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Aflatoxin B1

CAS# 1162-65-8

Aflatoxin B1

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Quality Control of Aflatoxin B1

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Chemical structure

Aflatoxin B1

3D structure

Chemical Properties of Aflatoxin B1

Cas No. 1162-65-8 SDF Download SDF
PubChem ID 186907 Appearance Powder
Formula C17H12O6 M.Wt 312.3
Type of Compound N/A Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
SMILES COC1=C2C3=C(C(=O)CC3)C(=O)OC2=C4C5C=COC5OC4=C1
Standard InChIKey OQIQSTLJSLGHID-WNWIJWBNSA-N
Standard InChI InChI=1S/C17H12O6/c1-20-10-6-11-14(8-4-5-21-17(8)22-11)15-13(10)7-2-3-9(18)12(7)16(19)23-15/h4-6,8,17H,2-3H2,1H3/t8-,17+/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Aflatoxin B1 Dilution Calculator

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Aflatoxin B1 Molarity Calculator

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Preparing Stock Solutions of Aflatoxin B1

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.202 mL 16.0102 mL 32.0205 mL 64.041 mL 80.0512 mL
5 mM 0.6404 mL 3.202 mL 6.4041 mL 12.8082 mL 16.0102 mL
10 mM 0.3202 mL 1.601 mL 3.202 mL 6.4041 mL 8.0051 mL
50 mM 0.064 mL 0.3202 mL 0.6404 mL 1.2808 mL 1.601 mL
100 mM 0.032 mL 0.1601 mL 0.3202 mL 0.6404 mL 0.8005 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Aflatoxin B1

Natural occurrence of mycotoxins in maize and sorghum in Togo.[Pubmed:30968272]

Mycotoxin Res. 2019 Apr 9. pii: 10.1007/s12550-019-00351-1.

Mycotoxins are fungal secondary metabolites frequently affecting agronomical crops and consequently imposing a major challenge for food safety and public health. In this study, a total of 67 raw cereals (55 maize and 12 sorghum) were collected from the market of Togo. The samples were investigated on the occurrence of 21 mycotoxins using state-of-the-art high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The most frequent occurring mycotoxins were fumonisins (88 and 67% for maize and sorghum respectively) with concentrations ranging from 101 to 1838 mug/kg for maize and 81.5 to 361 mug/kg for sorghum, respectively. Aflatoxin B1 was detected in 38% of the maize samples with maximum contamination levels of 256 mug/kg, and 25% of the sorghum samples (range 6-16 mug/kg). The concentrations of aflatoxins were high in maize, with some cases exceeding the maximum legislative limits (EU) for unprocessed maize placed on the market. In addition to these high contamination levels, the co-occurrence of three classes of mycotoxins (i.e., aflatoxins, fumonisins, and trichothecenes) was observed in this study. For the first time, the multi-mycotoxins occurrence in agronomical crops in Togo was reported.

Distribution and Effects of Aflatoxin in Chicken Tissues After Feeding Radiolabeled ((14)C) Aflatoxin B1.[Pubmed:30959599]

J Food Prot. 1986 Oct;49(10):799-805.

The effects of single oral administration of 1 mg of (14)C-Aflatoxin B1 to 3-wk-old chickens and 10 mg of non-radioactive aflatoxin to 6-wk-old chickens were determined. Analyses were made of the distribution of (14)C in the blood, organs, tissues and feces. No toxic effects were observed on chickens fed 1 mg of Aflatoxin B1 during 72 h but those fed 10 mg of non-radioactive aflatoxin exhibited both altered physical signs and pathological findings. Both the (14)C-aflatoxin studies and chemical analyses for the parent compound indicated that most of the aflatoxin was excreted in the feces within 48 h and of the amount retained, most was found in the liver.

Application of ELISA to Retail Survey of Aflatoxin B1 in Peanut Butter.[Pubmed:30959593]

J Food Prot. 1986 Oct;49(10):792-795.

A simple procedure was devised for the routine screening of Aflatoxin B1 (AFB1) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB1 and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB1 added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 mug/kg) of AFB1.

Simple "signal-on" photoelectrochemical aptasensor for ultrasensitive detecting AFB1 based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au nanocomposites.[Pubmed:30954925]

Biosens Bioelectron. 2019 Mar 28;134:42-48.

A simple "signal-on" photoelectrochemical (PEC) aptasensor is constructed for Aflatoxin B1 (AFB1) detection based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au (erGO/P5FIn/Au) nanocomposites. The nanocomposites are synthesized by simple electrochemical deposition method and show good photoelectrochemical performance. Poly(5-formylindole) (P5FIn) can generate electron-hole pairs under light irradiation, leading to the formation of robust cathode photocurrent. Au can be acted as signal amplifier due to the high conductivity. The erGO is used to immobilize AFB1 aptamer chain by pi-pi stacking interaction between the carbon six-membered ring in graphene and the C-N heterocyclic ring in nucleobases of ssDNA. After the insulating AFB1 aptamer chain is fixed to the electrode, the signal of PEC sensor is "OFF". In the process of AFB1 detection, the aptamer chain detaches from the surface of erGO, which results in "ON" of the sensor signal. Based on this design, this constructed PEC aptasensor shows a high sensitivity for AFB1 with a wide linear detection range (LDR) from 0.01ngmL(-1) to 100ngmL(-1). The limit of detection (LOD) is 0.002ngmL(-1). This PEC sensor also exhibits good stability, selectivity, specificity, and satisfactory practical sample analysis ability. This work may provide a new promising PEC platform for AFB1 detection as well as some other small molecules analysis.

Description

Aflatoxin B1 (AFB1) is a Class 1A carcinogen, which is a secondary metabolite of Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) mainly induces the transversion of G-->T in the third position of codon 249 of the p53 tumor suppressor gene, resulting in mutation.

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