Gypenoside A

CAS# 157752-01-7

Gypenoside A

2D Structure

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Quality Control of Gypenoside A

3D structure

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Gypenoside A

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Chemical Properties of Gypenoside A

Cas No. 157752-01-7 SDF Download SDF
PubChem ID 131849056 Appearance White crystalline powder
Formula C46H74O17 M.Wt 899.07
Type of Compound Triterpenoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 17-[2,3-dihydroxy-5-(2-methylprop-1-enyl)oxolan-3-yl]-3-[5-hydroxy-3-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxy-4-(3,4,5-trihydroxyoxan-2-yl)oxyoxan-2-yl]oxy-4,4,8,14-tetramethyl-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-10-carbaldehyde
SMILES CC1C(C(C(C(O1)OC2C(C(COC2OC3CCC4(C5CCC6C(CCC6(C5(CCC4C3(C)C)C)C)C7(CC(OC7O)C=C(C)C)O)C=O)O)OC8C(C(C(CO8)O)O)O)O)O)O
Standard InChIKey JJURGRVZASRDDE-UHFFFAOYSA-N
Standard InChI InChI=1S/C46H74O17/c1-21(2)16-23-17-46(56,41(55)60-23)25-10-13-43(6)24(25)8-9-29-44(43,7)14-11-28-42(4,5)30(12-15-45(28,29)20-47)61-40-37(63-39-35(54)33(52)31(50)22(3)59-39)36(27(49)19-58-40)62-38-34(53)32(51)26(48)18-57-38/h16,20,22-41,48-56H,8-15,17-19H2,1-7H3
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Gypenoside A

The herbs of Gynostemma pentaphyllum (Thunb.) Makino.

Protocol of Gypenoside A

Structure Identification
Zhong Yao Cai. 1997 Aug;20(8):398-400.

Determination on the glycosyl sequence of gypenoside A by TLC-FABMS.[Pubmed: 12572416]


METHODS AND RESULTS:
The glycosyl sequence of Gypenoside A (A) isolated from Gynostemma pentaphyllum Makino was determinated by TLC-FABMS. The Gypenoside A was hydrolysed with cellulase and the hydrolysate showed eight spots on a TLC plate, and the FABMS analysis of these spots indicated they corresponded to Gypenoside A, A-Rham, A-Glu, A-Rham-Glu, A-Rham-Glu, A-Glu-Glu, A-Rham-2Glu, A-Rham-2Glu in order. Two chains can be determined to be Rham-Glu, and Glu-Glu, respectively.
CONCLUSIONS:
So the glycosyl sequence of Gypenoside A was identical of gypenoside XLIII.

Gypenoside A Dilution Calculator

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Gypenoside A Molarity Calculator

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Preparing Stock Solutions of Gypenoside A

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.1123 mL 5.5613 mL 11.1226 mL 22.2452 mL 27.8065 mL
5 mM 0.2225 mL 1.1123 mL 2.2245 mL 4.449 mL 5.5613 mL
10 mM 0.1112 mL 0.5561 mL 1.1123 mL 2.2245 mL 2.7807 mL
50 mM 0.0222 mL 0.1112 mL 0.2225 mL 0.4449 mL 0.5561 mL
100 mM 0.0111 mL 0.0556 mL 0.1112 mL 0.2225 mL 0.2781 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Description

Gypenoside A is a natural compound isolaated from Gynostemma pentaphyllum Makino.

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