ISX 9

Neurogenic agent; induces neuronal differentiation of SVZ progenitors and also induces cardiomyogenic differentiation CAS# 832115-62-5

ISX 9

Catalog No. BCC6181----Order now to get a substantial discount!

Product Name & Size Price Stock
ISX 9: 5mg $92 In Stock
ISX 9: 10mg Please Inquire In Stock
ISX 9: 20mg Please Inquire Please Inquire
ISX 9: 50mg Please Inquire Please Inquire
ISX 9: 100mg Please Inquire Please Inquire
ISX 9: 200mg Please Inquire Please Inquire
ISX 9: 500mg Please Inquire Please Inquire
ISX 9: 1000mg Please Inquire Please Inquire
Related Products
  • CGS 21680

    Catalog No.:BCC1475
    CAS No.:120225-54-9
  • Preladenant

    Catalog No.:BCC1868
    CAS No.:377727-87-2
  • Pentostatin

    Catalog No.:BCC1845
    CAS No.:53910-25-1
  • Tozadenant

    Catalog No.:BCC2011
    CAS No.:870070-55-6
  • LUF6000

    Catalog No.:BCC1710
    CAS No.:890087-21-5

Quality Control of ISX 9

Number of papers citing our products

Chemical structure

ISX 9

3D structure

Chemical Properties of ISX 9

Cas No. 832115-62-5 SDF Download SDF
PubChem ID 19582717 Appearance Powder
Formula C11H10N2O2S M.Wt 234.27
Type of Compound N/A Storage Desiccate at -20°C
Synonyms Isoxazole 9
Solubility DMSO : ≥ 37 mg/mL (157.94 mM)
H2O : < 0.1 mg/mL (insoluble)
*"≥" means soluble, but saturation unknown.
Chemical Name N-cyclopropyl-5-thiophen-2-yl-1,2-oxazole-3-carboxamide
SMILES C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3
Standard InChIKey SYENTKHGMVKGAQ-UHFFFAOYSA-N
Standard InChI InChI=1S/C11H10N2O2S/c14-11(12-7-3-4-7)8-6-9(15-13-8)10-2-1-5-16-10/h1-2,5-7H,3-4H2,(H,12,14)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of ISX 9

DescriptionNeurogenic agent. Mediates neuroD reporter gene induction via activation of Ca2+ influx; increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. Induces neuronal differentiation in human cortical neuronal cells (HCN), adult mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. Also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro.

ISX 9 Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

ISX 9 Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of ISX 9

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 4.2686 mL 21.3429 mL 42.6858 mL 85.3716 mL 106.7145 mL
5 mM 0.8537 mL 4.2686 mL 8.5372 mL 17.0743 mL 21.3429 mL
10 mM 0.4269 mL 2.1343 mL 4.2686 mL 8.5372 mL 10.6714 mL
50 mM 0.0854 mL 0.4269 mL 0.8537 mL 1.7074 mL 2.1343 mL
100 mM 0.0427 mL 0.2134 mL 0.4269 mL 0.8537 mL 1.0671 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on ISX 9

ISX-9 is a small molecule inducer of adult neural stem cell differentiation. Target: At 2.5-20 μM, ISX-9 has been shown to dose-dependently trigger neurogenesis and block gliogenesis in adult rat hippocampal stem cells through a calcium-activated signaling pathway dependent on myocyte-enhancer factor 2-dependent gene expression.ISX-9 administered at 20 mg/kg for 12 days to mice has been reported to improve hippocampal function as evidenced by enhanced spatial memory ability in the Morris water maze test.

References:
[1]. Petrik D, et al. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB J. 2012 Aug;26(8):3148-3162.

Featured Products
New Products
 

References on ISX 9

ISX-9 can potentiate cell proliferation and neuronal commitment in the rat dentate gyrus.[Pubmed:27373772]

Neuroscience. 2016 Sep 22;332:212-22.

Adult hippocampal neurogenesis can be modulated by various physiological and pathological conditions, including stress, affective disorders, and several neurological conditions. Given the proposed role of this form of structural plasticity in the functioning of the hippocampus (namely learning and memory and affective behaviors), it is believed that alterations in hippocampal neurogenesis might underlie some of the behavioral deficits associated with these psychiatric and neurological conditions. Thus, the search for compounds that can reverse these deficits with minimal side effects has become a recognized priority. In the present study we tested the pro-neurogenic effects of isoxazole 9 (Isx-9), a small synthetic molecule that has been recently identified through the screening of chemical libraries in stem cell-based assays. We found that administration of Isx-9 for 14days was able to potentiate cell proliferation and increase the number of immature neurons in the hippocampal DG of adult rats. In addition, Isx-9 treatment was able to completely reverse the marked reduction in these initial stages of the neurogenic process observed in vehicle-treated animals (which were submitted to repeated handling and exposure to daily intraperitoneal injections). Based on these results, we recommend that future neurogenesis studies that require repeated handling and manipulation of animals should include a naive (non-manipulated) control to determine the baseline levels of hippocampal cell proliferation and neuronal differentiation. Overall, these findings demonstrate that Isx-9 is a promising synthetic compound for the mitigation of stress-induced deficits in adult hippocampal neurogenesis. Future studies are thus warranted to evaluate the pro-neurogenic properties of Isx-9 in animal models of affective and neurological disorders associated with impaired hippocampal structural plasticity.

Targeting native adult heart progenitors with cardiogenic small molecules.[Pubmed:22413910]

ACS Chem Biol. 2012 Jun 15;7(6):1067-76.

