Pulchinenoside B

CAS# 135247-95-9

Pulchinenoside B

Catalog No. BCN6554----Order now to get a substantial discount!

Product Name & Size Price Stock
Pulchinenoside B: 5mg Please Inquire In Stock
Pulchinenoside B: 10mg Please Inquire In Stock
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Quality Control of Pulchinenoside B

Number of papers citing our products

Chemical structure

Pulchinenoside B

3D structure

Chemical Properties of Pulchinenoside B

Cas No. 135247-95-9 SDF Download SDF
PubChem ID 44567219 Appearance Powder
Formula C53H86O22 M.Wt 1075.25
Type of Compound Triterpenoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name [(2S,3R,4S,5S,6R)-6-[[(2R,3R,4R,5S,6R)-3,4-dihydroxy-6-(hydroxymethyl)-5-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl] (1R,3aS,5aR,5bR,7aR,8R,9S,11aR,11bR,13aR,13bR)-8-(hydroxymethyl)-5a,5b,8,11a-tetramethyl-1-prop-1-en-2-yl-9-[(2S,3R,4S,5S)-3,4,5-trihydroxyoxan-2-yl]oxy-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-3a-carboxylate
SMILES CC1C(C(C(C(O1)OC2C(OC(C(C2O)O)OCC3C(C(C(C(O3)OC(=O)C45CCC(C4C6CCC7C8(CCC(C(C8CCC7(C6(CC5)C)C)(C)CO)OC9C(C(C(CO9)O)O)O)C)C(=C)C)O)O)O)CO)O)O)O
Standard InChIKey DMCLHHBLEQZLOC-ARLBSWEISA-N
Standard InChI InChI=1S/C53H86O22/c1-22(2)24-10-15-53(48(67)75-47-41(65)37(61)35(59)28(72-47)20-69-44-42(66)38(62)43(27(18-54)71-44)74-46-40(64)36(60)33(57)23(3)70-46)17-16-51(6)25(32(24)53)8-9-30-49(4)13-12-31(73-45-39(63)34(58)26(56)19-68-45)50(5,21-55)29(49)11-14-52(30,51)7/h23-47,54-66H,1,8-21H2,2-7H3/t23-,24-,25+,26-,27+,28+,29+,30+,31-,32+,33-,34-,35+,36+,37-,38+,39+,40+,41+,42+,43+,44+,45-,46-,47-,49-,50-,51+,52+,53-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Pulchinenoside B

The herb of Pulsatilla chinensis (Bge.) Regel

Biological Activity of Pulchinenoside B

DescriptionPulchinenoside B is a natural product from Pulsatilla chinensis (Bge.) Regel.

Protocol of Pulchinenoside B

Structure Identification
Zhong Yao Cai. 2010 Apr;33(4):617-20.

Optimization of extraction technics of total saponins from Pulsatilla cernua.[Pubmed: 20845794]

The extraction condition of total saponins from Pulsatilla cenua by ultrasonic wave was optimized by single factor and orthogonal experiments.
METHODS AND RESULTS:
The largest absorbency of saponin was intended to be 470 nm by wavelength scan method with the Pulchinenoside B4 as control sample, the linear relationship was observed between the absorbency and the content of saponin in the range of 0 - 0.040 mg/mL. The optimal conditions of extraction was as following: 80% of alcohol concentration, 40 min of ultrasonic time, 1: 20 of solid to liquid ratio, 80 W of ultrasonic power and one time for extraction. Among them, alcohol had the most significant effect on the extraction of total saponins.
CONCLUSIONS:
The content of total saponins in Pulsatilla cernua was 4. 32% under the optimal condition. The method developed here is efficient, stable, accurate and repeatable.

Pulchinenoside B Dilution Calculator

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Pulchinenoside B Molarity Calculator

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Preparing Stock Solutions of Pulchinenoside B

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 0.93 mL 4.6501 mL 9.3002 mL 18.6003 mL 23.2504 mL
5 mM 0.186 mL 0.93 mL 1.86 mL 3.7201 mL 4.6501 mL
10 mM 0.093 mL 0.465 mL 0.93 mL 1.86 mL 2.325 mL
50 mM 0.0186 mL 0.093 mL 0.186 mL 0.372 mL 0.465 mL
100 mM 0.0093 mL 0.0465 mL 0.093 mL 0.186 mL 0.2325 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Pulchinenoside B

[Optimization of extraction technics of total saponins from Pulsatilla cernua].[Pubmed:20845794]

Zhong Yao Cai. 2010 Apr;33(4):617-20.

OBJECTIVE: The extraction condition of total saponins from Pulsatilla cenua by ultrasonic wave was optimized by single factor and orthogonal experiments. METHODS: The largest absorbency of saponin was intended to be 470 nm by wavelength scan method with the Pulchinenoside B4 as control sample, the linear relationship was observed between the absorbency and the content of saponin in the range of 0 - 0.040 mg/mL. RESULTS: The optimal conditions of extraction was as following: 80% of alcohol concentration, 40 min of ultrasonic time, 1: 20 of solid to liquid ratio, 80 W of ultrasonic power and one time for extraction. Among them, alcohol had the most significant effect on the extraction of total saponins. CONCLUSION: The content of total saponins in Pulsatilla cernua was 4. 32% under the optimal condition. The method developed here is efficient, stable, accurate and repeatable.

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