H-Asp(OEt)-OEt.HCl

CAS# 16115-68-7

H-Asp(OEt)-OEt.HCl

2D Structure

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Chemical Properties of H-Asp(OEt)-OEt.HCl

Cas No. 16115-68-7 SDF Download SDF
PubChem ID 12416466 Appearance Powder
Formula C8H15NO4 M.Wt 189.2
Type of Compound N/A Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name diethyl (2S)-2-aminobutanedioate;hydrochloride
SMILES CCOC(=O)CC(C(=O)OCC)N.Cl
Standard InChIKey AJOXZAAREAYBQR-RGMNGODLSA-N
Standard InChI InChI=1S/C8H15NO4.ClH/c1-3-12-7(10)5-6(9)8(11)13-4-2;/h6H,3-5,9H2,1-2H3;1H/t6-;/m0./s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

H-Asp(OEt)-OEt.HCl Dilution Calculator

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Preparing Stock Solutions of H-Asp(OEt)-OEt.HCl

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 5.2854 mL 26.4271 mL 52.8541 mL 105.7082 mL 132.1353 mL
5 mM 1.0571 mL 5.2854 mL 10.5708 mL 21.1416 mL 26.4271 mL
10 mM 0.5285 mL 2.6427 mL 5.2854 mL 10.5708 mL 13.2135 mL
50 mM 0.1057 mL 0.5285 mL 1.0571 mL 2.1142 mL 2.6427 mL
100 mM 0.0529 mL 0.2643 mL 0.5285 mL 1.0571 mL 1.3214 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on H-Asp(OEt)-OEt.HCl

Total enzymatic synthesis of cholecystokinin CCK-5.[Pubmed:15309578]

Amino Acids. 2004 Aug;27(1):101-5.

This paper describes the enzymatic synthesis of the C-terminal fragment H-Gly-Trp-Met-Asp-Phe-NH2 of cholecystokinin. Immobilized enzymes were used for the formation of all peptide bonds except thermolysin. Beginning the synthesis with phenylacetyl (PhAc) glycine carboxamidomethyl ester (OCam) and H-Trp-OMe by using immobilized papain as biocatalyst in buffered ethyl acetate, the dipeptide methyl ester was then coupled directly with Met-OEt.HCl by alpha-chymotrypsin/Celite 545 in a solvent free system. For the 3+2 coupling PhAc-Gly-Trp-Met-OEt had to be converted into its OCam ester. The other fragment H-Asp(OMe)-Phe-NH2 resulted from the coupling of Cbo-Asp(OMe)-OH with H-Phe-NH2.HCl and thermolysin as catalyst, followed by catalytic hydrogenation. Finally PhAc-Gly-Trp-Met-Asp-Phe-NH2 was obtained in a smooth reaction from PhAc-Gly-Trp-Met-OCam and H-Asp(OMe)-Phe-NH2 with alpha-chymotrypsin/Celite 545 in acetonitrile, followed by basic hydrolysis of the beta-methyl ester. The PhAc-group is removed with penicillin G amidase and CCK-5 is obtained in an overall isolated yield of 19.6%.

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