Targeting native progenitors with small molecule pharmaceuticals that direct cell fate decisions is an attractive approach for regenerative medicine. Here, we show that 3,5-disubstituted isoxazoles (Isx), stem cell-modulator small molecules originally recovered in a P19 embryonal carcinoma cell-based screen, directed cardiac muscle gene expression in vivo in target tissues of adult transgenic reporter mice. Isx also stimulated adult mouse myocardial cell cycle activity. Narrowing our focus onto one target cardiac-resident progenitor population, Isx directed muscle transcriptional programs in vivo in multipotent Notch-activated epicardium-derived cells (NECs), generating Notch-activated adult cardiomyocyte-like precursors. Myocardial infarction (MI) preemptively differentiated NECs toward fibroblast lineages, overriding Isx's cardiogenic influence in this cell population. Isx dysregulated gene expression in vivo in Notch-activated repair fibroblasts, driving distinctive (pro-angiogenesis) gene programs, but failed to mitigate fibrosis or avert ventricular functional decline after MI. In NECs in vitro, Isx directed partial muscle differentiation, which included biosynthesis and assembly of sarcomeric alpha-actinin premyofibrils, beaded structures pathognomonic of early developing cardiomyocytes. Thus, although Isx small molecules have promising in vivo efficacy at the level of cardiac muscle gene expression in native multipotent progenitors and are first in class in this regard, a greater understanding of the dynamic interplay between fibrosis and cardiogenic small molecule signals will be required to pharmacologically enable regenerative repair of the heart.

A small molecule differentiation inducer increases insulin production by pancreatic beta cells.[Pubmed:22143803]

Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20713-8.

New drugs for preserving and restoring pancreatic beta-cell function are critically needed for the worldwide epidemic of type 2 diabetes and the cure for type 1 diabetes. We previously identified a family of neurogenic 3,5-disubstituted isoxazoles (Isx) that increased expression of neurogenic differentiation 1 (NeuroD1, also known as BETA2); this transcription factor functions in neuronal and pancreatic beta-cell differentiation and is essential for insulin gene transcription. Here, we probed effects of Isx on human cadaveric islets and MIN6 pancreatic beta cells. Isx increased the expression and secretion of insulin in islets that made little insulin after prolonged ex vivo culture and increased expression of neurogenic differentiation 1 and other regulators of islet differentiation and insulin gene transcription. Within the first few hours of exposure, Isx caused biphasic activation of ERK1/2 and increased bulk histone acetylation. Although there was little effect on histone deacetylase activity, Isx increased histone acetyl transferase activity in nuclear extracts. Reconstitution assays indicated that Isx increased the activity of the histone acetyl transferase p300 through an ERK1/2-dependent mechanism. In summary, we have identified a small molecule with antidiabetic activity, providing a tool for exploring islet function and a possible lead for therapeutic intervention in diabetes.

Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression.[Pubmed:21419563]

Differentiation. 2011 Apr;81(4):233-42.

In the central nervous system (CNS), neural stem cells (NSCs) differentiate into neurons, astrocytes, and oligodendrocytes--these cell lineages are considered unidirectional and irreversible under normal conditions. The introduction of a defined set of transcription factors has been shown to directly convert terminally differentiated cells into pluripotent stem cells, reinforcing the notion that preserving cellular identity is an active process. Indeed, recent studies highlight that tumor suppressor genes (TSGs) such as Ink4a/Arf and p53, control the barrier to efficient reprogramming, leaving open the question whether the same TSGs function to maintain the differentiated state. During malignancy or following brain injury, mature astrocytes have been reported to re-express neuronal genes and re-gain neurogenic potential to a certain degree, yet few studies have addressed the underlying mechanisms due to a limited number of cellular models or tools to probe this process. Here, we use a synthetic small-molecule (isoxazole) to demonstrate that highly malignant EGFRvIII-expressing Ink4a/Arf(-/-); Pten(-/-) astrocytes downregulated their astrocyte character, re-entered the cell cycle, and upregulated neuronal gene expression. As a collateral discovery, isoxazole small-molecules blocked tumor cell proliferation in vitro, a phenotype likely coupled to activation of neuronal gene expression. Similarly, histone deacetylase inhibitors induced neuronal gene expression and morphologic changes associated with the neuronal phenotype, suggesting the involvement of epigenetic-mediated gene activation. Our study assesses the contribution of specific genetic pathways underlying the de-differentiation potential of astrocytes and uncovers a novel pharmacological tool to explore astrocyte plasticity, which may bring insight to reprogramming and anti-tumor strategies.

Small-molecule activation of neuronal cell fate.[Pubmed:18552832]

Nat Chem Biol. 2008 Jul;4(7):408-10.

We probed an epigenetic regulatory path from small molecule to neuronal gene activation. Isoxazole small molecules triggered robust neuronal differentiation in adult neural stem cells, rapidly signaling to the neuronal genome via Ca(2+) influx. Ca(2+)-activated CaMK phosphorylated and mediated nuclear export of the MEF2 regulator HDAC5, thereby de-repressing neuronal genes. These results provide new tools to explore the epigenetic signaling circuitry specifying neuronal cell fate and new leads for neuro-regenerative drugs.

Description

ISX-9 is a small molecule inducer of adult neural stem cell differentiation.

Keywords:

ISX 9,832115-62-5,Isoxazole 9,Natural Products,Wnt/β-catenin, buy ISX 9 , ISX 9 supplier , purchase ISX 9 , ISX 9 cost , ISX 9 manufacturer , order ISX 9 , high purity ISX 9

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: