigate the ability of Formo in restoring trabecular microarchitecture and promoting new bone formation in osteopenic rats. METHODS: Adult Sprague-Dawley rats were ovariectomized and left for 90 days for osteopenia to develop. After 90 days, Formo (10.0 mg kg d) was given orally for the next 12 weeks to Ovx rats in a therapeutic protocol. Sham-operated, Ovx + vehicle, and Ovx + parathyroid hormone (PTH) groups served as controls. Trabecular microarchitecture, osteoid formation, bone turnover/resorption markers, and bone osteoprotegerin-to-receptor activator for nuclear kappaB ligand ratio were studied. One-way analysis of variance was used to test significance of effects. RESULTS: Formo treatment significantly restored the lost trabecular microarchitecture in the femurs and tibia of osteopenic Ovx rats and promoted new bone formation. Formo was devoid of any uterine estrogenicity. Serum levels of type I collagen N-terminal propeptide, which is a reliable marker of bone formation, were increased in Ovx rats treated with Formo compared with Ovx + vehicle group, and the levels were comparable with those in the sham group. Formo prevented the Ovx-induced increase in bone turnover markers, including serum osteocalcin and urinary type I collagen degradation product. Furthermore, Formo-treated Ovx rats had an increased bone osteoprotegerin-to-receptor activator for nuclear kappaB ligand ratio compared with the Ovx + vehicle group. CONCLUSIONS: Daily oral administration of Formo for 12 weeks has a substantial anabolic effect, thus raising the possibility of its use in postmenopausal osteoporosis.